Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF355 and GFF356 are separated by 5 nucleotides GFF356 and GFF357 are separated by 4 nucleotides GFF357 and GFF358 are separated by 18 nucleotides GFF358 and GFF359 are separated by 54 nucleotides
GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197
GFF355
GFF356 - 'tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)' transl_table=11, at 76,203 to 76,892
GFF356
GFF357 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 76,897 to 79,008
GFF357
GFF358 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 79,027 to 79,302
GFF358
GFF359 - Guanylate kinase (EC 2.7.4.8), at 79,357 to 79,980
GFF359
Position (kb) 76
77
78
79
80 Strain fitness (log2 ratio) -2
-1
0
1 at 76.128 kb on + strand at 76.418 kb on - strand, within GFF356 at 76.593 kb on - strand, within GFF356 at 76.899 kb on - strand at 76.970 kb on - strand at 76.987 kb on - strand at 77.002 kb on - strand at 77.005 kb on - strand at 77.062 kb on - strand at 77.202 kb on + strand, within GFF357 at 77.203 kb on - strand, within GFF357 at 79.064 kb on - strand, within GFF358 at 79.303 kb on - strand at 79.307 kb on - strand at 79.414 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 76,128 + -1.8 76,418 - GFF356 0.31 -0.4 76,593 - GFF356 0.57 -0.1 76,899 - +0.1 76,970 - +0.1 76,987 - -1.3 77,002 - +0.5 77,005 - -0.4 77,062 - +0.1 77,202 + GFF357 0.14 -1.8 77,203 - GFF357 0.14 -0.7 79,064 - GFF358 0.13 -1.8 79,303 - -1.0 79,307 - -0.9 79,414 - -0.2
Or see this region's nucleotide sequence