Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdlA and mdlB overlap by 8 nucleotides mdlB and glnK are separated by 180 nucleotides glnK and amtB are separated by 29 nucleotides
b0448: mdlA - fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 468,095 to 469,867
mdlA
b0449: mdlB - fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 469,860 to 471,641
mdlB
b0450: glnK - nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI), at 471,822 to 472,160
glnK
b0451: amtB - ammonium transporter (NCBI), at 472,190 to 473,476
amtB
Position (kb) 469
470
471
472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 468.893 kb on + strand, within mdlA at 468.901 kb on + strand, within mdlA at 468.901 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.923 kb on - strand, within mdlA at 468.930 kb on + strand, within mdlA at 468.942 kb on + strand, within mdlA at 468.950 kb on - strand, within mdlA at 469.008 kb on + strand, within mdlA at 469.011 kb on - strand, within mdlA at 469.117 kb on + strand, within mdlA at 469.123 kb on - strand, within mdlA at 469.123 kb on - strand, within mdlA at 469.284 kb on + strand, within mdlA at 469.288 kb on - strand, within mdlA at 469.385 kb on + strand, within mdlA at 469.385 kb on + strand, within mdlA at 469.475 kb on + strand, within mdlA at 469.509 kb on - strand, within mdlA at 469.509 kb on - strand, within mdlA at 469.526 kb on + strand, within mdlA at 469.526 kb on + strand, within mdlA at 469.643 kb on - strand, within mdlA at 469.652 kb on - strand, within mdlA at 469.677 kb on + strand, within mdlA at 469.677 kb on + strand, within mdlA at 469.686 kb on + strand, within mdlA at 469.772 kb on - strand at 469.777 kb on - strand at 469.803 kb on - strand at 469.830 kb on - strand at 469.830 kb on - strand at 469.887 kb on + strand at 469.887 kb on + strand at 469.921 kb on + strand at 469.921 kb on + strand at 469.955 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 470.079 kb on - strand, within mdlB at 470.079 kb on - strand, within mdlB at 470.166 kb on + strand, within mdlB at 470.189 kb on - strand, within mdlB at 470.259 kb on - strand, within mdlB at 470.391 kb on + strand, within mdlB at 470.406 kb on - strand, within mdlB at 470.450 kb on + strand, within mdlB at 470.575 kb on - strand, within mdlB at 470.575 kb on - strand, within mdlB at 470.582 kb on + strand, within mdlB at 470.667 kb on + strand, within mdlB at 470.694 kb on + strand, within mdlB at 470.780 kb on + strand, within mdlB at 470.788 kb on - strand, within mdlB at 470.788 kb on - strand, within mdlB at 470.805 kb on - strand, within mdlB at 470.838 kb on + strand, within mdlB at 470.838 kb on + strand, within mdlB at 471.033 kb on - strand, within mdlB at 471.033 kb on - strand, within mdlB at 471.076 kb on - strand, within mdlB at 471.076 kb on - strand, within mdlB at 471.119 kb on - strand, within mdlB at 471.119 kb on - strand, within mdlB at 471.135 kb on + strand, within mdlB at 471.224 kb on + strand, within mdlB at 471.410 kb on - strand, within mdlB at 471.433 kb on + strand, within mdlB at 471.443 kb on + strand, within mdlB at 471.447 kb on - strand, within mdlB at 471.510 kb on - strand at 471.522 kb on - strand at 471.549 kb on + strand at 471.551 kb on + strand at 471.551 kb on + strand at 471.679 kb on + strand at 471.692 kb on - strand at 471.699 kb on - strand at 471.866 kb on + strand, within glnK at 471.866 kb on + strand, within glnK at 471.884 kb on - strand, within glnK at 472.069 kb on - strand, within glnK at 472.140 kb on + strand at 472.180 kb on - strand at 472.254 kb on - strand at 472.257 kb on + strand at 472.345 kb on + strand, within amtB at 472.345 kb on + strand, within amtB at 472.415 kb on - strand, within amtB at 472.417 kb on + strand, within amtB at 472.430 kb on - strand, within amtB at 472.444 kb on - strand, within amtB at 472.546 kb on + strand, within amtB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 468,893 + mdlA b0448 0.45 -1.9 468,901 + mdlA b0448 0.45 +0.2 468,901 - mdlA b0448 0.45 -0.9 468,914 - mdlA b0448 0.46 -0.5 468,914 - mdlA b0448 0.46 +0.2 468,914 - mdlA b0448 0.46 -0.8 468,914 - mdlA b0448 0.46 -0.8 468,923 - mdlA b0448 0.47 -1.4 468,930 + mdlA b0448 0.47 -0.7 468,942 + mdlA b0448 0.48 +3.5 468,950 - mdlA b0448 0.48 -0.8 469,008 + mdlA b0448 0.51 +0.0 469,011 - mdlA b0448 0.52 +0.1 469,117 + mdlA b0448 0.58 -1.9 469,123 - mdlA b0448 0.58 -0.4 469,123 - mdlA b0448 0.58 +1.4 469,284 + mdlA b0448 0.67 -0.9 469,288 - mdlA b0448 0.67 +0.8 469,385 + mdlA b0448 0.73 +0.5 469,385 + mdlA b0448 0.73 -1.4 469,475 + mdlA b0448 0.78 -0.5 469,509 - mdlA b0448 0.80 +2.2 469,509 - mdlA b0448 0.80 +0.2 469,526 + mdlA b0448 0.81 -0.8 469,526 + mdlA b0448 0.81 -1.9 469,643 - mdlA b0448 0.87 -1.8 469,652 - mdlA b0448 0.88 -0.4 469,677 + mdlA b0448 0.89 +0.2 469,677 + mdlA b0448 0.89 +0.9 469,686 + mdlA b0448 0.90 -0.9 469,772 - -1.4 469,777 - -1.8 469,803 - +0.6 469,830 - -0.4 469,830 - -1.1 469,887 + -0.4 469,887 + -0.2 469,921 + +2.5 469,921 + +0.7 469,955 - -1.4 469,965 - -0.4 469,965 - -2.2 469,965 - -1.6 469,965 - -1.4 470,079 - mdlB b0449 0.12 -1.8 470,079 - mdlB b0449 0.12 -0.1 470,166 + mdlB b0449 0.17 +1.2 470,189 - mdlB b0449 0.18 +0.2 470,259 - mdlB b0449 0.22 -1.6 470,391 + mdlB b0449 0.30 -2.6 470,406 - mdlB b0449 0.31 -2.6 470,450 + mdlB b0449 0.33 -1.5 470,575 - mdlB b0449 0.40 +1.1 470,575 - mdlB b0449 0.40 +1.4 470,582 + mdlB b0449 0.41 -2.9 470,667 + mdlB b0449 0.45 -2.1 470,694 + mdlB b0449 0.47 +0.2 470,780 + mdlB b0449 0.52 -0.6 470,788 - mdlB b0449 0.52 -1.4 470,788 - mdlB b0449 0.52 +0.2 470,805 - mdlB b0449 0.53 -1.4 470,838 + mdlB b0449 0.55 -2.1 470,838 + mdlB b0449 0.55 -1.8 471,033 - mdlB b0449 0.66 +0.8 471,033 - mdlB b0449 0.66 -0.4 471,076 - mdlB b0449 0.68 -1.9 471,076 - mdlB b0449 0.68 +0.2 471,119 - mdlB b0449 0.71 +0.2 471,119 - mdlB b0449 0.71 -0.6 471,135 + mdlB b0449 0.72 +0.1 471,224 + mdlB b0449 0.77 +1.2 471,410 - mdlB b0449 0.87 -0.4 471,433 + mdlB b0449 0.88 +0.2 471,443 + mdlB b0449 0.89 +0.2 471,447 - mdlB b0449 0.89 -1.1 471,510 - +0.9 471,522 - +0.2 471,549 + +0.6 471,551 + -0.4 471,551 + -0.2 471,679 + +2.3 471,692 - -0.1 471,699 - -1.1 471,866 + glnK b0450 0.13 +2.2 471,866 + glnK b0450 0.13 +1.8 471,884 - glnK b0450 0.18 -1.4 472,069 - glnK b0450 0.73 -0.5 472,140 + +0.1 472,180 - +0.2 472,254 - -0.8 472,257 + +1.6 472,345 + amtB b0451 0.12 +2.5 472,345 + amtB b0451 0.12 -1.9 472,415 - amtB b0451 0.17 +2.0 472,417 + amtB b0451 0.18 +0.4 472,430 - amtB b0451 0.19 -0.5 472,444 - amtB b0451 0.20 -0.4 472,546 + amtB b0451 0.28 +0.6
Or see this region's nucleotide sequence