Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahI and yahJ are separated by 9 nucleotides yahJ and yahK are separated by 376 nucleotides
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
b0324: yahJ - deaminase (NCBI), at 340,349 to 341,731
yahJ
b0325: yahK - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 342,108 to 343,157
yahK
Position (kb)
340
341
342 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI at 340.025 kb on + strand, within yahI at 340.025 kb on + strand, within yahI at 340.047 kb on - strand, within yahI at 340.170 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.257 kb on + strand at 340.257 kb on + strand at 340.320 kb on + strand at 340.320 kb on + strand at 340.394 kb on + strand at 340.448 kb on + strand at 340.461 kb on + strand at 340.522 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.776 kb on + strand, within yahJ at 340.952 kb on + strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.124 kb on + strand, within yahJ at 341.139 kb on - strand, within yahJ at 341.141 kb on + strand, within yahJ at 341.385 kb on - strand, within yahJ at 341.385 kb on - strand, within yahJ at 341.530 kb on + strand, within yahJ at 341.530 kb on + strand, within yahJ at 341.632 kb on + strand at 341.649 kb on - strand at 341.708 kb on + strand at 341.983 kb on - strand at 341.997 kb on + strand at 341.997 kb on - strand at 342.031 kb on + strand at 342.082 kb on - strand at 342.086 kb on - strand at 342.091 kb on + strand at 342.091 kb on + strand at 342.136 kb on - strand at 342.169 kb on + strand at 342.219 kb on + strand, within yahK at 342.219 kb on + strand, within yahK at 342.257 kb on - strand, within yahK at 342.276 kb on - strand, within yahK at 342.322 kb on - strand, within yahK at 342.347 kb on + strand, within yahK at 342.361 kb on + strand, within yahK at 342.422 kb on + strand, within yahK at 342.445 kb on + strand, within yahK at 342.488 kb on - strand, within yahK at 342.493 kb on + strand, within yahK at 342.510 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.525 kb on + strand, within yahK at 342.714 kb on + strand, within yahK at 342.714 kb on + strand, within yahK
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 339,378 - -1.1 339,390 - +1.0 339,390 - +0.3 339,411 - +1.7 339,431 + -0.7 339,444 - +0.6 339,565 + yahI b0323 0.19 -0.3 339,565 + yahI b0323 0.19 -1.3 339,630 + yahI b0323 0.25 +3.0 339,630 + yahI b0323 0.25 -1.9 339,741 - yahI b0323 0.37 +0.7 339,798 + yahI b0323 0.43 -1.2 339,806 - yahI b0323 0.44 +0.7 340,025 + yahI b0323 0.67 +2.0 340,025 + yahI b0323 0.67 -1.9 340,047 - yahI b0323 0.69 +0.5 340,170 - yahI b0323 0.82 +0.0 340,174 - yahI b0323 0.83 +1.5 340,174 - yahI b0323 0.83 +3.8 340,257 + -1.3 340,257 + -0.3 340,320 + +1.3 340,320 + -0.3 340,394 + +1.4 340,448 + -0.7 340,461 + +0.1 340,522 - yahJ b0324 0.13 +0.4 340,573 - yahJ b0324 0.16 -1.1 340,573 - yahJ b0324 0.16 +0.7 340,675 + yahJ b0324 0.24 +1.8 340,675 + yahJ b0324 0.24 +2.2 340,776 + yahJ b0324 0.31 +0.5 340,952 + yahJ b0324 0.44 +1.3 341,087 - yahJ b0324 0.53 -1.0 341,087 - yahJ b0324 0.53 -0.2 341,124 + yahJ b0324 0.56 +1.6 341,139 - yahJ b0324 0.57 +2.5 341,141 + yahJ b0324 0.57 +2.3 341,385 - yahJ b0324 0.75 +1.0 341,385 - yahJ b0324 0.75 +0.2 341,530 + yahJ b0324 0.85 -0.3 341,530 + yahJ b0324 0.85 +0.7 341,632 + -1.5 341,649 - +1.3 341,708 + -1.3 341,983 - +1.0 341,997 + -1.0 341,997 - +1.8 342,031 + -0.5 342,082 - -1.1 342,086 - +1.4 342,091 + +1.7 342,091 + +1.3 342,136 - +1.7 342,169 + -1.9 342,219 + yahK b0325 0.11 +0.7 342,219 + yahK b0325 0.11 -1.5 342,257 - yahK b0325 0.14 -0.6 342,276 - yahK b0325 0.16 +1.7 342,322 - yahK b0325 0.20 +1.1 342,347 + yahK b0325 0.23 -0.6 342,361 + yahK b0325 0.24 -0.3 342,422 + yahK b0325 0.30 +1.0 342,445 + yahK b0325 0.32 +1.0 342,488 - yahK b0325 0.36 -1.5 342,493 + yahK b0325 0.37 -1.3 342,510 + yahK b0325 0.38 +1.6 342,525 + yahK b0325 0.40 +4.1 342,525 + yahK b0325 0.40 +1.0 342,714 + yahK b0325 0.58 -1.3 342,714 + yahK b0325 0.58 -0.7
Or see this region's nucleotide sequence