Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahG and yahI are separated by 421 nucleotides yahI and yahJ are separated by 9 nucleotides
b0321: yahG - hypothetical protein (NCBI), at 337,549 to 338,967
yahG
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
b0324: yahJ - deaminase (NCBI), at 340,349 to 341,731
yahJ
Position (kb)
339
340
341 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 338.424 kb on - strand, within yahG at 338.429 kb on - strand, within yahG at 338.551 kb on - strand, within yahG at 338.656 kb on - strand, within yahG at 338.902 kb on + strand at 338.902 kb on + strand at 339.006 kb on - strand at 339.184 kb on - strand at 339.195 kb on + strand at 339.195 kb on + strand at 339.203 kb on - strand at 339.203 kb on - strand at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI at 340.025 kb on + strand, within yahI at 340.025 kb on + strand, within yahI at 340.047 kb on - strand, within yahI at 340.170 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.257 kb on + strand at 340.257 kb on + strand at 340.320 kb on + strand at 340.320 kb on + strand at 340.394 kb on + strand at 340.448 kb on + strand at 340.461 kb on + strand at 340.522 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.776 kb on + strand, within yahJ at 340.952 kb on + strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.124 kb on + strand, within yahJ at 341.139 kb on - strand, within yahJ at 341.141 kb on + strand, within yahJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 338,424 - yahG b0321 0.62 -1.4 338,429 - yahG b0321 0.62 -0.2 338,551 - yahG b0321 0.71 +3.1 338,656 - yahG b0321 0.78 -0.6 338,902 + -1.1 338,902 + -1.1 339,006 - -1.3 339,184 - -0.2 339,195 + -0.4 339,195 + -0.6 339,203 - +3.2 339,203 - +1.7 339,378 - -1.1 339,390 - +1.0 339,390 - +0.3 339,411 - +1.7 339,431 + -0.7 339,444 - +0.6 339,565 + yahI b0323 0.19 -0.3 339,565 + yahI b0323 0.19 -1.3 339,630 + yahI b0323 0.25 +3.0 339,630 + yahI b0323 0.25 -1.9 339,741 - yahI b0323 0.37 +0.7 339,798 + yahI b0323 0.43 -1.2 339,806 - yahI b0323 0.44 +0.7 340,025 + yahI b0323 0.67 +2.0 340,025 + yahI b0323 0.67 -1.9 340,047 - yahI b0323 0.69 +0.5 340,170 - yahI b0323 0.82 +0.0 340,174 - yahI b0323 0.83 +1.5 340,174 - yahI b0323 0.83 +3.8 340,257 + -1.3 340,257 + -0.3 340,320 + +1.3 340,320 + -0.3 340,394 + +1.4 340,448 + -0.7 340,461 + +0.1 340,522 - yahJ b0324 0.13 +0.4 340,573 - yahJ b0324 0.16 -1.1 340,573 - yahJ b0324 0.16 +0.7 340,675 + yahJ b0324 0.24 +1.8 340,675 + yahJ b0324 0.24 +2.2 340,776 + yahJ b0324 0.31 +0.5 340,952 + yahJ b0324 0.44 +1.3 341,087 - yahJ b0324 0.53 -1.0 341,087 - yahJ b0324 0.53 -0.2 341,124 + yahJ b0324 0.56 +1.6 341,139 - yahJ b0324 0.57 +2.5 341,141 + yahJ b0324 0.57 +2.3
Or see this region's nucleotide sequence