Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaU and kefF are separated by 107 nucleotides kefF and kefC overlap by 8 nucleotides kefC and folA are separated by 191 nucleotides folA and apaH are separated by 77 nucleotides
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
b0046: kefF - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 47,246 to 47,776
kefF
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302
folA
b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222
apaH
Position (kb)
47
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 46.800 kb on + strand, within yaaU at 46.920 kb on - strand, within yaaU at 47.003 kb on + strand, within yaaU at 47.067 kb on + strand at 47.097 kb on - strand at 47.097 kb on - strand at 47.113 kb on - strand at 47.113 kb on - strand at 47.202 kb on - strand at 47.225 kb on + strand at 47.225 kb on + strand at 47.247 kb on - strand at 47.269 kb on + strand at 47.359 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.402 kb on - strand, within kefF at 47.412 kb on - strand, within kefF at 47.446 kb on + strand, within kefF at 47.454 kb on + strand, within kefF at 47.503 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.639 kb on + strand, within kefF at 47.651 kb on + strand, within kefF at 47.675 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.730 kb on + strand at 47.762 kb on + strand at 47.774 kb on - strand at 47.784 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.832 kb on + strand at 47.832 kb on + strand at 47.836 kb on + strand at 47.848 kb on + strand at 47.859 kb on + strand at 47.921 kb on + strand at 47.948 kb on + strand at 47.948 kb on + strand at 47.958 kb on + strand, within kefC at 47.958 kb on + strand, within kefC at 47.977 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.031 kb on + strand, within kefC at 48.227 kb on + strand, within kefC at 48.227 kb on - strand, within kefC at 48.228 kb on + strand, within kefC at 48.235 kb on - strand, within kefC at 48.253 kb on - strand, within kefC at 48.348 kb on + strand, within kefC at 48.394 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.614 kb on + strand, within kefC at 48.760 kb on + strand, within kefC at 48.760 kb on + strand, within kefC at 48.786 kb on - strand, within kefC at 48.907 kb on + strand, within kefC at 48.958 kb on + strand, within kefC at 48.968 kb on - strand, within kefC at 49.068 kb on - strand, within kefC at 49.068 kb on - strand, within kefC at 49.149 kb on - strand, within kefC at 49.149 kb on - strand, within kefC at 49.202 kb on + strand, within kefC at 49.281 kb on + strand, within kefC at 49.289 kb on - strand, within kefC at 49.289 kb on - strand, within kefC at 49.334 kb on + strand, within kefC at 49.334 kb on + strand, within kefC at 49.356 kb on - strand, within kefC at 49.356 kb on - strand, within kefC at 49.369 kb on + strand, within kefC at 49.369 kb on + strand, within kefC at 49.409 kb on - strand, within kefC at 49.431 kb on + strand, within kefC at 49.487 kb on - strand at 49.493 kb on - strand at 49.709 kb on + strand at 50.361 kb on + strand at 50.361 kb on + strand at 50.370 kb on - strand at 50.376 kb on + strand at 50.461 kb on + strand at 50.469 kb on - strand, within apaH at 50.501 kb on + strand, within apaH at 50.507 kb on - strand, within apaH at 50.568 kb on - strand, within apaH
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 46,800 + yaaU b0045 0.75 +0.3 46,920 - yaaU b0045 0.84 -0.9 47,003 + yaaU b0045 0.90 -0.3 47,067 + -0.1 47,097 - -1.9 47,097 - -0.2 47,113 - +0.5 47,113 - +0.3 47,202 - -0.3 47,225 + -1.0 47,225 + -1.0 47,247 - -1.1 47,269 + -3.5 47,359 - kefF b0046 0.21 +1.6 47,364 - kefF b0046 0.22 +1.8 47,364 - kefF b0046 0.22 +1.5 47,364 - kefF b0046 0.22 +1.8 47,364 - kefF b0046 0.22 -0.5 47,402 - kefF b0046 0.29 +1.5 47,412 - kefF b0046 0.31 -0.5 47,446 + kefF b0046 0.38 +1.7 47,454 + kefF b0046 0.39 +2.9 47,503 + kefF b0046 0.48 +4.9 47,504 + kefF b0046 0.49 -1.9 47,504 + kefF b0046 0.49 +0.2 47,639 + kefF b0046 0.74 +0.4 47,651 + kefF b0046 0.76 -1.6 47,675 + kefF b0046 0.81 -0.0 47,681 + kefF b0046 0.82 -0.3 47,681 + kefF b0046 0.82 -1.5 47,730 + +1.9 47,762 + -0.8 47,774 - +2.7 47,784 - -0.7 47,823 + +0.3 47,823 + -2.0 47,832 + +3.5 47,832 + +1.3 47,836 + -0.3 47,848 + +0.9 47,859 + -0.7 47,921 + +0.2 47,948 + -1.0 47,948 + +3.5 47,958 + kefC b0047 0.10 +1.3 47,958 + kefC b0047 0.10 +0.7 47,977 - kefC b0047 0.11 +2.1 48,009 - kefC b0047 0.13 +1.8 48,009 - kefC b0047 0.13 +2.4 48,009 - kefC b0047 0.13 +1.3 48,031 + kefC b0047 0.14 +1.2 48,227 + kefC b0047 0.25 -0.9 48,227 - kefC b0047 0.25 +2.3 48,228 + kefC b0047 0.25 -1.4 48,235 - kefC b0047 0.25 +0.5 48,253 - kefC b0047 0.26 -0.0 48,348 + kefC b0047 0.31 +0.4 48,394 + kefC b0047 0.34 +0.3 48,408 + kefC b0047 0.34 +2.5 48,408 + kefC b0047 0.34 +0.8 48,569 + kefC b0047 0.43 -1.0 48,569 + kefC b0047 0.43 +0.9 48,614 + kefC b0047 0.45 -1.9 48,760 + kefC b0047 0.53 +0.6 48,760 + kefC b0047 0.53 -0.0 48,786 - kefC b0047 0.55 +0.7 48,907 + kefC b0047 0.61 -1.0 48,958 + kefC b0047 0.64 +1.2 48,968 - kefC b0047 0.64 -1.5 49,068 - kefC b0047 0.70 -0.1 49,068 - kefC b0047 0.70 -1.8 49,149 - kefC b0047 0.74 +1.9 49,149 - kefC b0047 0.74 +2.1 49,202 + kefC b0047 0.77 +1.0 49,281 + kefC b0047 0.81 +1.4 49,289 - kefC b0047 0.82 +1.9 49,289 - kefC b0047 0.82 +0.6 49,334 + kefC b0047 0.84 -1.0 49,334 + kefC b0047 0.84 -1.4 49,356 - kefC b0047 0.85 -0.9 49,356 - kefC b0047 0.85 +0.5 49,369 + kefC b0047 0.86 -0.6 49,369 + kefC b0047 0.86 +0.6 49,409 - kefC b0047 0.88 -0.6 49,431 + kefC b0047 0.89 +0.2 49,487 - +1.9 49,493 - +2.0 49,709 + -1.8 50,361 + +1.3 50,361 + -0.3 50,370 - -0.3 50,376 + +2.2 50,461 + -2.5 50,469 - apaH b0049 0.11 -0.6 50,501 + apaH b0049 0.14 +3.1 50,507 - apaH b0049 0.15 +0.7 50,568 - apaH b0049 0.22 +1.6
Or see this region's nucleotide sequence