Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF980 and GFF981 are separated by 296 nucleotides GFF981 and GFF982 are separated by 352 nucleotides GFF982 and GFF983 are separated by 238 nucleotides GFF983 and GFF984 are separated by 5 nucleotides
GFF980 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 165,178 to 166,191
GFF980
GFF981 - FIG01045355: hypothetical protein, at 166,488 to 166,640
GFF981
GFF982 - Putative cytoplasmic protein USSDB7A, at 166,993 to 167,439
GFF982
GFF983 - Aspartate racemase (EC 5.1.1.13), at 167,678 to 168,412
GFF983
GFF984 - LysR family transcriptional regulator QseA, at 168,418 to 169,326
GFF984
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 166.032 kb on + strand, within GFF980 at 166.076 kb on + strand, within GFF980 at 166.077 kb on - strand, within GFF980 at 166.077 kb on - strand, within GFF980 at 166.205 kb on - strand at 166.205 kb on - strand at 166.212 kb on - strand at 166.359 kb on + strand at 166.435 kb on + strand at 166.787 kb on + strand at 166.843 kb on - strand at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.127 kb on + strand, within GFF982 at 167.128 kb on - strand, within GFF982 at 167.166 kb on + strand, within GFF982 at 167.179 kb on + strand, within GFF982 at 167.228 kb on + strand, within GFF982 at 167.228 kb on + strand, within GFF982 at 167.263 kb on - strand, within GFF982 at 167.265 kb on + strand, within GFF982 at 167.271 kb on + strand, within GFF982 at 167.355 kb on + strand, within GFF982 at 167.362 kb on - strand, within GFF982 at 167.479 kb on + strand at 167.528 kb on + strand at 167.529 kb on - strand at 167.824 kb on + strand, within GFF983 at 167.885 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.940 kb on - strand, within GFF983 at 167.940 kb on - strand, within GFF983 at 168.075 kb on - strand, within GFF983 at 168.075 kb on - strand, within GFF983 at 168.108 kb on + strand, within GFF983 at 168.155 kb on + strand, within GFF983 at 168.244 kb on + strand, within GFF983 at 168.245 kb on - strand, within GFF983 at 168.271 kb on + strand, within GFF983
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 166,032 + GFF980 0.84 +0.1 166,076 + GFF980 0.89 +0.1 166,077 - GFF980 0.89 -0.2 166,077 - GFF980 0.89 +0.1 166,205 - -0.8 166,205 - +1.1 166,212 - -0.2 166,359 + -0.1 166,435 + +0.3 166,787 + +0.5 166,843 - +0.5 167,125 + GFF982 0.30 -0.5 167,125 + GFF982 0.30 +0.6 167,125 + GFF982 0.30 -0.5 167,126 - GFF982 0.30 -0.7 167,126 - GFF982 0.30 -0.6 167,126 - GFF982 0.30 -0.3 167,127 + GFF982 0.30 -0.1 167,128 - GFF982 0.30 -1.1 167,166 + GFF982 0.39 -0.6 167,179 + GFF982 0.42 +0.2 167,228 + GFF982 0.53 -0.6 167,228 + GFF982 0.53 -0.2 167,263 - GFF982 0.60 -1.3 167,265 + GFF982 0.61 -1.1 167,271 + GFF982 0.62 -0.8 167,355 + GFF982 0.81 -0.7 167,362 - GFF982 0.83 -0.6 167,479 + -0.1 167,528 + -1.2 167,529 - -0.0 167,824 + GFF983 0.20 -0.7 167,885 - GFF983 0.28 -0.1 167,912 - GFF983 0.32 -0.0 167,912 - GFF983 0.32 +0.0 167,912 - GFF983 0.32 -0.4 167,940 - GFF983 0.36 +0.8 167,940 - GFF983 0.36 -0.0 168,075 - GFF983 0.54 -1.0 168,075 - GFF983 0.54 -0.4 168,108 + GFF983 0.59 -0.7 168,155 + GFF983 0.65 +0.1 168,244 + GFF983 0.77 -0.1 168,245 - GFF983 0.77 -0.1 168,271 + GFF983 0.81 -0.2
Or see this region's nucleotide sequence