Strain Fitness in Escherichia coli BW25113 around b0230

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlfhA and mbhA are separated by 1 nucleotidesmbhA and dinB are separated by 70 nucleotides b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070 lfhA b0230: mbhA - putative motility protein (VIMSS), at 250,072 to 250,827 mbhA b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953 dinB Position (kb) 250 251Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 249.077 kb on + strand, within lfhAat 249.103 kb on - strand, within lfhAat 249.388 kb on - strand, within lfhAat 249.432 kb on + strand, within lfhAat 249.572 kb on - strand, within lfhAat 249.572 kb on - strand, within lfhAat 249.594 kb on - strand, within lfhAat 249.687 kb on - strand, within lfhAat 249.744 kb on + strand, within lfhAat 250.046 kb on + strandat 250.049 kb on + strandat 250.049 kb on + strandat 250.295 kb on - strand, within mbhAat 250.295 kb on - strand, within mbhAat 250.355 kb on + strand, within mbhAat 250.374 kb on + strand, within mbhAat 250.389 kb on + strand, within mbhAat 250.426 kb on + strand, within mbhAat 250.442 kb on - strand, within mbhAat 250.473 kb on - strand, within mbhAat 250.501 kb on + strand, within mbhAat 250.559 kb on - strand, within mbhAat 250.594 kb on - strand, within mbhAat 250.697 kb on + strand, within mbhAat 250.718 kb on - strand, within mbhAat 250.718 kb on - strand, within mbhAat 250.729 kb on + strand, within mbhAat 250.959 kb on + strandat 250.988 kb on - strandat 251.129 kb on - strandat 251.129 kb on - strand, within dinBat 251.214 kb on + strand, within dinBat 251.240 kb on + strand, within dinBat 251.240 kb on + strand, within dinBat 251.240 kb on - strand, within dinBat 251.240 kb on - strand, within dinBat 251.255 kb on - strand, within dinBat 251.499 kb on - strand, within dinBat 251.499 kb on - strand, within dinBat 251.579 kb on - strand, within dinBat 251.662 kb on - strand, within dinBat 251.669 kb on + strand, within dinBat 251.803 kb on + strand, within dinBat 251.803 kb on + strand, within dinBat 251.818 kb on + strand, within dinB

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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249,077 + lfhA b0229 0.42 +0.6
249,103 - lfhA b0229 0.43 +0.7
249,388 - lfhA b0229 0.60 -0.5
249,432 + lfhA b0229 0.63 +1.6
249,572 - lfhA b0229 0.71 +0.6
249,572 - lfhA b0229 0.71 +1.6
249,594 - lfhA b0229 0.72 +2.2
249,687 - lfhA b0229 0.78 -1.0
249,744 + lfhA b0229 0.81 -0.4
250,046 + +1.3
250,049 + +0.5
250,049 + -1.3
250,295 - mbhA b0230 0.29 +0.3
250,295 - mbhA b0230 0.29 +0.6
250,355 + mbhA b0230 0.37 +0.3
250,374 + mbhA b0230 0.40 +2.6
250,389 + mbhA b0230 0.42 +1.3
250,426 + mbhA b0230 0.47 -0.3
250,442 - mbhA b0230 0.49 -2.3
250,473 - mbhA b0230 0.53 +0.6
250,501 + mbhA b0230 0.57 +0.9
250,559 - mbhA b0230 0.64 +3.0
250,594 - mbhA b0230 0.69 -0.8
250,697 + mbhA b0230 0.83 +1.1
250,718 - mbhA b0230 0.85 +4.6
250,718 - mbhA b0230 0.85 -1.3
250,729 + mbhA b0230 0.87 +1.3
250,959 + +2.3
250,988 - -0.3
251,129 - +4.3
251,129 - dinB b0231 0.22 -0.1
251,214 + dinB b0231 0.30 +0.6
251,240 + dinB b0231 0.32 -0.0
251,240 + dinB b0231 0.32 +0.7
251,240 - dinB b0231 0.32 -0.7
251,240 - dinB b0231 0.32 -1.4
251,255 - dinB b0231 0.34 -1.4
251,499 - dinB b0231 0.57 -1.7
251,499 - dinB b0231 0.57 +1.7
251,579 - dinB b0231 0.64 -1.5
251,662 - dinB b0231 0.72 +0.0
251,669 + dinB b0231 0.73 -1.4
251,803 + dinB b0231 0.86 -1.4
251,803 + dinB b0231 0.86 -0.3
251,818 + dinB b0231 0.87 -0.3

Or see this region's nucleotide sequence