Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt afuC and afuB are separated by 11 nucleotides afuB and insB-2 are separated by 16 nucleotides insB-2 and insA-2 overlap by 82 nucleotides insA-2 and insX are separated by 238 nucleotides
b0262: afuC - putative ATP-binding component of a transport system (VIMSS), at 276,980 to 278,026
afuC
b0263: afuB - CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq), at 278,038 to 278,385
afuB
b0264: insB-2 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 278,402 to 278,905
insB-2
b0265: insA-2 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 278,824 to 279,099
insA-2
b4505: insX - predicted IS protein (RefSeq), at 279,338 to 279,586
insX
Position (kb) 278
279 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 277.206 kb on - strand, within afuC at 277.208 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.296 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.410 kb on + strand, within afuC at 277.410 kb on + strand, within afuC at 277.427 kb on + strand, within afuC at 277.572 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.682 kb on + strand, within afuC at 277.785 kb on - strand, within afuC at 277.787 kb on + strand, within afuC at 277.801 kb on + strand, within afuC at 277.804 kb on - strand, within afuC at 277.804 kb on - strand, within afuC at 277.809 kb on - strand, within afuC at 277.809 kb on - strand, within afuC at 277.992 kb on + strand at 278.030 kb on - strand at 278.187 kb on - strand, within afuB at 278.245 kb on + strand, within afuB at 278.288 kb on + strand, within afuB at 278.350 kb on + strand, within afuB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 277,206 - afuC b0262 0.22 +2.3 277,208 + afuC b0262 0.22 -2.3 277,225 + afuC b0262 0.23 -0.3 277,225 + afuC b0262 0.23 +0.7 277,225 + afuC b0262 0.23 -0.7 277,225 + afuC b0262 0.23 -1.5 277,225 + afuC b0262 0.23 +1.3 277,296 - afuC b0262 0.30 -1.5 277,311 - afuC b0262 0.32 +0.3 277,311 - afuC b0262 0.32 +1.3 277,410 + afuC b0262 0.41 -0.7 277,410 + afuC b0262 0.41 -2.9 277,427 + afuC b0262 0.43 +1.8 277,572 - afuC b0262 0.57 +1.3 277,619 - afuC b0262 0.61 -1.0 277,619 - afuC b0262 0.61 -1.0 277,682 + afuC b0262 0.67 -0.0 277,785 - afuC b0262 0.77 +0.6 277,787 + afuC b0262 0.77 -2.5 277,801 + afuC b0262 0.78 +1.7 277,804 - afuC b0262 0.79 -0.5 277,804 - afuC b0262 0.79 -2.0 277,809 - afuC b0262 0.79 +1.3 277,809 - afuC b0262 0.79 +0.4 277,992 + +1.5 278,030 - -0.7 278,187 - afuB b0263 0.43 -2.4 278,245 + afuB b0263 0.59 -1.0 278,288 + afuB b0263 0.72 -1.3 278,350 + afuB b0263 0.90 +0.6
Or see this region's nucleotide sequence