Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaK and dnaJ are separated by 88 nucleotides dnaJ and insL-1 are separated by 146 nucleotides
b0014: dnaK - molecular chaperone DnaK (NCBI), at 12,163 to 14,079
dnaK
b0015: dnaJ - chaperone Hsp40, co-chaperone with DnaK (NCBI), at 14,168 to 15,298
dnaJ
b0016: insL-1 - IS186/IS421 transposase (NCBI), at 15,445 to 16,557
insL-1
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.673 kb on + strand, within dnaK at 13.859 kb on + strand, within dnaK at 13.894 kb on + strand at 13.894 kb on + strand at 14.116 kb on + strand at 14.145 kb on + strand at 14.245 kb on - strand at 14.260 kb on - strand at 14.264 kb on + strand at 14.264 kb on + strand at 14.325 kb on + strand, within dnaJ at 14.371 kb on + strand, within dnaJ at 14.374 kb on - strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.508 kb on + strand, within dnaJ at 14.521 kb on + strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.594 kb on - strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on + strand, within dnaJ at 14.596 kb on - strand, within dnaJ at 14.627 kb on - strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.664 kb on + strand, within dnaJ at 14.672 kb on - strand, within dnaJ at 14.682 kb on - strand, within dnaJ at 14.702 kb on + strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.713 kb on - strand, within dnaJ at 14.745 kb on + strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.781 kb on - strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.829 kb on + strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.842 kb on - strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.859 kb on + strand, within dnaJ at 14.923 kb on + strand, within dnaJ at 14.959 kb on - strand, within dnaJ at 15.087 kb on + strand, within dnaJ at 15.087 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.111 kb on + strand, within dnaJ at 15.143 kb on + strand, within dnaJ at 15.164 kb on - strand, within dnaJ at 15.235 kb on - strand at 15.235 kb on - strand at 15.289 kb on + strand at 15.389 kb on - strand at 15.410 kb on - strand at 15.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 13,673 + dnaK b0014 0.79 -0.7 13,673 + dnaK b0014 0.79 +1.3 13,673 + dnaK b0014 0.79 -0.7 13,859 + dnaK b0014 0.88 -1.3 13,894 + -2.9 13,894 + +1.3 14,116 + -1.7 14,145 + -1.5 14,245 - -1.4 14,260 - -2.0 14,264 + -2.1 14,264 + -2.0 14,325 + dnaJ b0015 0.14 -2.1 14,371 + dnaJ b0015 0.18 -0.3 14,374 - dnaJ b0015 0.18 -1.7 14,508 + dnaJ b0015 0.30 -0.8 14,508 + dnaJ b0015 0.30 -1.5 14,521 + dnaJ b0015 0.31 -0.8 14,594 - dnaJ b0015 0.38 -1.3 14,594 - dnaJ b0015 0.38 -2.1 14,594 - dnaJ b0015 0.38 +0.1 14,596 + dnaJ b0015 0.38 -0.7 14,596 + dnaJ b0015 0.38 +0.3 14,596 - dnaJ b0015 0.38 -1.9 14,627 - dnaJ b0015 0.41 -0.8 14,664 + dnaJ b0015 0.44 -1.3 14,664 + dnaJ b0015 0.44 -1.7 14,672 - dnaJ b0015 0.45 -0.7 14,682 - dnaJ b0015 0.45 -0.0 14,702 + dnaJ b0015 0.47 +0.1 14,713 - dnaJ b0015 0.48 -1.6 14,713 - dnaJ b0015 0.48 -1.3 14,713 - dnaJ b0015 0.48 -1.0 14,745 + dnaJ b0015 0.51 -1.7 14,781 - dnaJ b0015 0.54 -1.6 14,781 - dnaJ b0015 0.54 -0.5 14,829 + dnaJ b0015 0.58 -1.5 14,829 + dnaJ b0015 0.58 -0.3 14,842 - dnaJ b0015 0.60 -0.3 14,842 - dnaJ b0015 0.60 -0.7 14,859 + dnaJ b0015 0.61 -0.5 14,859 + dnaJ b0015 0.61 -0.0 14,923 + dnaJ b0015 0.67 -1.7 14,959 - dnaJ b0015 0.70 -1.4 15,087 + dnaJ b0015 0.81 -1.0 15,087 + dnaJ b0015 0.81 -1.7 15,111 + dnaJ b0015 0.83 -1.3 15,111 + dnaJ b0015 0.83 -2.1 15,111 + dnaJ b0015 0.83 -1.9 15,143 + dnaJ b0015 0.86 +0.2 15,164 - dnaJ b0015 0.88 -1.0 15,235 - -0.7 15,235 - +0.6 15,289 + +2.1 15,389 - -1.0 15,410 - +0.6 15,439 - -0.7
Or see this region's nucleotide sequence