Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspU and dkgB are separated by 162 nucleotides dkgB and yafC overlap by 4 nucleotides yafC and yafD are separated by 240 nucleotides
b0206: aspU - tRNA-Asp (NCBI), at 228,928 to 229,004
aspU
b0207: dkgB - 2,5-diketo-D-gluconate reductase B (NCBI), at 229,167 to 229,970
dkgB
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
Position (kb) 229
230
231 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 229.157 kb on - strand at 229.224 kb on - strand at 229.230 kb on - strand at 229.351 kb on - strand, within dkgB at 229.437 kb on - strand, within dkgB at 229.491 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.685 kb on + strand, within dkgB at 229.755 kb on + strand, within dkgB at 229.763 kb on - strand, within dkgB at 229.768 kb on - strand, within dkgB at 229.798 kb on + strand, within dkgB at 229.802 kb on + strand, within dkgB at 229.803 kb on + strand, within dkgB at 229.830 kb on + strand, within dkgB at 229.972 kb on - strand at 229.972 kb on - strand at 230.069 kb on - strand, within yafC at 230.208 kb on - strand, within yafC at 230.223 kb on - strand, within yafC at 230.230 kb on + strand, within yafC at 230.244 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.282 kb on + strand, within yafC at 230.282 kb on + strand, within yafC at 230.323 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.572 kb on + strand, within yafC at 230.600 kb on - strand, within yafC at 230.611 kb on + strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 231.017 kb on - strand at 231.145 kb on + strand at 231.161 kb on - strand at 231.161 kb on - strand at 231.183 kb on - strand at 231.235 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.535 kb on + strand, within yafD at 231.583 kb on - strand, within yafD at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 229,157 - -1.2 229,224 - +2.3 229,230 - -0.4 229,351 - dkgB b0207 0.23 +0.2 229,437 - dkgB b0207 0.34 +4.1 229,491 - dkgB b0207 0.40 -1.7 229,501 - dkgB b0207 0.42 +0.3 229,501 - dkgB b0207 0.42 -1.5 229,674 - dkgB b0207 0.63 -0.8 229,674 - dkgB b0207 0.63 +2.0 229,685 + dkgB b0207 0.64 -1.2 229,755 + dkgB b0207 0.73 +2.2 229,763 - dkgB b0207 0.74 +1.0 229,768 - dkgB b0207 0.75 +0.4 229,798 + dkgB b0207 0.78 -1.9 229,802 + dkgB b0207 0.79 -1.1 229,803 + dkgB b0207 0.79 -1.0 229,830 + dkgB b0207 0.82 -1.5 229,972 - +0.7 229,972 - +0.6 230,069 - yafC b0208 0.11 +1.5 230,208 - yafC b0208 0.26 +0.6 230,223 - yafC b0208 0.28 +0.9 230,230 + yafC b0208 0.29 -1.6 230,244 - yafC b0208 0.30 +1.2 230,276 - yafC b0208 0.34 -1.5 230,276 - yafC b0208 0.34 +0.1 230,282 + yafC b0208 0.34 +2.0 230,282 + yafC b0208 0.34 -1.0 230,323 - yafC b0208 0.39 -2.2 230,549 - yafC b0208 0.64 +3.0 230,549 - yafC b0208 0.64 +2.8 230,572 + yafC b0208 0.66 -1.9 230,600 - yafC b0208 0.69 +0.9 230,611 + yafC b0208 0.70 +0.5 230,732 - yafC b0208 0.84 +0.1 230,732 - yafC b0208 0.84 -0.5 230,732 - yafC b0208 0.84 +1.2 230,785 - yafC b0208 0.89 +1.6 230,785 - yafC b0208 0.89 +2.1 231,017 - +2.1 231,145 + +0.2 231,161 - +0.3 231,161 - -1.0 231,183 - +0.3 231,235 + yafD b0209 0.14 -0.7 231,446 + yafD b0209 0.40 +0.7 231,446 + yafD b0209 0.40 -1.2 231,535 + yafD b0209 0.52 -0.3 231,583 - yafD b0209 0.58 +1.6 231,601 + +1.3 231,621 + yafD b0209 0.62 -1.4 231,627 - yafD b0209 0.63 -1.6 231,627 - yafD b0209 0.63 +3.3 231,659 + yafD b0209 0.67 +0.7 231,659 + yafD b0209 0.67 +1.3 231,664 - yafD b0209 0.68 -0.7 231,664 - yafD b0209 0.68 +0.5 231,667 - yafD b0209 0.68 +1.1 231,755 + yafD b0209 0.79 +2.1 231,828 + yafD b0209 0.88 +1.3 231,828 + yafD b0209 0.88 -0.3 231,837 + yafD b0209 0.89 +0.1 231,851 + -1.3 231,851 + +0.5
Or see this region's nucleotide sequence