Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF379 and GFF380 overlap by 4 nucleotides GFF380 and GFF381 overlap by 8 nucleotides GFF381 and GFF382 are separated by 55 nucleotides
GFF379 - Lipopolysaccharide heptosyltransferase III (EC 2.4.1.-), at 94,203 to 95,219
GFF379
GFF380 - UDP-glucose:(heptosyl) LPS alpha1,3-glucosyltransferase WaaG (EC 2.4.1.-), at 95,216 to 96,340
GFF380
GFF381 - Lipopolysaccharide core biosynthesis protein WaaP (EC 2.7.-.-), heptosyl-I-kinase, at 96,333 to 97,130
GFF381
GFF382 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,3-glucosyltransferase WaaO (EC 2.4.1.-), at 97,186 to 97,395
GFF382
Position (kb) 95
96
97 Strain fitness (log2 ratio) -2
-1
0
1 at 94.403 kb on + strand, within GFF379 at 94.543 kb on + strand, within GFF379 at 94.547 kb on - strand, within GFF379 at 94.551 kb on + strand, within GFF379 at 94.614 kb on + strand, within GFF379 at 94.614 kb on + strand, within GFF379 at 94.614 kb on + strand, within GFF379 at 94.615 kb on - strand, within GFF379 at 94.615 kb on - strand, within GFF379 at 94.701 kb on + strand, within GFF379 at 94.701 kb on + strand, within GFF379 at 94.737 kb on - strand, within GFF379 at 94.749 kb on - strand, within GFF379 at 94.769 kb on + strand, within GFF379 at 94.850 kb on + strand, within GFF379 at 94.851 kb on - strand, within GFF379 at 94.973 kb on + strand, within GFF379 at 95.030 kb on - strand, within GFF379 at 95.198 kb on - strand at 95.239 kb on + strand at 95.240 kb on - strand at 95.325 kb on - strand at 95.417 kb on + strand, within GFF380 at 95.576 kb on + strand, within GFF380 at 95.634 kb on + strand, within GFF380 at 95.635 kb on - strand, within GFF380 at 95.650 kb on + strand, within GFF380 at 95.651 kb on - strand, within GFF380 at 95.909 kb on + strand, within GFF380 at 95.910 kb on - strand, within GFF380 at 96.015 kb on + strand, within GFF380 at 96.036 kb on + strand, within GFF380 at 96.050 kb on + strand, within GFF380 at 96.137 kb on + strand, within GFF380 at 96.138 kb on - strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.139 kb on + strand, within GFF380 at 96.266 kb on + strand at 96.285 kb on + strand at 96.319 kb on + strand at 96.319 kb on + strand at 96.320 kb on - strand at 96.330 kb on + strand at 96.364 kb on + strand at 96.488 kb on + strand, within GFF381 at 96.488 kb on + strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.536 kb on + strand, within GFF381 at 96.537 kb on - strand, within GFF381 at 96.602 kb on + strand, within GFF381 at 96.662 kb on + strand, within GFF381 at 96.663 kb on - strand, within GFF381 at 96.705 kb on + strand, within GFF381 at 96.725 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 94,403 + GFF379 0.20 -0.1 94,543 + GFF379 0.33 -0.2 94,547 - GFF379 0.34 -0.9 94,551 + GFF379 0.34 +1.4 94,614 + GFF379 0.40 +0.6 94,614 + GFF379 0.40 -0.4 94,614 + GFF379 0.40 -0.1 94,615 - GFF379 0.41 -1.2 94,615 - GFF379 0.41 +0.5 94,701 + GFF379 0.49 -0.0 94,701 + GFF379 0.49 +0.0 94,737 - GFF379 0.53 -0.4 94,749 - GFF379 0.54 -0.8 94,769 + GFF379 0.56 -0.1 94,850 + GFF379 0.64 -1.5 94,851 - GFF379 0.64 -0.4 94,973 + GFF379 0.76 -0.1 95,030 - GFF379 0.81 -0.1 95,198 - -0.0 95,239 + -0.6 95,240 - -1.7 95,325 - -1.1 95,417 + GFF380 0.18 -1.0 95,576 + GFF380 0.32 -1.2 95,634 + GFF380 0.37 -0.3 95,635 - GFF380 0.37 -0.8 95,650 + GFF380 0.39 -0.4 95,651 - GFF380 0.39 -0.6 95,909 + GFF380 0.62 +0.2 95,910 - GFF380 0.62 -0.7 96,015 + GFF380 0.71 -1.2 96,036 + GFF380 0.73 -0.2 96,050 + GFF380 0.74 -0.2 96,137 + GFF380 0.82 +0.1 96,138 - GFF380 0.82 -0.5 96,139 + GFF380 0.82 -1.3 96,139 + GFF380 0.82 -1.9 96,139 + GFF380 0.82 -0.8 96,139 + GFF380 0.82 -0.9 96,266 + -0.1 96,285 + +0.2 96,319 + -0.1 96,319 + -1.8 96,320 - -1.4 96,330 + -0.9 96,364 + +0.9 96,488 + GFF381 0.19 -1.3 96,488 + GFF381 0.19 +0.8 96,533 - GFF381 0.25 -1.2 96,533 - GFF381 0.25 -0.7 96,536 + GFF381 0.25 -0.5 96,537 - GFF381 0.26 -1.1 96,602 + GFF381 0.34 -0.8 96,662 + GFF381 0.41 -0.6 96,663 - GFF381 0.41 -1.4 96,705 + GFF381 0.47 -0.9 96,725 + GFF381 0.49 -0.8 96,776 + GFF381 0.56 -0.4 96,776 + GFF381 0.56 -0.2
Or see this region's nucleotide sequence