Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yabI and thiQ are separated by 113 nucleotides thiQ and thiP overlap by 17 nucleotides thiP and tbpA overlap by 25 nucleotides
b0065: yabI - conserved inner membrane protein (NCBI), at 71,351 to 72,115
yabI
b0066: thiQ - thiamin transporter subunit (NCBI), at 72,229 to 72,927
thiQ
b0067: thiP - thiamin ABC transporter membrane protein (NCBI), at 72,911 to 74,521
thiP
b0068: tbpA - thiamin transporter subunit (NCBI), at 74,497 to 75,480
tbpA
Position (kb) 72
73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 71.976 kb on - strand, within yabI at 72.058 kb on - strand at 72.058 kb on - strand at 72.077 kb on + strand at 72.081 kb on + strand at 72.081 kb on + strand at 72.116 kb on - strand at 72.117 kb on + strand at 72.117 kb on + strand at 72.118 kb on - strand at 72.159 kb on - strand at 72.181 kb on + strand at 72.203 kb on - strand at 72.212 kb on + strand at 72.212 kb on + strand at 72.239 kb on + strand at 72.239 kb on + strand at 72.247 kb on - strand at 72.249 kb on + strand at 72.249 kb on + strand at 72.249 kb on + strand at 72.257 kb on + strand at 72.272 kb on - strand at 72.321 kb on + strand, within thiQ at 72.321 kb on + strand, within thiQ at 72.354 kb on + strand, within thiQ at 72.364 kb on + strand, within thiQ at 72.370 kb on - strand, within thiQ at 72.372 kb on - strand, within thiQ at 72.372 kb on - strand, within thiQ at 72.372 kb on - strand, within thiQ at 72.372 kb on - strand, within thiQ at 72.379 kb on - strand, within thiQ at 72.415 kb on - strand, within thiQ at 72.500 kb on - strand, within thiQ at 72.500 kb on - strand, within thiQ at 72.513 kb on - strand, within thiQ at 72.513 kb on - strand, within thiQ at 72.587 kb on + strand, within thiQ at 72.593 kb on - strand, within thiQ at 72.607 kb on - strand, within thiQ at 72.630 kb on + strand, within thiQ at 72.734 kb on + strand, within thiQ at 72.734 kb on + strand, within thiQ at 72.742 kb on - strand, within thiQ at 72.856 kb on + strand, within thiQ at 72.878 kb on - strand at 72.878 kb on - strand at 73.002 kb on - strand at 73.002 kb on - strand at 73.102 kb on - strand, within thiP at 73.152 kb on - strand, within thiP at 73.212 kb on + strand, within thiP at 73.261 kb on + strand, within thiP at 73.261 kb on + strand, within thiP at 73.334 kb on + strand, within thiP at 73.345 kb on + strand, within thiP at 73.403 kb on + strand, within thiP at 73.465 kb on - strand, within thiP at 73.468 kb on - strand, within thiP at 73.468 kb on - strand, within thiP at 73.475 kb on + strand, within thiP at 73.476 kb on - strand, within thiP at 73.480 kb on + strand, within thiP at 73.488 kb on - strand, within thiP at 73.488 kb on - strand, within thiP at 73.512 kb on + strand, within thiP at 73.515 kb on - strand, within thiP at 73.515 kb on - strand, within thiP at 73.529 kb on - strand, within thiP at 73.530 kb on - strand, within thiP at 73.532 kb on - strand, within thiP at 73.533 kb on + strand, within thiP at 73.636 kb on - strand, within thiP at 73.645 kb on - strand, within thiP at 73.645 kb on - strand, within thiP at 73.645 kb on - strand, within thiP at 73.645 kb on - strand, within thiP at 73.666 kb on + strand, within thiP at 73.666 kb on + strand, within thiP at 73.689 kb on - strand, within thiP at 73.693 kb on - strand, within thiP at 73.693 kb on - strand, within thiP at 73.700 kb on - strand, within thiP at 73.700 kb on - strand, within thiP at 73.707 kb on + strand, within thiP at 73.707 kb on + strand, within thiP at 73.722 kb on + strand, within thiP at 73.724 kb on - strand, within thiP at 73.724 kb on - strand, within thiP at 73.742 kb on + strand, within thiP at 73.750 kb on - strand, within thiP at 73.778 kb on - strand, within thiP at 73.778 kb on - strand, within thiP at 73.820 kb on - strand, within thiP at 73.848 kb on - strand, within thiP at 73.849 kb on - strand, within thiP at 73.849 kb on - strand, within thiP at 73.951 kb on - strand, within thiP at 73.996 kb on + strand, within thiP at 73.996 kb on + strand, within thiP at 74.015 kb on - strand, within thiP at 74.015 kb on - strand, within thiP at 74.047 kb on + strand, within thiP at 74.048 kb on - strand, within thiP at 74.048 kb on - strand, within thiP at 74.109 kb on + strand, within thiP at 74.109 kb on + strand, within thiP at 74.116 kb on + strand, within thiP at 74.116 kb on + strand, within thiP at 74.128 kb on + strand, within thiP at 74.128 kb on + strand, within thiP at 74.128 kb on + strand, within thiP at 74.128 kb on + strand, within thiP at 74.232 kb on - strand, within thiP at 74.315 kb on + strand, within thiP at 74.360 kb on + strand, within thiP at 74.383 kb on - strand at 74.404 kb on - strand at 74.420 kb on - strand at 74.420 kb on - strand at 74.453 kb on + strand at 74.453 kb on - strand at 74.502 kb on + strand at 74.541 kb on - strand at 74.541 kb on - strand at 74.569 kb on - strand at 74.569 kb on - strand at 74.659 kb on - strand, within tbpA at 74.659 kb on - strand, within tbpA at 74.668 kb on - strand, within tbpA at 74.689 kb on - strand, within tbpA at 74.757 kb on - strand, within tbpA at 74.862 kb on - strand, within tbpA at 74.875 kb on + strand, within tbpA at 74.890 kb on - strand, within tbpA at 74.895 kb on - strand, within tbpA at 74.895 kb on - strand, within tbpA at 74.895 kb on - strand, within tbpA at 74.945 kb on - strand, within tbpA at 74.969 kb on + strand, within tbpA at 75.002 kb on - strand, within tbpA at 75.124 kb on + strand, within tbpA at 75.124 kb on + strand, within tbpA at 75.167 kb on - strand, within tbpA at 75.167 kb on - strand, within tbpA at 75.170 kb on + strand, within tbpA at 75.170 kb on + strand, within tbpA at 75.214 kb on - strand, within tbpA at 75.227 kb on + strand, within tbpA at 75.238 kb on + strand, within tbpA at 75.238 kb on + strand, within tbpA at 75.249 kb on - strand, within tbpA at 75.307 kb on + strand, within tbpA at 75.364 kb on - strand, within tbpA at 75.403 kb on + strand at 75.411 kb on - strand at 75.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 71,976 - yabI b0065 0.82 +0.7 72,058 - +1.3 72,058 - -0.5 72,077 + +0.3 72,081 + +1.0 72,081 + +0.5 72,116 - +0.7 72,117 + -1.5 72,117 + +0.3 72,118 - +0.8 72,159 - -1.0 72,181 + +4.1 72,203 - -0.0 72,212 + -1.0 72,212 + +0.3 72,239 + +0.3 72,239 + +0.3 72,247 - -0.5 72,249 + -0.9 72,249 + -0.4 72,249 + -1.5 72,257 + +0.3 72,272 - -0.0 72,321 + thiQ b0066 0.13 -0.3 72,321 + thiQ b0066 0.13 -0.9 72,354 + thiQ b0066 0.18 -0.4 72,364 + thiQ b0066 0.19 -1.9 72,370 - thiQ b0066 0.20 -1.1 72,372 - thiQ b0066 0.20 +0.7 72,372 - thiQ b0066 0.20 +0.3 72,372 - thiQ b0066 0.20 -0.0 72,372 - thiQ b0066 0.20 +1.0 72,379 - thiQ b0066 0.21 -1.0 72,415 - thiQ b0066 0.27 +0.3 72,500 - thiQ b0066 0.39 -1.0 72,500 - thiQ b0066 0.39 -0.7 72,513 - thiQ b0066 0.41 -0.3 72,513 - thiQ b0066 0.41 -1.3 72,587 + thiQ b0066 0.51 +2.5 72,593 - thiQ b0066 0.52 +0.4 72,607 - thiQ b0066 0.54 -2.0 72,630 + thiQ b0066 0.57 +0.9 72,734 + thiQ b0066 0.72 -1.5 72,734 + thiQ b0066 0.72 -0.7 72,742 - thiQ b0066 0.73 -0.3 72,856 + thiQ b0066 0.90 -1.3 72,878 - -1.5 72,878 - -1.3 73,002 - -1.1 73,002 - +1.0 73,102 - thiP b0067 0.12 -0.3 73,152 - thiP b0067 0.15 -1.9 73,212 + thiP b0067 0.19 -0.7 73,261 + thiP b0067 0.22 -0.0 73,261 + thiP b0067 0.22 -1.3 73,334 + thiP b0067 0.26 +1.5 73,345 + thiP b0067 0.27 +1.9 73,403 + thiP b0067 0.31 -0.1 73,465 - thiP b0067 0.34 -1.5 73,468 - thiP b0067 0.35 -1.3 73,468 - thiP b0067 0.35 +0.7 73,475 + thiP b0067 0.35 +1.3 73,476 - thiP b0067 0.35 -1.0 73,480 + thiP b0067 0.35 -0.5 73,488 - thiP b0067 0.36 -1.0 73,488 - thiP b0067 0.36 -1.7 73,512 + thiP b0067 0.37 -1.3 73,515 - thiP b0067 0.37 -0.5 73,515 - thiP b0067 0.37 -2.4 73,529 - thiP b0067 0.38 -0.2 73,530 - thiP b0067 0.38 -1.5 73,532 - thiP b0067 0.39 +0.3 73,533 + thiP b0067 0.39 +1.1 73,636 - thiP b0067 0.45 -2.1 73,645 - thiP b0067 0.46 -2.4 73,645 - thiP b0067 0.46 +0.6 73,645 - thiP b0067 0.46 -1.0 73,645 - thiP b0067 0.46 -1.3 73,666 + thiP b0067 0.47 -0.9 73,666 + thiP b0067 0.47 -0.8 73,689 - thiP b0067 0.48 +0.7 73,693 - thiP b0067 0.49 -2.0 73,693 - thiP b0067 0.49 -1.3 73,700 - thiP b0067 0.49 -1.5 73,700 - thiP b0067 0.49 -0.7 73,707 + thiP b0067 0.49 +0.6 73,707 + thiP b0067 0.49 +0.5 73,722 + thiP b0067 0.50 -0.8 73,724 - thiP b0067 0.50 +2.2 73,724 - thiP b0067 0.50 -1.3 73,742 + thiP b0067 0.52 -0.4 73,750 - thiP b0067 0.52 +2.4 73,778 - thiP b0067 0.54 +0.3 73,778 - thiP b0067 0.54 +0.8 73,820 - thiP b0067 0.56 -0.3 73,848 - thiP b0067 0.58 +2.6 73,849 - thiP b0067 0.58 -1.2 73,849 - thiP b0067 0.58 -1.0 73,951 - thiP b0067 0.65 +1.6 73,996 + thiP b0067 0.67 -2.0 73,996 + thiP b0067 0.67 -1.3 74,015 - thiP b0067 0.69 -0.7 74,015 - thiP b0067 0.69 -1.7 74,047 + thiP b0067 0.71 +1.2 74,048 - thiP b0067 0.71 +1.0 74,048 - thiP b0067 0.71 -1.3 74,109 + thiP b0067 0.74 -1.5 74,109 + thiP b0067 0.74 -0.8 74,116 + thiP b0067 0.75 -1.0 74,116 + thiP b0067 0.75 +0.3 74,128 + thiP b0067 0.76 +0.5 74,128 + thiP b0067 0.76 -2.7 74,128 + thiP b0067 0.76 +1.5 74,128 + thiP b0067 0.76 -2.0 74,232 - thiP b0067 0.82 -0.5 74,315 + thiP b0067 0.87 -0.1 74,360 + thiP b0067 0.90 -0.7 74,383 - +0.3 74,404 - +0.9 74,420 - -1.5 74,420 - -0.2 74,453 + +2.6 74,453 - -1.3 74,502 + -1.3 74,541 - +1.4 74,541 - -1.1 74,569 - +0.0 74,569 - -1.0 74,659 - tbpA b0068 0.16 +1.3 74,659 - tbpA b0068 0.16 -0.4 74,668 - tbpA b0068 0.17 +2.0 74,689 - tbpA b0068 0.20 -0.7 74,757 - tbpA b0068 0.26 -0.3 74,862 - tbpA b0068 0.37 -0.1 74,875 + tbpA b0068 0.38 +1.2 74,890 - tbpA b0068 0.40 +1.5 74,895 - tbpA b0068 0.40 +0.3 74,895 - tbpA b0068 0.40 -0.3 74,895 - tbpA b0068 0.40 +1.3 74,945 - tbpA b0068 0.46 +0.2 74,969 + tbpA b0068 0.48 -0.7 75,002 - tbpA b0068 0.51 +0.5 75,124 + tbpA b0068 0.64 -0.9 75,124 + tbpA b0068 0.64 -1.2 75,167 - tbpA b0068 0.68 -1.3 75,167 - tbpA b0068 0.68 +0.7 75,170 + tbpA b0068 0.68 +0.6 75,170 + tbpA b0068 0.68 -2.0 75,214 - tbpA b0068 0.73 -1.5 75,227 + tbpA b0068 0.74 -0.7 75,238 + tbpA b0068 0.75 +1.2 75,238 + tbpA b0068 0.75 -0.3 75,249 - tbpA b0068 0.76 -0.9 75,307 + tbpA b0068 0.82 +2.5 75,364 - tbpA b0068 0.88 +0.7 75,403 + -1.1 75,411 - +0.7 75,411 - -1.3
Or see this region's nucleotide sequence