Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sbcD and phoB are separated by 189 nucleotides phoB and phoR are separated by 57 nucleotides
b0398: sbcD - exonuclease, dsDNA, ATP-dependent (NCBI), at 414,974 to 416,176
sbcD
b0399: phoB - DNA-binding response regulator in two-component regulatory system with PhoR (or CreC) (NCBI), at 416,366 to 417,055
phoB
b0400: phoR - sensory histidine kinase in two-component regulatory system with PhoB (NCBI), at 417,113 to 418,408
phoR
Position (kb) 416
417
418 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 415.461 kb on + strand, within sbcD at 415.461 kb on + strand, within sbcD at 415.518 kb on + strand, within sbcD at 415.518 kb on + strand, within sbcD at 415.598 kb on + strand, within sbcD at 415.602 kb on + strand, within sbcD at 415.679 kb on + strand, within sbcD at 415.680 kb on + strand, within sbcD at 415.893 kb on - strand, within sbcD at 416.076 kb on + strand at 416.076 kb on + strand at 416.086 kb on - strand at 416.086 kb on - strand at 416.098 kb on - strand at 416.171 kb on + strand at 416.173 kb on + strand at 416.176 kb on - strand at 416.271 kb on - strand at 416.271 kb on - strand at 416.296 kb on - strand at 416.333 kb on - strand at 416.373 kb on + strand at 416.416 kb on + strand at 416.424 kb on - strand at 416.481 kb on - strand, within phoB at 416.481 kb on - strand, within phoB at 416.536 kb on - strand, within phoB at 416.562 kb on - strand, within phoB at 416.562 kb on - strand, within phoB at 416.654 kb on - strand, within phoB at 416.673 kb on - strand, within phoB at 416.676 kb on - strand, within phoB at 416.753 kb on - strand at 416.881 kb on + strand, within phoB at 416.937 kb on - strand, within phoB at 416.971 kb on + strand, within phoB at 416.990 kb on + strand at 416.993 kb on + strand at 417.003 kb on + strand at 417.008 kb on + strand at 417.015 kb on - strand at 417.015 kb on - strand at 417.015 kb on - strand at 417.016 kb on - strand at 417.022 kb on + strand at 417.076 kb on + strand at 417.076 kb on + strand at 417.091 kb on - strand at 417.144 kb on + strand at 417.174 kb on + strand at 417.270 kb on + strand, within phoR at 417.316 kb on - strand, within phoR at 417.389 kb on - strand, within phoR at 417.446 kb on + strand, within phoR at 417.446 kb on + strand, within phoR at 417.453 kb on - strand, within phoR at 417.538 kb on + strand, within phoR at 417.538 kb on + strand, within phoR at 417.541 kb on + strand, within phoR at 417.604 kb on + strand, within phoR at 417.616 kb on + strand, within phoR at 417.660 kb on - strand, within phoR at 417.660 kb on - strand, within phoR at 417.715 kb on - strand, within phoR at 417.715 kb on - strand, within phoR at 417.754 kb on - strand, within phoR at 417.754 kb on - strand, within phoR at 417.783 kb on + strand, within phoR at 417.889 kb on + strand, within phoR
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 415,461 + sbcD b0398 0.40 -3.0 415,461 + sbcD b0398 0.40 -2.2 415,518 + sbcD b0398 0.45 -1.1 415,518 + sbcD b0398 0.45 +1.4 415,598 + sbcD b0398 0.52 -2.1 415,602 + sbcD b0398 0.52 +0.7 415,679 + sbcD b0398 0.59 +1.0 415,680 + sbcD b0398 0.59 +1.6 415,893 - sbcD b0398 0.76 -2.4 416,076 + -0.9 416,076 + -2.3 416,086 - +2.7 416,086 - -0.5 416,098 - +0.8 416,171 + -2.4 416,173 + -1.7 416,176 - +3.0 416,271 - +1.6 416,271 - -1.7 416,296 - -0.5 416,333 - -0.7 416,373 + -0.3 416,416 + +0.8 416,424 - -1.3 416,481 - phoB b0399 0.17 -1.1 416,481 - phoB b0399 0.17 +0.0 416,536 - phoB b0399 0.25 -1.3 416,562 - phoB b0399 0.28 -0.9 416,562 - phoB b0399 0.28 -2.2 416,654 - phoB b0399 0.42 -1.3 416,673 - phoB b0399 0.44 -0.7 416,676 - phoB b0399 0.45 -0.7 416,753 - +0.3 416,881 + phoB b0399 0.75 +1.3 416,937 - phoB b0399 0.83 +1.7 416,971 + phoB b0399 0.88 -0.2 416,990 + +1.3 416,993 + -2.0 417,003 + -1.6 417,008 + -1.4 417,015 - +0.6 417,015 - +0.9 417,015 - -0.1 417,016 - +0.7 417,022 + +3.6 417,076 + +0.7 417,076 + -1.9 417,091 - +1.6 417,144 + -2.0 417,174 + -3.3 417,270 + phoR b0400 0.12 +0.3 417,316 - phoR b0400 0.16 -0.1 417,389 - phoR b0400 0.21 -0.2 417,446 + phoR b0400 0.26 +4.3 417,446 + phoR b0400 0.26 -1.6 417,453 - phoR b0400 0.26 -0.3 417,538 + phoR b0400 0.33 +1.3 417,538 + phoR b0400 0.33 -0.1 417,541 + phoR b0400 0.33 -1.6 417,604 + phoR b0400 0.38 -0.7 417,616 + phoR b0400 0.39 -2.8 417,660 - phoR b0400 0.42 -2.1 417,660 - phoR b0400 0.42 -0.3 417,715 - phoR b0400 0.46 -0.1 417,715 - phoR b0400 0.46 -0.3 417,754 - phoR b0400 0.49 -1.1 417,754 - phoR b0400 0.49 -0.9 417,783 + phoR b0400 0.52 +2.3 417,889 + phoR b0400 0.60 +1.2
Or see this region's nucleotide sequence