Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF319 and GFF320 overlap by 23 nucleotides GFF320 and GFF321 overlap by 16 nucleotides GFF321 and GFF322 are separated by 71 nucleotides GFF322 and GFF323 are separated by 11 nucleotides GFF323 and GFF324 are separated by 218 nucleotides GFF324 and GFF325 are separated by 10 nucleotides
GFF319 - Beta-glucosidase (EC 3.2.1.21), at 35,753 to 37,135
GFF319
GFF320 - hypothetical protein, at 37,113 to 37,256
GFF320
GFF321 - Putative secreted protein, at 37,241 to 37,534
GFF321
GFF322 - hypothetical protein, at 37,606 to 37,719
GFF322
GFF323 - Transcriptional regulatory protein zraR, at 37,731 to 40,529
GFF323
GFF324 - PTS system, mannose-specific IIA component, at 40,748 to 41,173
GFF324
GFF325 - PTS system, gluconate-specific IIB component (EC 2.7.1.69), at 41,184 to 41,669
GFF325
Position (kb) 37
38
39
40
41 Strain fitness (log2 ratio) -1
0
1 at 36.785 kb on - strand, within GFF319 at 36.897 kb on - strand, within GFF319 at 36.924 kb on - strand, within GFF319 at 36.963 kb on + strand, within GFF319 at 37.067 kb on - strand at 37.281 kb on + strand, within GFF321 at 37.281 kb on + strand, within GFF321 at 37.281 kb on + strand, within GFF321 at 37.282 kb on - strand, within GFF321 at 37.282 kb on - strand, within GFF321 at 37.570 kb on + strand at 37.571 kb on + strand at 37.571 kb on + strand at 37.577 kb on + strand at 37.686 kb on + strand, within GFF322 at 37.686 kb on + strand, within GFF322 at 37.687 kb on - strand, within GFF322 at 37.691 kb on + strand, within GFF322 at 37.692 kb on - strand, within GFF322 at 37.729 kb on + strand at 37.729 kb on + strand at 37.729 kb on + strand at 37.730 kb on - strand at 37.740 kb on - strand at 37.929 kb on + strand at 38.035 kb on - strand, within GFF323 at 38.134 kb on + strand, within GFF323 at 38.226 kb on - strand, within GFF323 at 38.268 kb on - strand, within GFF323 at 38.336 kb on + strand, within GFF323 at 38.337 kb on - strand, within GFF323 at 38.370 kb on + strand, within GFF323 at 38.403 kb on + strand, within GFF323 at 38.429 kb on + strand, within GFF323 at 38.430 kb on - strand, within GFF323 at 38.430 kb on - strand, within GFF323 at 38.541 kb on - strand, within GFF323 at 38.563 kb on + strand, within GFF323 at 38.564 kb on - strand, within GFF323 at 38.686 kb on + strand, within GFF323 at 38.742 kb on - strand, within GFF323 at 38.858 kb on - strand, within GFF323 at 38.897 kb on + strand, within GFF323 at 38.898 kb on - strand, within GFF323 at 38.974 kb on - strand, within GFF323 at 38.995 kb on - strand, within GFF323 at 39.014 kb on + strand, within GFF323 at 39.014 kb on + strand, within GFF323 at 39.014 kb on + strand, within GFF323 at 39.085 kb on - strand, within GFF323 at 39.117 kb on + strand, within GFF323 at 39.163 kb on + strand, within GFF323 at 39.270 kb on + strand, within GFF323 at 39.356 kb on + strand, within GFF323 at 39.389 kb on - strand, within GFF323 at 39.502 kb on - strand, within GFF323 at 39.502 kb on - strand, within GFF323 at 39.624 kb on - strand, within GFF323 at 39.688 kb on + strand, within GFF323 at 39.963 kb on + strand, within GFF323 at 40.188 kb on - strand, within GFF323 at 40.223 kb on + strand, within GFF323 at 40.362 kb on - strand at 40.362 kb on - strand at 40.505 kb on + strand at 40.510 kb on - strand at 40.510 kb on - strand at 40.510 kb on - strand at 40.522 kb on - strand at 40.527 kb on + strand at 40.625 kb on + strand at 40.708 kb on - strand at 40.708 kb on - strand at 40.709 kb on + strand at 40.709 kb on + strand at 40.710 kb on - strand at 40.710 kb on - strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.727 kb on + strand at 40.829 kb on - strand, within GFF324 at 40.995 kb on + strand, within GFF324 at 41.148 kb on + strand at 41.322 kb on - strand, within GFF325 at 41.325 kb on + strand, within GFF325 at 41.464 kb on + strand, within GFF325
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 36,785 - GFF319 0.75 -0.2 36,897 - GFF319 0.83 +1.1 36,924 - GFF319 0.85 +0.2 36,963 + GFF319 0.87 -0.4 37,067 - -0.3 37,281 + GFF321 0.14 +0.0 37,281 + GFF321 0.14 -0.2 37,281 + GFF321 0.14 -0.2 37,282 - GFF321 0.14 +0.1 37,282 - GFF321 0.14 +0.2 37,570 + +0.7 37,571 + +0.4 37,571 + +0.5 37,577 + -0.9 37,686 + GFF322 0.70 +0.9 37,686 + GFF322 0.70 +0.1 37,687 - GFF322 0.71 -0.1 37,691 + GFF322 0.75 -0.0 37,692 - GFF322 0.75 +0.1 37,729 + +0.5 37,729 + +0.8 37,729 + -0.2 37,730 - +0.2 37,740 - +0.3 37,929 + +0.1 38,035 - GFF323 0.11 -0.1 38,134 + GFF323 0.14 -0.1 38,226 - GFF323 0.18 -0.2 38,268 - GFF323 0.19 -0.5 38,336 + GFF323 0.22 +0.0 38,337 - GFF323 0.22 -0.1 38,370 + GFF323 0.23 +0.3 38,403 + GFF323 0.24 +0.4 38,429 + GFF323 0.25 -0.7 38,430 - GFF323 0.25 +0.0 38,430 - GFF323 0.25 -1.0 38,541 - GFF323 0.29 +0.3 38,563 + GFF323 0.30 -0.6 38,564 - GFF323 0.30 +0.2 38,686 + GFF323 0.34 +0.1 38,742 - GFF323 0.36 -0.0 38,858 - GFF323 0.40 -0.4 38,897 + GFF323 0.42 -0.1 38,898 - GFF323 0.42 -0.2 38,974 - GFF323 0.44 -0.2 38,995 - GFF323 0.45 -0.1 39,014 + GFF323 0.46 -0.1 39,014 + GFF323 0.46 -0.1 39,014 + GFF323 0.46 +0.2 39,085 - GFF323 0.48 -0.4 39,117 + GFF323 0.50 -0.0 39,163 + GFF323 0.51 +0.2 39,270 + GFF323 0.55 -0.2 39,356 + GFF323 0.58 +0.3 39,389 - GFF323 0.59 +0.1 39,502 - GFF323 0.63 +0.1 39,502 - GFF323 0.63 -0.2 39,624 - GFF323 0.68 +0.0 39,688 + GFF323 0.70 -0.3 39,963 + GFF323 0.80 -0.1 40,188 - GFF323 0.88 +0.0 40,223 + GFF323 0.89 -0.4 40,362 - -0.4 40,362 - -0.6 40,505 + +0.1 40,510 - +0.4 40,510 - -0.3 40,510 - -0.3 40,522 - +0.1 40,527 + -0.3 40,625 + +0.2 40,708 - -0.1 40,708 - +0.1 40,709 + -0.0 40,709 + +0.5 40,710 - -0.1 40,710 - -0.2 40,727 + +0.7 40,727 + -0.2 40,727 + +1.2 40,727 + +0.0 40,829 - GFF324 0.19 -0.3 40,995 + GFF324 0.58 +0.3 41,148 + -0.2 41,322 - GFF325 0.28 -0.2 41,325 + GFF325 0.29 -1.3 41,464 + GFF325 0.58 -0.5
Or see this region's nucleotide sequence