Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafD and yafE are separated by 3 nucleotides yafE and mltD are separated by 47 nucleotides mltD and gloB are separated by 71 nucleotides gloB and yafS are separated by 33 nucleotides
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
b0212: gloB - predicted hydroxyacylglutathione hydrolase (NCBI), at 234,027 to 234,782
gloB
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
Position (kb) 232
233
234 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand at 231.935 kb on - strand at 231.935 kb on - strand at 231.938 kb on + strand at 231.946 kb on - strand at 231.970 kb on + strand at 231.983 kb on + strand at 231.983 kb on + strand at 232.010 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.303 kb on + strand, within yafE at 232.469 kb on + strand, within yafE at 232.656 kb on + strand at 232.678 kb on - strand at 232.728 kb on - strand at 232.728 kb on - strand at 232.760 kb on - strand, within mltD at 232.830 kb on + strand, within mltD at 232.830 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.923 kb on - strand, within mltD at 232.923 kb on - strand, within mltD at 233.026 kb on - strand, within mltD at 233.047 kb on - strand, within mltD at 233.065 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.156 kb on + strand, within mltD at 233.376 kb on - strand, within mltD at 233.376 kb on - strand, within mltD at 233.472 kb on + strand, within mltD at 233.472 kb on + strand, within mltD at 233.498 kb on - strand, within mltD at 233.697 kb on - strand, within mltD at 233.698 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.774 kb on + strand, within mltD at 233.774 kb on + strand, within mltD at 233.875 kb on + strand at 233.914 kb on + strand at 234.054 kb on - strand at 234.253 kb on - strand, within gloB at 234.292 kb on + strand, within gloB at 234.292 kb on + strand, within gloB at 234.386 kb on + strand, within gloB at 234.386 kb on - strand, within gloB at 234.556 kb on - strand, within gloB at 234.737 kb on - strand at 234.737 kb on - strand at 234.796 kb on + strand at 234.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 231,601 + +1.3 231,621 + yafD b0209 0.62 -1.4 231,627 - yafD b0209 0.63 -1.6 231,627 - yafD b0209 0.63 +3.3 231,659 + yafD b0209 0.67 +0.7 231,659 + yafD b0209 0.67 +1.3 231,664 - yafD b0209 0.68 -0.7 231,664 - yafD b0209 0.68 +0.5 231,667 - yafD b0209 0.68 +1.1 231,755 + yafD b0209 0.79 +2.1 231,828 + yafD b0209 0.88 +1.3 231,828 + yafD b0209 0.88 -0.3 231,837 + yafD b0209 0.89 +0.1 231,851 + -1.3 231,851 + +0.5 231,935 - -1.3 231,935 - +2.8 231,938 + -0.1 231,946 - +0.2 231,970 + -1.0 231,983 + +2.8 231,983 + +0.3 232,010 + yafE b0210 0.13 +0.6 232,254 + yafE b0210 0.53 +0.1 232,254 + yafE b0210 0.53 -0.0 232,254 + yafE b0210 0.53 +1.3 232,303 + yafE b0210 0.60 -0.4 232,469 + yafE b0210 0.87 +2.2 232,656 + -0.2 232,678 - +1.2 232,728 - -1.7 232,728 - +0.3 232,760 - mltD b0211 0.12 +1.9 232,830 + mltD b0211 0.17 +1.9 232,830 + mltD b0211 0.17 -0.4 232,839 + mltD b0211 0.18 +1.5 232,839 + mltD b0211 0.18 +0.3 232,923 - mltD b0211 0.24 +2.5 232,923 - mltD b0211 0.24 +1.3 233,026 - mltD b0211 0.32 +0.4 233,047 - mltD b0211 0.33 +1.2 233,065 - mltD b0211 0.34 -1.0 233,075 - mltD b0211 0.35 +1.8 233,075 - mltD b0211 0.35 +1.5 233,156 + mltD b0211 0.41 +0.5 233,376 - mltD b0211 0.57 -0.3 233,376 - mltD b0211 0.57 -0.3 233,472 + mltD b0211 0.64 +0.5 233,472 + mltD b0211 0.64 -0.3 233,498 - mltD b0211 0.66 +2.7 233,697 - mltD b0211 0.81 -1.4 233,698 - mltD b0211 0.81 +0.2 233,760 - mltD b0211 0.86 -0.8 233,760 - mltD b0211 0.86 -0.7 233,774 + mltD b0211 0.87 -0.3 233,774 + mltD b0211 0.87 +0.3 233,875 + -0.7 233,914 + -0.6 234,054 - -2.2 234,253 - gloB b0212 0.30 +0.1 234,292 + gloB b0212 0.35 -0.7 234,292 + gloB b0212 0.35 -1.0 234,386 + gloB b0212 0.47 -0.7 234,386 - gloB b0212 0.47 +1.9 234,556 - gloB b0212 0.70 -2.2 234,737 - -1.7 234,737 - -1.0 234,796 + +1.3 234,796 + -0.3
Or see this region's nucleotide sequence