Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeQ and yaeJ overlap by 4 nucleotides yaeJ and nlpE are separated by 13 nucleotides nlpE and yaeF are separated by 199 nucleotides
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
Position (kb) 215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.897 kb on - strand, within nlpE at 215.915 kb on - strand at 215.915 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on - strand at 215.933 kb on - strand at 215.958 kb on + strand at 215.968 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand at 216.282 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.326 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.417 kb on + strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.444 kb on + strand, within yaeF at 216.505 kb on + strand, within yaeF at 216.635 kb on + strand, within yaeF at 216.733 kb on + strand, within yaeF at 216.749 kb on - strand, within yaeF at 216.774 kb on - strand, within yaeF at 216.786 kb on - strand, within yaeF at 216.804 kb on + strand, within yaeF at 216.868 kb on - strand, within yaeF at 216.896 kb on + strand, within yaeF at 216.896 kb on + strand, within yaeF
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 214,369 - yaeQ b0190 0.14 -2.0 214,391 - yaeQ b0190 0.18 -1.7 214,404 - yaeQ b0190 0.21 -1.0 214,419 - yaeQ b0190 0.23 -1.1 214,468 + yaeQ b0190 0.32 -0.3 214,487 + yaeQ b0190 0.36 +1.1 214,487 + yaeQ b0190 0.36 -1.1 214,542 + yaeQ b0190 0.46 +1.1 214,572 + yaeQ b0190 0.51 -0.6 214,574 + yaeQ b0190 0.52 +0.1 214,600 - yaeQ b0190 0.57 +1.3 214,606 + yaeQ b0190 0.58 +2.7 214,606 + yaeQ b0190 0.58 +3.3 214,764 - yaeQ b0190 0.87 +0.7 214,773 - yaeQ b0190 0.88 -0.5 214,808 + +3.1 214,825 - -0.9 214,836 - -1.9 214,872 + +2.8 214,874 + +0.3 214,919 + yaeJ b0191 0.20 +1.2 215,045 + yaeJ b0191 0.50 +2.2 215,045 + yaeJ b0191 0.50 +1.3 215,091 + yaeJ b0191 0.61 +0.9 215,123 + yaeJ b0191 0.69 +0.8 215,123 + yaeJ b0191 0.69 +0.9 215,299 + -1.7 215,372 + nlpE b0192 0.14 +1.0 215,378 + nlpE b0192 0.15 +1.0 215,410 + nlpE b0192 0.20 +0.0 215,476 - nlpE b0192 0.29 +2.4 215,498 - nlpE b0192 0.32 -0.7 215,498 - nlpE b0192 0.32 +0.3 215,558 + nlpE b0192 0.41 -0.4 215,582 - nlpE b0192 0.44 -0.6 215,621 + nlpE b0192 0.50 +2.1 215,635 - nlpE b0192 0.51 +0.7 215,635 - nlpE b0192 0.51 -1.0 215,694 + nlpE b0192 0.60 +2.0 215,756 + nlpE b0192 0.68 -1.5 215,816 + nlpE b0192 0.77 +3.4 215,816 + nlpE b0192 0.77 -0.3 215,824 - nlpE b0192 0.78 -1.1 215,864 - nlpE b0192 0.84 +0.5 215,864 - nlpE b0192 0.84 +0.5 215,895 - nlpE b0192 0.88 -0.0 215,895 - nlpE b0192 0.88 +1.8 215,897 - nlpE b0192 0.88 -0.1 215,915 - +1.3 215,915 - +0.3 215,931 - +0.1 215,931 - +0.7 215,933 - +3.2 215,933 - +2.3 215,958 + -0.6 215,968 - +0.2 216,101 - -1.4 216,101 - -0.3 216,101 - -0.4 216,151 + -0.3 216,159 - -0.4 216,159 - -2.0 216,160 - -0.3 216,160 - +0.7 216,235 + -0.3 216,235 + +1.1 216,282 + yaeF b0193 0.12 -1.9 216,285 + yaeF b0193 0.13 -1.0 216,285 + yaeF b0193 0.13 -0.9 216,326 - yaeF b0193 0.18 +1.8 216,332 - yaeF b0193 0.19 +1.0 216,332 - yaeF b0193 0.19 +2.2 216,334 + yaeF b0193 0.19 -0.9 216,334 + yaeF b0193 0.19 -0.3 216,342 - yaeF b0193 0.20 -1.3 216,342 - yaeF b0193 0.20 +0.4 216,417 + yaeF b0193 0.29 +0.3 216,417 - yaeF b0193 0.29 +0.3 216,417 - yaeF b0193 0.29 +2.7 216,444 + yaeF b0193 0.32 +1.0 216,505 + yaeF b0193 0.40 +0.3 216,635 + yaeF b0193 0.55 -1.7 216,733 + yaeF b0193 0.67 +0.0 216,749 - yaeF b0193 0.69 -1.9 216,774 - yaeF b0193 0.72 -0.7 216,786 - yaeF b0193 0.74 +3.2 216,804 + yaeF b0193 0.76 -0.8 216,868 - yaeF b0193 0.84 +2.2 216,896 + yaeF b0193 0.87 -2.2 216,896 + yaeF b0193 0.87 -0.3
Or see this region's nucleotide sequence