Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafO and yafP are separated by 9 nucleotides yafP and ykfJ are separated by 317 nucleotides ykfJ and prfH are separated by 16 nucleotides prfH and pepD are separated by 56 nucleotides
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
b0235: ykfJ - hypothetical protein (RefSeq), at 253,479 to 253,685
ykfJ
b0236: prfH - peptide chain release factor 2 (NCBI), at 253,702 to 254,202
prfH
b0237: pepD - aminoacyl-histidine dipeptidase (peptidase D) (NCBI), at 254,259 to 255,716
pepD
Position (kb) 253
254 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP at 252.973 kb on + strand, within yafP at 252.974 kb on + strand, within yafP at 253.041 kb on + strand, within yafP at 253.077 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.102 kb on + strand, within yafP at 253.188 kb on + strand at 253.198 kb on - strand at 253.220 kb on + strand at 253.222 kb on + strand at 253.222 kb on - strand at 253.272 kb on - strand at 253.275 kb on + strand at 253.283 kb on - strand at 253.301 kb on + strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.376 kb on - strand at 253.381 kb on - strand at 253.383 kb on - strand at 253.386 kb on + strand at 253.387 kb on + strand at 253.394 kb on + strand at 253.396 kb on - strand at 253.422 kb on - strand at 253.422 kb on - strand at 253.423 kb on - strand at 253.423 kb on - strand at 253.427 kb on - strand at 253.428 kb on - strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.432 kb on - strand at 253.432 kb on - strand at 253.434 kb on + strand at 253.437 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.450 kb on - strand at 253.454 kb on - strand at 253.491 kb on + strand at 253.504 kb on - strand, within ykfJ at 253.504 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.523 kb on + strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.557 kb on - strand, within ykfJ at 253.569 kb on - strand, within ykfJ at 253.571 kb on - strand, within ykfJ at 253.593 kb on + strand, within ykfJ at 253.603 kb on + strand, within ykfJ at 253.608 kb on - strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.633 kb on - strand, within ykfJ at 253.637 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.681 kb on + strand at 253.727 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.734 kb on - strand at 253.734 kb on - strand at 253.735 kb on - strand at 253.735 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.747 kb on + strand at 253.765 kb on + strand, within prfH at 253.773 kb on + strand, within prfH at 253.805 kb on - strand, within prfH at 253.805 kb on - strand, within prfH at 253.838 kb on - strand, within prfH at 253.891 kb on - strand, within prfH at 254.167 kb on - strand at 254.336 kb on + strand at 254.354 kb on - strand at 254.354 kb on - strand at 254.354 kb on - strand at 254.517 kb on - strand, within pepD at 254.592 kb on - strand, within pepD at 254.593 kb on + strand, within pepD at 254.632 kb on - strand, within pepD at 254.632 kb on - strand, within pepD
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 252,522 - yafO b0233 0.55 +1.3 252,522 - yafO b0233 0.55 +0.6 252,534 - yafO b0233 0.58 -0.4 252,538 + yafO b0233 0.59 +0.3 252,538 + yafO b0233 0.59 +0.3 252,563 + yafO b0233 0.66 -2.9 252,566 - yafO b0233 0.66 -1.9 252,574 - yafO b0233 0.68 -0.3 252,574 - yafO b0233 0.68 +0.9 252,592 - yafO b0233 0.73 -0.5 252,597 + yafO b0233 0.74 -0.3 252,610 - yafO b0233 0.77 +3.2 252,614 + yafO b0233 0.78 -0.3 252,622 - yafO b0233 0.80 +1.2 252,628 + yafO b0233 0.82 -0.6 252,671 + -1.7 252,671 + -1.0 252,739 - -1.3 252,779 - yafP b0234 0.15 -1.5 252,781 + yafP b0234 0.16 +1.5 252,848 + yafP b0234 0.31 -1.0 252,849 + yafP b0234 0.31 -1.5 252,879 + yafP b0234 0.38 +0.3 252,896 + yafP b0234 0.41 -1.0 252,936 + yafP b0234 0.50 -0.8 252,973 + yafP b0234 0.58 -0.9 252,974 + yafP b0234 0.58 -1.2 253,041 + yafP b0234 0.73 -0.8 253,077 + yafP b0234 0.81 +3.8 253,087 + yafP b0234 0.83 -2.3 253,087 + yafP b0234 0.83 -0.5 253,087 + yafP b0234 0.83 -0.0 253,102 + yafP b0234 0.87 -1.5 253,188 + +1.3 253,198 - -2.6 253,220 + -0.7 253,222 + -1.5 253,222 - +0.5 253,272 - -2.4 253,275 + +0.6 253,283 - -0.0 253,301 + -0.6 253,350 - +2.2 253,350 - -0.9 253,350 - +0.7 253,350 - +2.1 253,376 - +0.8 253,381 - +2.3 253,383 - -0.3 253,386 + +2.5 253,387 + -1.9 253,394 + +0.5 253,396 - +0.7 253,422 - +2.7 253,422 - -1.3 253,423 - -0.7 253,423 - +1.5 253,427 - +3.0 253,428 - +0.6 253,430 + -1.0 253,430 + -1.3 253,430 + -0.4 253,430 + -1.6 253,432 - +0.2 253,432 - -2.6 253,434 + +1.3 253,437 - +0.9 253,438 - -1.3 253,438 - +1.7 253,438 - -0.2 253,438 - +0.8 253,450 - -0.9 253,454 - +1.3 253,491 + -0.0 253,504 - ykfJ b0235 0.12 +2.3 253,504 - ykfJ b0235 0.12 -0.4 253,520 - ykfJ b0235 0.20 +2.1 253,520 - ykfJ b0235 0.20 +1.7 253,520 - ykfJ b0235 0.20 -0.3 253,523 + ykfJ b0235 0.21 +1.9 253,533 - ykfJ b0235 0.26 -2.0 253,533 - ykfJ b0235 0.26 +2.7 253,533 - ykfJ b0235 0.26 +1.7 253,533 - ykfJ b0235 0.26 +0.3 253,535 + ykfJ b0235 0.27 +1.0 253,543 - ykfJ b0235 0.31 +1.4 253,543 - ykfJ b0235 0.31 +1.6 253,543 - ykfJ b0235 0.31 -2.0 253,543 - ykfJ b0235 0.31 -1.3 253,543 - ykfJ b0235 0.31 +1.6 253,543 - ykfJ b0235 0.31 -1.2 253,543 - ykfJ b0235 0.31 +0.7 253,543 - ykfJ b0235 0.31 +0.5 253,557 - ykfJ b0235 0.38 -2.0 253,569 - ykfJ b0235 0.43 +1.0 253,571 - ykfJ b0235 0.44 -2.1 253,593 + ykfJ b0235 0.55 -0.0 253,603 + ykfJ b0235 0.60 +0.4 253,608 - ykfJ b0235 0.62 -0.5 253,617 + ykfJ b0235 0.67 -0.6 253,617 + ykfJ b0235 0.67 +3.0 253,617 + ykfJ b0235 0.67 +1.8 253,617 + ykfJ b0235 0.67 -2.0 253,625 - ykfJ b0235 0.71 -0.0 253,625 - ykfJ b0235 0.71 -0.3 253,625 - ykfJ b0235 0.71 -1.7 253,633 - ykfJ b0235 0.74 +0.3 253,637 - ykfJ b0235 0.76 -0.3 253,642 - ykfJ b0235 0.79 -0.4 253,642 - ykfJ b0235 0.79 -0.7 253,642 - ykfJ b0235 0.79 -0.7 253,681 + -1.0 253,727 + -0.0 253,728 + +1.0 253,728 + +2.1 253,728 + -0.6 253,734 - -2.0 253,734 - -0.0 253,735 - +1.3 253,735 - +0.1 253,736 - -0.7 253,736 - -0.7 253,736 - +0.8 253,747 + -2.7 253,765 + prfH b0236 0.13 -0.3 253,773 + prfH b0236 0.14 +0.5 253,805 - prfH b0236 0.21 -0.4 253,805 - prfH b0236 0.21 +1.0 253,838 - prfH b0236 0.27 -0.4 253,891 - prfH b0236 0.38 +0.6 254,167 - -0.2 254,336 + -1.0 254,354 - +1.0 254,354 - -1.6 254,354 - -0.9 254,517 - pepD b0237 0.18 +4.5 254,592 - pepD b0237 0.23 -0.4 254,593 + pepD b0237 0.23 -1.9 254,632 - pepD b0237 0.26 -1.5 254,632 - pepD b0237 0.26 +2.1
Or see this region's nucleotide sequence