Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4342 and GFF4343 are separated by 14 nucleotides GFF4343 and GFF4344 overlap by 1 nucleotides GFF4344 and GFF4345 overlap by 11 nucleotides GFF4345 and GFF4346 are separated by 10 nucleotides
GFF4342 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 123,566 to 124,453
GFF4342
GFF4343 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 124,468 to 124,797
GFF4343
GFF4344 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 124,797 to 125,411
GFF4344
GFF4345 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 125,401 to 127,392
GFF4345
GFF4346 - Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-), at 127,403 to 128,332
GFF4346
Position (kb)
125
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 124.488 kb on - strand at 124.488 kb on - strand at 124.503 kb on + strand, within GFF4343 at 124.504 kb on - strand, within GFF4343 at 124.650 kb on + strand, within GFF4343 at 124.732 kb on - strand, within GFF4343 at 124.785 kb on + strand at 124.799 kb on - strand at 124.830 kb on + strand at 125.075 kb on + strand, within GFF4344 at 125.118 kb on - strand, within GFF4344 at 125.144 kb on + strand, within GFF4344 at 125.272 kb on + strand, within GFF4344 at 125.273 kb on - strand, within GFF4344 at 125.277 kb on - strand, within GFF4344 at 125.431 kb on - strand at 125.484 kb on - strand at 125.484 kb on - strand at 125.487 kb on - strand at 125.744 kb on + strand, within GFF4345 at 125.978 kb on + strand, within GFF4345 at 125.978 kb on + strand, within GFF4345 at 125.979 kb on - strand, within GFF4345 at 125.989 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.081 kb on - strand, within GFF4345 at 126.088 kb on - strand, within GFF4345 at 126.281 kb on + strand, within GFF4345 at 126.569 kb on + strand, within GFF4345 at 126.569 kb on + strand, within GFF4345 at 126.569 kb on + strand, within GFF4345 at 126.570 kb on - strand, within GFF4345 at 126.620 kb on + strand, within GFF4345 at 126.792 kb on + strand, within GFF4345 at 126.843 kb on + strand, within GFF4345 at 126.851 kb on + strand, within GFF4345 at 126.851 kb on + strand, within GFF4345 at 126.852 kb on - strand, within GFF4345 at 126.852 kb on - strand, within GFF4345 at 127.210 kb on - strand at 127.211 kb on + strand at 127.211 kb on + strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.231 kb on - strand at 127.266 kb on - strand at 127.276 kb on - strand at 127.348 kb on - strand at 127.394 kb on + strand at 127.395 kb on + strand at 127.395 kb on + strand at 127.396 kb on - strand at 127.396 kb on - strand at 127.444 kb on + strand at 127.445 kb on - strand at 127.445 kb on - strand at 127.529 kb on - strand, within GFF4346 at 127.529 kb on - strand, within GFF4346 at 127.566 kb on + strand, within GFF4346 at 127.567 kb on - strand, within GFF4346 at 127.595 kb on - strand, within GFF4346 at 127.618 kb on + strand, within GFF4346 at 127.693 kb on + strand, within GFF4346 at 127.752 kb on + strand, within GFF4346 at 127.752 kb on + strand, within GFF4346 at 127.810 kb on - strand, within GFF4346 at 127.936 kb on - strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.143 kb on + strand, within GFF4346 at 128.316 kb on - strand at 128.338 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 124,488 - -0.2 124,488 - -1.8 124,503 + GFF4343 0.11 -0.1 124,504 - GFF4343 0.11 -1.1 124,650 + GFF4343 0.55 -0.6 124,732 - GFF4343 0.80 -0.4 124,785 + -0.9 124,799 - -0.4 124,830 + -1.2 125,075 + GFF4344 0.45 -0.7 125,118 - GFF4344 0.52 -0.1 125,144 + GFF4344 0.56 +0.0 125,272 + GFF4344 0.77 -0.6 125,273 - GFF4344 0.77 -0.9 125,277 - GFF4344 0.78 -0.7 125,431 - -1.6 125,484 - -0.3 125,484 - -1.5 125,487 - -0.5 125,744 + GFF4345 0.17 -0.9 125,978 + GFF4345 0.29 +0.1 125,978 + GFF4345 0.29 -0.7 125,979 - GFF4345 0.29 -1.8 125,989 - GFF4345 0.30 -0.9 126,081 - GFF4345 0.34 -0.8 126,081 - GFF4345 0.34 -0.5 126,081 - GFF4345 0.34 -0.2 126,081 - GFF4345 0.34 -0.0 126,088 - GFF4345 0.34 -0.2 126,281 + GFF4345 0.44 -0.5 126,569 + GFF4345 0.59 -0.5 126,569 + GFF4345 0.59 -1.2 126,569 + GFF4345 0.59 -0.6 126,570 - GFF4345 0.59 -0.4 126,620 + GFF4345 0.61 -0.1 126,792 + GFF4345 0.70 -0.5 126,843 + GFF4345 0.72 +0.0 126,851 + GFF4345 0.73 -0.2 126,851 + GFF4345 0.73 -1.8 126,852 - GFF4345 0.73 +0.2 126,852 - GFF4345 0.73 +0.1 127,210 - -1.2 127,211 + -0.0 127,211 + -0.4 127,212 - +0.6 127,212 - -1.0 127,212 - +1.1 127,212 - -0.3 127,212 - -1.1 127,231 - -0.1 127,266 - -0.6 127,276 - -0.7 127,348 - -1.2 127,394 + -0.8 127,395 + -0.2 127,395 + -1.1 127,396 - -0.2 127,396 - -0.4 127,444 + -1.8 127,445 - -0.2 127,445 - -0.5 127,529 - GFF4346 0.14 +0.2 127,529 - GFF4346 0.14 +0.8 127,566 + GFF4346 0.18 -0.6 127,567 - GFF4346 0.18 -0.8 127,595 - GFF4346 0.21 -0.4 127,618 + GFF4346 0.23 -1.0 127,693 + GFF4346 0.31 -0.1 127,752 + GFF4346 0.38 -0.2 127,752 + GFF4346 0.38 -0.8 127,810 - GFF4346 0.44 -1.6 127,936 - GFF4346 0.57 -0.4 128,127 + GFF4346 0.78 -1.2 128,127 + GFF4346 0.78 -0.5 128,143 + GFF4346 0.80 -2.6 128,316 - -1.1 128,338 + -0.6
Or see this region's nucleotide sequence