Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpd and yacH are separated by 70 nucleotides yacH and acnB are separated by 354 nucleotides
b0116: lpd - dihydrolipoamide dehydrogenase (NCBI), at 127,912 to 129,336
lpd
b0117: yacH - hypothetical protein (NCBI), at 129,407 to 131,260
yacH
b0118: acnB - aconitate hydratase (NCBI), at 131,615 to 134,212
acnB
Position (kb) 129
130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 129.309 kb on + strand at 129.309 kb on + strand at 129.429 kb on - strand at 129.521 kb on + strand at 129.521 kb on + strand at 129.617 kb on + strand, within yacH at 129.617 kb on + strand, within yacH at 129.644 kb on + strand, within yacH at 129.644 kb on + strand, within yacH at 129.647 kb on + strand, within yacH at 129.647 kb on + strand, within yacH at 129.649 kb on + strand, within yacH at 129.649 kb on + strand, within yacH at 129.657 kb on - strand, within yacH at 129.670 kb on - strand, within yacH at 129.675 kb on - strand, within yacH at 129.675 kb on - strand, within yacH at 129.692 kb on - strand, within yacH at 129.692 kb on - strand, within yacH at 129.776 kb on + strand, within yacH at 129.778 kb on + strand, within yacH at 129.839 kb on - strand, within yacH at 129.841 kb on + strand, within yacH at 129.841 kb on + strand, within yacH at 129.847 kb on - strand, within yacH at 129.867 kb on - strand, within yacH at 129.936 kb on + strand, within yacH at 130.001 kb on + strand, within yacH at 130.003 kb on + strand, within yacH at 130.041 kb on + strand, within yacH at 130.086 kb on + strand, within yacH at 130.109 kb on + strand, within yacH at 130.109 kb on + strand, within yacH at 130.136 kb on - strand, within yacH at 130.146 kb on - strand, within yacH at 130.151 kb on - strand, within yacH at 130.168 kb on - strand, within yacH at 130.173 kb on - strand, within yacH at 130.173 kb on - strand, within yacH at 130.180 kb on + strand, within yacH at 130.201 kb on - strand, within yacH at 130.272 kb on - strand, within yacH at 130.349 kb on + strand, within yacH at 130.376 kb on + strand, within yacH at 130.376 kb on + strand, within yacH at 130.379 kb on + strand, within yacH at 130.379 kb on + strand, within yacH at 130.382 kb on - strand, within yacH at 130.476 kb on + strand, within yacH at 130.506 kb on + strand, within yacH at 130.521 kb on + strand, within yacH at 130.521 kb on + strand, within yacH at 130.576 kb on + strand, within yacH at 130.595 kb on + strand, within yacH at 130.615 kb on + strand, within yacH at 130.615 kb on + strand, within yacH at 130.649 kb on - strand, within yacH at 130.649 kb on - strand, within yacH at 130.693 kb on + strand, within yacH at 130.732 kb on + strand, within yacH at 130.742 kb on + strand, within yacH at 130.742 kb on + strand, within yacH at 130.796 kb on + strand, within yacH at 130.871 kb on + strand, within yacH at 130.879 kb on - strand, within yacH at 130.930 kb on + strand, within yacH at 131.045 kb on - strand, within yacH at 131.228 kb on - strand at 131.228 kb on - strand at 131.369 kb on + strand at 131.369 kb on + strand at 131.389 kb on - strand at 131.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 129,309 + -0.7 129,309 + -0.7 129,429 - +0.7 129,521 + +2.0 129,521 + -1.0 129,617 + yacH b0117 0.11 +2.3 129,617 + yacH b0117 0.11 -1.4 129,644 + yacH b0117 0.13 +2.3 129,644 + yacH b0117 0.13 -0.3 129,647 + yacH b0117 0.13 +1.0 129,647 + yacH b0117 0.13 -2.1 129,649 + yacH b0117 0.13 +1.3 129,649 + yacH b0117 0.13 +2.3 129,657 - yacH b0117 0.13 +2.1 129,670 - yacH b0117 0.14 +1.0 129,675 - yacH b0117 0.14 -2.9 129,675 - yacH b0117 0.14 +1.3 129,692 - yacH b0117 0.15 -1.0 129,692 - yacH b0117 0.15 +2.4 129,776 + yacH b0117 0.20 +0.3 129,778 + yacH b0117 0.20 +0.7 129,839 - yacH b0117 0.23 +1.9 129,841 + yacH b0117 0.23 -0.4 129,841 + yacH b0117 0.23 -0.8 129,847 - yacH b0117 0.24 -1.7 129,867 - yacH b0117 0.25 +0.9 129,936 + yacH b0117 0.29 -0.4 130,001 + yacH b0117 0.32 +1.3 130,003 + yacH b0117 0.32 -0.5 130,041 + yacH b0117 0.34 -0.0 130,086 + yacH b0117 0.37 +2.3 130,109 + yacH b0117 0.38 +1.6 130,109 + yacH b0117 0.38 +1.3 130,136 - yacH b0117 0.39 +0.7 130,146 - yacH b0117 0.40 +1.1 130,151 - yacH b0117 0.40 -0.3 130,168 - yacH b0117 0.41 +2.3 130,173 - yacH b0117 0.41 -0.3 130,173 - yacH b0117 0.41 +0.3 130,180 + yacH b0117 0.42 +0.6 130,201 - yacH b0117 0.43 +0.3 130,272 - yacH b0117 0.47 -1.4 130,349 + yacH b0117 0.51 -2.3 130,376 + yacH b0117 0.52 -1.5 130,376 + yacH b0117 0.52 +1.3 130,379 + yacH b0117 0.52 +1.0 130,379 + yacH b0117 0.52 +0.3 130,382 - yacH b0117 0.53 +1.4 130,476 + yacH b0117 0.58 +0.9 130,506 + yacH b0117 0.59 +1.9 130,521 + yacH b0117 0.60 +0.9 130,521 + yacH b0117 0.60 -2.3 130,576 + yacH b0117 0.63 -0.7 130,595 + yacH b0117 0.64 -2.4 130,615 + yacH b0117 0.65 +0.3 130,615 + yacH b0117 0.65 -2.9 130,649 - yacH b0117 0.67 -1.5 130,649 - yacH b0117 0.67 +0.3 130,693 + yacH b0117 0.69 +0.8 130,732 + yacH b0117 0.71 -1.6 130,742 + yacH b0117 0.72 +1.3 130,742 + yacH b0117 0.72 +3.9 130,796 + yacH b0117 0.75 -1.4 130,871 + yacH b0117 0.79 +1.4 130,879 - yacH b0117 0.79 +0.9 130,930 + yacH b0117 0.82 +1.3 131,045 - yacH b0117 0.88 -1.0 131,228 - -1.0 131,228 - -1.7 131,369 + +1.3 131,369 + +0.3 131,389 - -2.0 131,518 + -3.2
Or see this region's nucleotide sequence