Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tauB and tauC overlap by 4 nucleotides tauC and tauD overlap by 4 nucleotides tauD and hemB are separated by 106 nucleotides
b0366: tauB - taurine transporter subunit (NCBI), at 385,431 to 386,198
tauB
b0367: tauC - taurine transporter subunit (NCBI), at 386,195 to 387,022
tauC
b0368: tauD - taurine dioxygenase (NCBI), at 387,019 to 387,870
tauD
b0369: hemB - 5-aminolevulinate dehydratase = porphobilinogen synthase (VIMSS), at 387,977 to 388,951
hemB
Position (kb) 387
388 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 386.055 kb on + strand, within tauB at 386.094 kb on + strand, within tauB at 386.245 kb on - strand at 386.275 kb on + strand at 386.297 kb on - strand, within tauC at 386.297 kb on - strand, within tauC at 386.532 kb on + strand, within tauC at 386.532 kb on + strand, within tauC at 386.562 kb on + strand, within tauC at 386.586 kb on - strand, within tauC at 386.729 kb on + strand, within tauC at 386.829 kb on - strand, within tauC at 386.958 kb on + strand at 387.043 kb on - strand at 387.266 kb on + strand, within tauD at 387.266 kb on + strand, within tauD at 387.410 kb on + strand, within tauD at 387.622 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.669 kb on - strand, within tauD at 387.678 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.783 kb on - strand, within tauD at 387.896 kb on + strand at 387.908 kb on + strand at 387.908 kb on + strand at 387.916 kb on - strand at 387.916 kb on - strand at 387.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 386,055 + tauB b0366 0.81 +1.9 386,094 + tauB b0366 0.86 +0.1 386,245 - -0.9 386,275 + -1.1 386,297 - tauC b0367 0.12 -0.3 386,297 - tauC b0367 0.12 -1.1 386,532 + tauC b0367 0.41 -2.1 386,532 + tauC b0367 0.41 -0.4 386,562 + tauC b0367 0.44 +1.8 386,586 - tauC b0367 0.47 -0.5 386,729 + tauC b0367 0.64 -1.1 386,829 - tauC b0367 0.77 -1.5 386,958 + +0.0 387,043 - -1.5 387,266 + tauD b0368 0.29 -0.1 387,266 + tauD b0368 0.29 +0.3 387,410 + tauD b0368 0.46 +1.8 387,622 + tauD b0368 0.71 -1.7 387,649 + tauD b0368 0.74 +1.3 387,649 + tauD b0368 0.74 +1.3 387,669 - tauD b0368 0.76 +2.5 387,678 + tauD b0368 0.77 +3.0 387,775 + tauD b0368 0.89 +1.5 387,775 + tauD b0368 0.89 -2.1 387,783 - tauD b0368 0.90 -1.7 387,896 + -0.4 387,908 + +0.9 387,908 + +0.4 387,916 - +0.7 387,916 - +0.3 387,916 - -1.2
Or see this region's nucleotide sequence