Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF323 and GFF324 are separated by 218 nucleotides GFF324 and GFF325 are separated by 10 nucleotides GFF325 and GFF326 are separated by 145 nucleotides GFF326 and GFF327 overlap by 4 nucleotides
GFF323 - Transcriptional regulatory protein zraR, at 37,731 to 40,529
GFF323
GFF324 - PTS system, mannose-specific IIA component, at 40,748 to 41,173
GFF324
GFF325 - PTS system, gluconate-specific IIB component (EC 2.7.1.69), at 41,184 to 41,669
GFF325
GFF326 - PTS system, mannose-specific IIC component, at 41,815 to 42,564
GFF326
GFF327 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 42,561 to 43,421
GFF327
Position (kb)
41
42 Strain fitness (log2 ratio)
-1
0
1 at 40.188 kb on - strand, within GFF323 at 40.223 kb on + strand, within GFF323 at 40.362 kb on - strand at 40.362 kb on - strand at 40.505 kb on + strand at 40.510 kb on - strand at 40.510 kb on - strand at 40.510 kb on - strand at 40.522 kb on - strand at 40.527 kb on + strand at 40.625 kb on + strand at 40.708 kb on - strand at 40.708 kb on - strand at 40.709 kb on + strand at 40.709 kb on + strand at 40.710 kb on - strand at 40.710 kb on - strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.829 kb on - strand, within GFF324 at 40.995 kb on + strand, within GFF324 at 41.148 kb on + strand at 41.322 kb on - strand, within GFF325 at 41.325 kb on + strand, within GFF325 at 41.464 kb on + strand, within GFF325 at 41.630 kb on + strand at 41.651 kb on + strand at 41.678 kb on + strand at 41.744 kb on + strand at 41.801 kb on + strand at 41.801 kb on + strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.809 kb on - strand at 41.814 kb on + strand at 41.825 kb on + strand at 41.852 kb on - strand at 41.852 kb on - strand at 41.881 kb on + strand at 41.904 kb on - strand, within GFF326 at 41.949 kb on + strand, within GFF326 at 41.993 kb on - strand, within GFF326 at 42.054 kb on + strand, within GFF326 at 42.055 kb on - strand, within GFF326 at 42.245 kb on - strand, within GFF326 at 42.577 kb on + strand at 42.577 kb on + strand at 42.578 kb on - strand at 42.578 kb on - strand at 42.654 kb on - strand, within GFF327
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 40,188 - GFF323 0.88 +0.0 40,223 + GFF323 0.89 -0.4 40,362 - -0.4 40,362 - -0.6 40,505 + +0.1 40,510 - +0.4 40,510 - -0.3 40,510 - -0.3 40,522 - +0.1 40,527 + -0.3 40,625 + +0.2 40,708 - -0.1 40,708 - +0.1 40,709 + -0.0 40,709 + +0.5 40,710 - -0.1 40,710 - -0.2 40,727 + +0.7 40,727 + -0.2 40,727 + +1.2 40,727 + +0.0 40,829 - GFF324 0.19 -0.3 40,995 + GFF324 0.58 +0.3 41,148 + -0.2 41,322 - GFF325 0.28 -0.2 41,325 + GFF325 0.29 -1.3 41,464 + GFF325 0.58 -0.5 41,630 + +0.2 41,651 + +0.0 41,678 + +0.1 41,744 + +0.4 41,801 + -0.0 41,801 + -0.0 41,803 + +0.1 41,803 + -0.4 41,809 - -0.3 41,814 + +0.3 41,825 + +0.0 41,852 - +0.2 41,852 - -0.7 41,881 + +1.2 41,904 - GFF326 0.12 -0.2 41,949 + GFF326 0.18 -0.1 41,993 - GFF326 0.24 -0.2 42,054 + GFF326 0.32 +0.2 42,055 - GFF326 0.32 -0.5 42,245 - GFF326 0.57 -0.4 42,577 + -0.4 42,577 + +0.4 42,578 - +0.2 42,578 - +0.3 42,654 - GFF327 0.11 +0.3
Or see this region's nucleotide sequence