Strain Fitness in Escherichia coli BW25113 around b0355

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmhpT and yaiL are separated by 101 nucleotidesyaiL and frmB are separated by 223 nucleotidesfrmB and frmA are separated by 93 nucleotides b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894 mhpT b0354: yaiL - nucleoprotein/polynucleotide-associated enzyme (VIMSS), at 375,996 to 376,535 yaiL b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592 frmB b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795 frmA Position (kb) 376 377 378Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 375.780 kb on - strandat 375.780 kb on - strandat 375.806 kb on + strandat 375.898 kb on + strandat 375.920 kb on - strandat 375.921 kb on - strandat 375.921 kb on - strandat 375.940 kb on - strandat 375.940 kb on - strandat 376.196 kb on + strand, within yaiLat 376.196 kb on + strand, within yaiLat 376.199 kb on + strand, within yaiLat 376.199 kb on + strand, within yaiLat 376.312 kb on + strand, within yaiLat 376.331 kb on + strand, within yaiLat 376.331 kb on + strand, within yaiLat 376.331 kb on + strand, within yaiLat 376.336 kb on - strand, within yaiLat 376.336 kb on - strand, within yaiLat 376.366 kb on - strand, within yaiLat 376.366 kb on - strand, within yaiLat 376.376 kb on - strand, within yaiLat 376.376 kb on - strand, within yaiLat 376.384 kb on - strand, within yaiLat 376.406 kb on - strand, within yaiLat 376.425 kb on + strand, within yaiLat 376.449 kb on + strand, within yaiLat 376.449 kb on - strand, within yaiLat 376.457 kb on - strand, within yaiLat 376.481 kb on + strand, within yaiLat 376.489 kb on - strandat 376.489 kb on - strandat 376.489 kb on - strandat 376.539 kb on + strandat 376.539 kb on - strandat 376.558 kb on + strandat 376.618 kb on - strandat 376.653 kb on + strandat 376.682 kb on + strandat 376.684 kb on + strandat 376.684 kb on + strandat 376.692 kb on - strandat 376.719 kb on + strandat 376.745 kb on - strandat 376.745 kb on - strandat 376.747 kb on + strandat 376.755 kb on + strandat 376.771 kb on - strandat 376.783 kb on + strandat 376.853 kb on - strand, within frmBat 376.875 kb on + strand, within frmBat 376.906 kb on + strand, within frmBat 376.909 kb on + strand, within frmBat 376.909 kb on + strand, within frmBat 376.909 kb on + strand, within frmBat 376.927 kb on - strand, within frmBat 376.946 kb on - strand, within frmBat 377.044 kb on + strand, within frmBat 377.044 kb on + strand, within frmBat 377.052 kb on - strand, within frmBat 377.075 kb on + strand, within frmBat 377.075 kb on + strand, within frmBat 377.120 kb on - strand, within frmBat 377.120 kb on - strand, within frmBat 377.122 kb on + strand, within frmBat 377.375 kb on - strand, within frmBat 377.395 kb on - strand, within frmBat 377.400 kb on - strand, within frmBat 377.447 kb on + strand, within frmBat 377.499 kb on - strand, within frmBat 377.525 kb on + strandat 377.662 kb on - strandat 377.668 kb on + strandat 377.751 kb on + strandat 377.769 kb on - strandat 377.809 kb on + strand, within frmAat 377.859 kb on - strand, within frmAat 378.034 kb on - strand, within frmAat 378.051 kb on + strand, within frmAat 378.051 kb on + strand, within frmAat 378.135 kb on + strand, within frmAat 378.256 kb on + strand, within frmAat 378.256 kb on + strand, within frmAat 378.256 kb on + strand, within frmAat 378.283 kb on - strand, within frmAat 378.383 kb on - strand, within frmAat 378.383 kb on - strand, within frmAat 378.449 kb on + strand, within frmAat 378.479 kb on - strand, within frmAat 378.479 kb on - strand, within frmAat 378.484 kb on - strand, within frmAat 378.523 kb on - strand, within frmA

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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375,780 - -0.1
375,780 - +0.3
375,806 + -1.6
375,898 + +1.0
375,920 - +0.5
375,921 - +0.7
375,921 - -0.2
375,940 - -0.7
375,940 - +0.3
376,196 + yaiL b0354 0.37 +3.4
376,196 + yaiL b0354 0.37 +1.3
376,199 + yaiL b0354 0.38 -1.1
376,199 + yaiL b0354 0.38 -0.7
376,312 + yaiL b0354 0.59 +0.0
376,331 + yaiL b0354 0.62 -0.5
376,331 + yaiL b0354 0.62 +0.9
376,331 + yaiL b0354 0.62 +1.9
376,336 - yaiL b0354 0.63 -0.8
376,336 - yaiL b0354 0.63 +0.1
376,366 - yaiL b0354 0.69 -0.7
376,366 - yaiL b0354 0.69 +0.3
376,376 - yaiL b0354 0.70 +1.4
376,376 - yaiL b0354 0.70 +1.4
376,384 - yaiL b0354 0.72 +0.3
376,406 - yaiL b0354 0.76 +1.3
376,425 + yaiL b0354 0.79 +1.7
376,449 + yaiL b0354 0.84 +1.3
376,449 - yaiL b0354 0.84 +2.7
376,457 - yaiL b0354 0.85 -0.5
376,481 + yaiL b0354 0.90 -2.4
376,489 - +0.1
376,489 - -0.5
376,489 - +0.0
376,539 + +0.2
376,539 - -0.7
376,558 + -0.9
376,618 - -0.7
376,653 + +0.3
376,682 + -1.9
376,684 + -0.5
376,684 + -0.5
376,692 - +1.6
376,719 + +1.3
376,745 - -0.5
376,745 - -1.2
376,747 + +1.3
376,755 + -0.7
376,771 - +2.6
376,783 + -0.1
376,853 - frmB b0355 0.11 +0.9
376,875 + frmB b0355 0.14 -0.8
376,906 + frmB b0355 0.18 -0.1
376,909 + frmB b0355 0.18 -2.3
376,909 + frmB b0355 0.18 +2.5
376,909 + frmB b0355 0.18 -1.3
376,927 - frmB b0355 0.20 -1.1
376,946 - frmB b0355 0.22 +0.2
377,044 + frmB b0355 0.34 -1.3
377,044 + frmB b0355 0.34 -1.7
377,052 - frmB b0355 0.35 -0.3
377,075 + frmB b0355 0.38 +0.5
377,075 + frmB b0355 0.38 -0.3
377,120 - frmB b0355 0.43 -0.7
377,120 - frmB b0355 0.43 +0.9
377,122 + frmB b0355 0.44 +0.9
377,375 - frmB b0355 0.74 -1.0
377,395 - frmB b0355 0.76 +0.8
377,400 - frmB b0355 0.77 -1.7
377,447 + frmB b0355 0.82 -1.3
377,499 - frmB b0355 0.89 -1.1
377,525 + -0.3
377,662 - +1.2
377,668 + -0.7
377,751 + +0.6
377,769 - +0.6
377,809 + frmA b0356 0.11 +5.4
377,859 - frmA b0356 0.16 -0.9
378,034 - frmA b0356 0.31 -2.7
378,051 + frmA b0356 0.33 -1.1
378,051 + frmA b0356 0.33 +1.3
378,135 + frmA b0356 0.40 +0.8
378,256 + frmA b0356 0.51 +3.4
378,256 + frmA b0356 0.51 -0.9
378,256 + frmA b0356 0.51 -2.3
378,283 - frmA b0356 0.54 -0.8
378,383 - frmA b0356 0.63 -1.4
378,383 - frmA b0356 0.63 -1.1
378,449 + frmA b0356 0.69 -0.1
378,479 - frmA b0356 0.71 -2.1
378,479 - frmA b0356 0.71 -0.8
378,484 - frmA b0356 0.72 -0.7
378,523 - frmA b0356 0.75 +1.0

Or see this region's nucleotide sequence