Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4056 and GFF4057 are separated by 185 nucleotides GFF4057 and GFF4058 are separated by 253 nucleotides GFF4058 and GFF4059 are separated by 19 nucleotides GFF4059 and GFF4060 are separated by 129 nucleotides GFF4060 and GFF4061 are separated by 35 nucleotides
GFF4056 - Uncharacterized protein ImpC, at 19,909 to 21,417
GFF4056
GFF4057 - putative cytoplasmic protein, at 21,603 to 22,049
GFF4057
GFF4058 - Putative cytoplasmic protein USSDB7A, at 22,303 to 22,788
GFF4058
GFF4059 - Type VI secretion lipoprotein/VasD, at 22,808 to 22,963
GFF4059
GFF4060 - putative cytoplasmic protein, at 23,093 to 23,578
GFF4060
GFF4061 - putative periplasmic protein, at 23,614 to 23,946
GFF4061
Position (kb) 22
23 Strain fitness (log2 ratio) -1
0
1 at 21.419 kb on - strand at 21.421 kb on - strand at 21.423 kb on - strand at 21.527 kb on - strand at 21.599 kb on + strand at 21.765 kb on + strand, within GFF4057 at 21.765 kb on + strand, within GFF4057 at 21.767 kb on + strand, within GFF4057 at 21.767 kb on + strand, within GFF4057 at 21.768 kb on - strand, within GFF4057 at 21.768 kb on - strand, within GFF4057 at 21.779 kb on - strand, within GFF4057 at 22.043 kb on + strand at 22.483 kb on + strand, within GFF4058 at 22.483 kb on + strand, within GFF4058 at 22.950 kb on + strand at 22.997 kb on + strand at 23.044 kb on - strand at 23.065 kb on + strand at 23.318 kb on + strand, within GFF4060 at 23.663 kb on - strand, within GFF4061
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 21,419 - -0.7 21,421 - -0.4 21,423 - -0.3 21,527 - -1.2 21,599 + +0.2 21,765 + GFF4057 0.36 +0.1 21,765 + GFF4057 0.36 +0.1 21,767 + GFF4057 0.37 -1.0 21,767 + GFF4057 0.37 +0.0 21,768 - GFF4057 0.37 +0.1 21,768 - GFF4057 0.37 -0.1 21,779 - GFF4057 0.39 +0.4 22,043 + +0.1 22,483 + GFF4058 0.37 -0.9 22,483 + GFF4058 0.37 -0.5 22,950 + -0.1 22,997 + +0.4 23,044 - +0.4 23,065 + +0.1 23,318 + GFF4060 0.46 +0.7 23,663 - GFF4061 0.15 -0.5
Or see this region's nucleotide sequence