Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lfhA and mbhA are separated by 1 nucleotides mbhA and dinB are separated by 70 nucleotides dinB and yafN are separated by 51 nucleotides yafN and yafO are separated by 2 nucleotides yafO and yafP are separated by 9 nucleotides
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
b0230: mbhA - putative motility protein (VIMSS), at 250,072 to 250,827
mbhA
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
b0232: yafN - predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,005 to 252,298
yafN
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
Position (kb) 250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 250.046 kb on + strand at 250.049 kb on + strand at 250.049 kb on + strand at 250.295 kb on - strand, within mbhA at 250.295 kb on - strand, within mbhA at 250.355 kb on + strand, within mbhA at 250.374 kb on + strand, within mbhA at 250.389 kb on + strand, within mbhA at 250.426 kb on + strand, within mbhA at 250.442 kb on - strand, within mbhA at 250.473 kb on - strand, within mbhA at 250.501 kb on + strand, within mbhA at 250.559 kb on - strand, within mbhA at 250.594 kb on - strand, within mbhA at 250.697 kb on + strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.729 kb on + strand, within mbhA at 250.959 kb on + strand at 250.988 kb on - strand at 251.129 kb on - strand at 251.129 kb on - strand, within dinB at 251.214 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on - strand, within dinB at 251.240 kb on - strand, within dinB at 251.255 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.579 kb on - strand, within dinB at 251.662 kb on - strand, within dinB at 251.669 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.818 kb on + strand, within dinB at 251.880 kb on + strand at 251.901 kb on + strand at 251.909 kb on - strand at 251.924 kb on + strand at 251.965 kb on - strand at 252.003 kb on - strand at 252.137 kb on - strand, within yafN at 252.155 kb on - strand, within yafN at 252.288 kb on + strand at 252.331 kb on + strand at 252.331 kb on + strand at 252.341 kb on - strand, within yafO at 252.346 kb on + strand, within yafO at 252.368 kb on - strand, within yafO at 252.368 kb on - strand, within yafO at 252.380 kb on + strand, within yafO at 252.381 kb on + strand, within yafO at 252.419 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.443 kb on - strand, within yafO at 252.475 kb on + strand, within yafO at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 250,046 + +1.3 250,049 + +0.5 250,049 + -1.3 250,295 - mbhA b0230 0.29 +0.3 250,295 - mbhA b0230 0.29 +0.6 250,355 + mbhA b0230 0.37 +0.3 250,374 + mbhA b0230 0.40 +2.6 250,389 + mbhA b0230 0.42 +1.3 250,426 + mbhA b0230 0.47 -0.3 250,442 - mbhA b0230 0.49 -2.3 250,473 - mbhA b0230 0.53 +0.6 250,501 + mbhA b0230 0.57 +0.9 250,559 - mbhA b0230 0.64 +3.0 250,594 - mbhA b0230 0.69 -0.8 250,697 + mbhA b0230 0.83 +1.1 250,718 - mbhA b0230 0.85 +4.6 250,718 - mbhA b0230 0.85 -1.3 250,729 + mbhA b0230 0.87 +1.3 250,959 + +2.3 250,988 - -0.3 251,129 - +4.3 251,129 - dinB b0231 0.22 -0.1 251,214 + dinB b0231 0.30 +0.6 251,240 + dinB b0231 0.32 -0.0 251,240 + dinB b0231 0.32 +0.7 251,240 - dinB b0231 0.32 -0.7 251,240 - dinB b0231 0.32 -1.4 251,255 - dinB b0231 0.34 -1.4 251,499 - dinB b0231 0.57 -1.7 251,499 - dinB b0231 0.57 +1.7 251,579 - dinB b0231 0.64 -1.5 251,662 - dinB b0231 0.72 +0.0 251,669 + dinB b0231 0.73 -1.4 251,803 + dinB b0231 0.86 -1.4 251,803 + dinB b0231 0.86 -0.3 251,818 + dinB b0231 0.87 -0.3 251,880 + -0.5 251,901 + -2.5 251,909 - -1.1 251,924 + +0.3 251,965 - +0.3 252,003 - +3.0 252,137 - yafN b0232 0.45 -0.3 252,155 - yafN b0232 0.51 +0.1 252,288 + +0.8 252,331 + -1.5 252,331 + +0.7 252,341 - yafO b0233 0.10 +0.6 252,346 + yafO b0233 0.11 -1.0 252,368 - yafO b0233 0.17 -2.0 252,368 - yafO b0233 0.17 -0.3 252,380 + yafO b0233 0.20 -1.0 252,381 + yafO b0233 0.20 +1.4 252,419 + yafO b0233 0.30 -0.8 252,427 + yafO b0233 0.32 +0.6 252,427 + yafO b0233 0.32 -0.0 252,440 + yafO b0233 0.35 +0.4 252,440 + yafO b0233 0.35 -0.7 252,440 + yafO b0233 0.35 -0.6 252,440 + yafO b0233 0.35 +0.2 252,440 + yafO b0233 0.35 +0.8 252,443 - yafO b0233 0.36 -0.8 252,475 + yafO b0233 0.44 +0.6 252,522 - yafO b0233 0.55 +1.3 252,522 - yafO b0233 0.55 +0.6 252,534 - yafO b0233 0.58 -0.4 252,538 + yafO b0233 0.59 +0.3 252,538 + yafO b0233 0.59 +0.3 252,563 + yafO b0233 0.66 -2.9 252,566 - yafO b0233 0.66 -1.9 252,574 - yafO b0233 0.68 -0.3 252,574 - yafO b0233 0.68 +0.9 252,592 - yafO b0233 0.73 -0.5 252,597 + yafO b0233 0.74 -0.3 252,610 - yafO b0233 0.77 +3.2 252,614 + yafO b0233 0.78 -0.3 252,622 - yafO b0233 0.80 +1.2 252,628 + yafO b0233 0.82 -0.6 252,671 + -1.7 252,671 + -1.0 252,739 - -1.3 252,779 - yafP b0234 0.15 -1.5 252,781 + yafP b0234 0.16 +1.5 252,848 + yafP b0234 0.31 -1.0 252,849 + yafP b0234 0.31 -1.5 252,879 + yafP b0234 0.38 +0.3 252,896 + yafP b0234 0.41 -1.0 252,936 + yafP b0234 0.50 -0.8
Or see this region's nucleotide sequence