Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4253 and GFF4254 are separated by 494 nucleotides GFF4254 and GFF4255 overlap by 4 nucleotides
GFF4253 - Type III restriction-modification system StyLTI enzyme res (EC 3.1.21.5), at 35,514 to 38,483
GFF4253
GFF4254 - putative Cytochrome bd2, subunit I, at 38,978 to 40,381
GFF4254
GFF4255 - putative Cytochrome bd2, subunit II, at 40,378 to 41,388
GFF4255
Position (kb) 38
39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 37.980 kb on + strand, within GFF4253 at 38.051 kb on + strand, within GFF4253 at 38.120 kb on - strand, within GFF4253 at 38.237 kb on + strand at 38.370 kb on - strand at 38.575 kb on - strand at 38.619 kb on + strand at 38.619 kb on + strand at 38.619 kb on + strand at 38.742 kb on - strand at 38.768 kb on + strand at 38.768 kb on + strand at 38.772 kb on + strand at 38.776 kb on - strand at 38.872 kb on + strand at 38.872 kb on + strand at 38.959 kb on + strand at 38.961 kb on + strand at 38.961 kb on + strand at 39.097 kb on - strand at 39.250 kb on + strand, within GFF4254 at 39.367 kb on + strand, within GFF4254 at 39.374 kb on - strand, within GFF4254 at 39.471 kb on + strand, within GFF4254 at 39.532 kb on + strand, within GFF4254 at 39.532 kb on + strand, within GFF4254 at 39.533 kb on - strand, within GFF4254 at 39.552 kb on + strand, within GFF4254 at 39.604 kb on + strand, within GFF4254 at 39.784 kb on + strand, within GFF4254 at 39.871 kb on + strand, within GFF4254 at 40.119 kb on + strand, within GFF4254 at 40.184 kb on - strand, within GFF4254 at 40.226 kb on + strand, within GFF4254 at 40.252 kb on + strand at 40.253 kb on - strand at 40.253 kb on - strand at 40.256 kb on + strand at 40.257 kb on - strand at 40.257 kb on - strand at 40.411 kb on - strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.435 kb on + strand at 40.436 kb on - strand at 40.436 kb on - strand at 40.436 kb on - strand at 40.603 kb on + strand, within GFF4255 at 40.604 kb on - strand, within GFF4255 at 40.660 kb on - strand, within GFF4255 at 40.660 kb on - strand, within GFF4255 at 40.888 kb on + strand, within GFF4255 at 40.888 kb on + strand, within GFF4255 at 41.067 kb on - strand, within GFF4255 at 41.071 kb on + strand, within GFF4255 at 41.072 kb on - strand, within GFF4255 at 41.341 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 37,980 + GFF4253 0.83 -0.2 38,051 + GFF4253 0.85 -0.0 38,120 - GFF4253 0.88 -0.3 38,237 + -0.1 38,370 - +0.3 38,575 - -1.0 38,619 + -0.7 38,619 + -1.0 38,619 + -0.0 38,742 - +0.4 38,768 + +0.3 38,768 + +0.2 38,772 + -0.2 38,776 - -0.3 38,872 + +0.0 38,872 + -0.2 38,959 + +0.3 38,961 + +0.4 38,961 + +0.5 39,097 - -0.5 39,250 + GFF4254 0.19 -0.1 39,367 + GFF4254 0.28 +0.3 39,374 - GFF4254 0.28 -0.5 39,471 + GFF4254 0.35 +0.1 39,532 + GFF4254 0.39 -0.1 39,532 + GFF4254 0.39 -1.1 39,533 - GFF4254 0.40 +0.0 39,552 + GFF4254 0.41 -0.0 39,604 + GFF4254 0.45 -0.6 39,784 + GFF4254 0.57 +0.3 39,871 + GFF4254 0.64 +1.4 40,119 + GFF4254 0.81 +1.0 40,184 - GFF4254 0.86 +0.4 40,226 + GFF4254 0.89 -0.4 40,252 + +0.3 40,253 - +0.2 40,253 - +0.3 40,256 + -0.2 40,257 - +0.8 40,257 - +0.4 40,411 - +0.8 40,435 + +0.2 40,435 + +0.3 40,435 + +0.8 40,435 + -1.1 40,436 - +0.3 40,436 - +0.2 40,436 - -0.0 40,603 + GFF4255 0.22 -1.0 40,604 - GFF4255 0.22 +0.3 40,660 - GFF4255 0.28 +0.1 40,660 - GFF4255 0.28 -0.6 40,888 + GFF4255 0.50 +0.0 40,888 + GFF4255 0.50 -0.2 41,067 - GFF4255 0.68 -0.2 41,071 + GFF4255 0.69 +0.2 41,072 - GFF4255 0.69 -2.9 41,341 + +0.8
Or see this region's nucleotide sequence