Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuA and fhuC are separated by 50 nucleotides fhuC and fhuD overlap by 1 nucleotides fhuD and fhuB overlap by 4 nucleotides
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 168.800 kb on + strand, within fhuA at 168.899 kb on + strand, within fhuA at 168.942 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.944 kb on - strand, within fhuA at 168.961 kb on + strand, within fhuA at 169.064 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.127 kb on + strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.135 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.167 kb on - strand, within fhuA at 169.202 kb on + strand, within fhuA at 169.260 kb on + strand, within fhuA at 169.330 kb on + strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.354 kb on - strand, within fhuA at 169.355 kb on - strand, within fhuA at 169.361 kb on + strand, within fhuA at 169.390 kb on - strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.437 kb on + strand, within fhuA at 169.467 kb on + strand, within fhuA at 169.483 kb on + strand, within fhuA at 169.490 kb on + strand, within fhuA at 169.528 kb on + strand at 169.549 kb on - strand at 169.556 kb on - strand at 169.594 kb on - strand at 169.620 kb on + strand at 169.629 kb on - strand at 169.635 kb on - strand at 169.635 kb on - strand at 169.639 kb on - strand at 169.684 kb on - strand at 169.827 kb on - strand at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.929 kb on - strand, within fhuC at 169.965 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.989 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.250 kb on + strand, within fhuC at 170.250 kb on - strand, within fhuC at 170.255 kb on + strand, within fhuC at 170.257 kb on + strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.395 kb on - strand, within fhuC at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.775 kb on + strand, within fhuD at 170.831 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.972 kb on - strand, within fhuD at 171.065 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.219 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.307 kb on - strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.423 kb on + strand at 171.423 kb on - strand at 171.423 kb on - strand at 171.506 kb on + strand at 171.514 kb on - strand at 171.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 168,800 + fhuA b0150 0.59 +1.1 168,899 + fhuA b0150 0.63 -0.5 168,942 - fhuA b0150 0.65 -1.0 168,944 - fhuA b0150 0.65 -0.7 168,944 - fhuA b0150 0.65 +3.5 168,961 + fhuA b0150 0.66 -1.4 169,064 + fhuA b0150 0.70 -1.3 169,127 + fhuA b0150 0.73 +0.2 169,127 + fhuA b0150 0.73 -1.5 169,135 - fhuA b0150 0.74 -1.9 169,135 - fhuA b0150 0.74 -1.9 169,167 - fhuA b0150 0.75 +0.6 169,167 - fhuA b0150 0.75 +1.6 169,202 + fhuA b0150 0.77 +2.0 169,260 + fhuA b0150 0.79 -0.9 169,330 + fhuA b0150 0.82 +1.8 169,354 - fhuA b0150 0.83 +2.2 169,354 - fhuA b0150 0.83 +0.3 169,355 - fhuA b0150 0.83 +0.1 169,361 + fhuA b0150 0.84 +0.3 169,390 - fhuA b0150 0.85 +1.7 169,437 + fhuA b0150 0.87 -1.3 169,437 + fhuA b0150 0.87 -1.4 169,467 + fhuA b0150 0.88 +0.1 169,483 + fhuA b0150 0.89 +1.5 169,490 + fhuA b0150 0.89 +1.6 169,528 + +1.6 169,549 - +3.1 169,556 - -0.1 169,594 - -1.3 169,620 + -0.7 169,629 - +2.0 169,635 - -1.0 169,635 - +0.3 169,639 - +1.3 169,684 - -1.6 169,827 - +1.3 169,926 + fhuC b0151 0.19 -1.5 169,926 + fhuC b0151 0.19 +1.3 169,926 + fhuC b0151 0.19 -1.8 169,929 - fhuC b0151 0.19 +1.7 169,965 + fhuC b0151 0.23 +1.6 169,966 + fhuC b0151 0.24 +0.3 169,966 + fhuC b0151 0.24 +1.5 169,989 + fhuC b0151 0.26 +1.6 170,210 + fhuC b0151 0.54 +2.6 170,210 + fhuC b0151 0.54 +1.3 170,250 + fhuC b0151 0.59 +0.3 170,250 - fhuC b0151 0.59 +2.3 170,255 + fhuC b0151 0.60 -1.0 170,257 + fhuC b0151 0.60 -2.5 170,390 - fhuC b0151 0.77 -0.0 170,390 - fhuC b0151 0.77 -2.0 170,395 - fhuC b0151 0.77 +1.0 170,654 - +2.0 170,671 + fhuD b0152 0.11 -0.5 170,671 + fhuD b0152 0.11 +0.9 170,689 - fhuD b0152 0.13 -1.6 170,739 + fhuD b0152 0.18 +0.9 170,739 + fhuD b0152 0.18 +0.3 170,755 - fhuD b0152 0.20 +2.6 170,755 - fhuD b0152 0.20 -0.5 170,775 + fhuD b0152 0.22 -0.7 170,831 + fhuD b0152 0.29 +0.3 170,869 + fhuD b0152 0.33 -1.3 170,869 + fhuD b0152 0.33 -2.6 170,895 - fhuD b0152 0.36 +0.8 170,895 - fhuD b0152 0.36 -1.4 170,972 - fhuD b0152 0.45 -0.1 171,065 + fhuD b0152 0.55 +0.6 171,199 + fhuD b0152 0.70 +0.7 171,199 + fhuD b0152 0.70 +2.2 171,219 + fhuD b0152 0.72 +0.6 171,225 + fhuD b0152 0.73 +0.6 171,225 + fhuD b0152 0.73 -0.4 171,228 + fhuD b0152 0.73 +0.1 171,228 + fhuD b0152 0.73 -0.7 171,238 - fhuD b0152 0.74 -1.8 171,238 - fhuD b0152 0.74 -1.5 171,307 - fhuD b0152 0.82 -1.9 171,336 + fhuD b0152 0.85 +0.6 171,336 + fhuD b0152 0.85 -1.0 171,423 + -1.2 171,423 - -0.4 171,423 - -0.3 171,506 + +1.2 171,514 - +1.4 171,533 - +1.8
Or see this region's nucleotide sequence