Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3539 and GFF3540 are separated by 41 nucleotides GFF3540 and GFF3541 are separated by 89 nucleotides GFF3541 and GFF3542 are separated by 98 nucleotides
GFF3539 - Phosphoenolpyruvate synthase (EC 2.7.9.2), at 289,823 to 292,201
GFF3539
GFF3540 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 292,243 to 293,883
GFF3540
GFF3541 - Ferredoxin-like protein FixX, at 293,973 to 294,164
GFF3541
GFF3542 - Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-), at 294,263 to 295,549
GFF3542
Position (kb) 292
293
294 Strain fitness (log2 ratio) -1
0
1 at 291.250 kb on - strand, within GFF3539 at 291.342 kb on + strand, within GFF3539 at 291.370 kb on + strand, within GFF3539 at 291.377 kb on - strand, within GFF3539 at 291.608 kb on + strand, within GFF3539 at 291.928 kb on + strand, within GFF3539 at 291.928 kb on + strand, within GFF3539 at 292.062 kb on - strand at 292.199 kb on + strand at 292.205 kb on - strand at 292.205 kb on - strand at 292.278 kb on + strand at 292.279 kb on - strand at 292.367 kb on + strand at 292.367 kb on + strand at 292.448 kb on + strand, within GFF3540 at 292.448 kb on + strand, within GFF3540 at 292.449 kb on - strand, within GFF3540 at 292.486 kb on - strand, within GFF3540 at 292.530 kb on + strand, within GFF3540 at 292.644 kb on - strand, within GFF3540 at 292.704 kb on + strand, within GFF3540 at 292.884 kb on - strand, within GFF3540 at 292.884 kb on - strand, within GFF3540 at 292.941 kb on + strand, within GFF3540 at 293.050 kb on - strand, within GFF3540 at 293.069 kb on - strand, within GFF3540 at 293.250 kb on + strand, within GFF3540 at 293.875 kb on - strand at 293.914 kb on - strand at 294.035 kb on + strand, within GFF3541 at 294.036 kb on - strand, within GFF3541 at 294.119 kb on - strand, within GFF3541 at 294.152 kb on + strand at 294.189 kb on - strand at 294.252 kb on - strand at 294.266 kb on + strand at 294.596 kb on + strand, within GFF3542 at 294.616 kb on + strand, within GFF3542 at 294.716 kb on - strand, within GFF3542 at 294.839 kb on + strand, within GFF3542
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 291,250 - GFF3539 0.60 -0.9 291,342 + GFF3539 0.64 +0.0 291,370 + GFF3539 0.65 -0.8 291,377 - GFF3539 0.65 +0.2 291,608 + GFF3539 0.75 +0.6 291,928 + GFF3539 0.88 +1.0 291,928 + GFF3539 0.88 +0.9 292,062 - +0.2 292,199 + +0.2 292,205 - +0.6 292,205 - +0.2 292,278 + +0.1 292,279 - +0.3 292,367 + +0.1 292,367 + -1.3 292,448 + GFF3540 0.12 -0.1 292,448 + GFF3540 0.12 +0.2 292,449 - GFF3540 0.13 -0.2 292,486 - GFF3540 0.15 -0.9 292,530 + GFF3540 0.17 -0.1 292,644 - GFF3540 0.24 -0.1 292,704 + GFF3540 0.28 -0.3 292,884 - GFF3540 0.39 +0.4 292,884 - GFF3540 0.39 +0.2 292,941 + GFF3540 0.43 -0.2 293,050 - GFF3540 0.49 +0.1 293,069 - GFF3540 0.50 +1.1 293,250 + GFF3540 0.61 -0.8 293,875 - +0.6 293,914 - +0.8 294,035 + GFF3541 0.32 +0.4 294,036 - GFF3541 0.33 +0.0 294,119 - GFF3541 0.76 +0.0 294,152 + +0.1 294,189 - +0.1 294,252 - +1.1 294,266 + -0.2 294,596 + GFF3542 0.26 +0.3 294,616 + GFF3542 0.27 +0.6 294,716 - GFF3542 0.35 +0.0 294,839 + GFF3542 0.45 -0.1
Or see this region's nucleotide sequence