Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF273 and GFF274 are separated by 189 nucleotides GFF274 and GFF275 overlap by 1 nucleotides GFF275 and GFF276 are separated by 38 nucleotides
GFF273 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 48,144 to 50,333
GFF273
GFF274 - Xaa-Pro dipeptidase PepQ (EC 3.4.13.9), at 50,523 to 51,854
GFF274
GFF275 - FIG000605: protein co-occurring with transport systems (COG1739), at 51,854 to 52,468
GFF275
GFF276 - Potassium uptake protein TrkH, at 52,507 to 53,958
GFF276
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-1
0
1 at 49.625 kb on + strand, within GFF273 at 49.913 kb on + strand, within GFF273 at 49.914 kb on - strand, within GFF273 at 50.032 kb on - strand, within GFF273 at 50.057 kb on + strand, within GFF273 at 50.058 kb on - strand, within GFF273 at 50.156 kb on + strand at 50.309 kb on - strand at 50.325 kb on - strand at 50.326 kb on + strand at 50.326 kb on + strand at 50.618 kb on + strand at 50.793 kb on - strand, within GFF274 at 50.982 kb on + strand, within GFF274 at 50.983 kb on - strand, within GFF274 at 50.991 kb on + strand, within GFF274 at 50.991 kb on + strand, within GFF274 at 50.992 kb on - strand, within GFF274 at 51.162 kb on + strand, within GFF274 at 51.207 kb on + strand, within GFF274 at 51.354 kb on - strand, within GFF274 at 51.412 kb on - strand, within GFF274 at 51.557 kb on - strand, within GFF274 at 51.557 kb on - strand, within GFF274 at 51.619 kb on - strand, within GFF274 at 51.684 kb on + strand, within GFF274 at 51.685 kb on - strand, within GFF274 at 51.685 kb on - strand, within GFF274 at 51.794 kb on + strand at 51.853 kb on - strand at 51.922 kb on + strand, within GFF275 at 51.923 kb on - strand, within GFF275 at 51.928 kb on - strand, within GFF275 at 51.948 kb on + strand, within GFF275 at 51.949 kb on - strand, within GFF275 at 52.160 kb on + strand, within GFF275 at 52.160 kb on + strand, within GFF275 at 52.160 kb on + strand, within GFF275 at 52.160 kb on + strand, within GFF275 at 52.160 kb on + strand, within GFF275 at 52.161 kb on - strand, within GFF275 at 52.161 kb on - strand, within GFF275 at 52.161 kb on - strand, within GFF275 at 52.168 kb on + strand, within GFF275 at 52.202 kb on + strand, within GFF275 at 52.228 kb on - strand, within GFF275 at 52.271 kb on - strand, within GFF275 at 52.271 kb on - strand, within GFF275 at 52.287 kb on - strand, within GFF275 at 52.318 kb on - strand, within GFF275 at 52.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 49,625 + GFF273 0.68 +0.4 49,913 + GFF273 0.81 +0.3 49,914 - GFF273 0.81 +0.0 50,032 - GFF273 0.86 +0.7 50,057 + GFF273 0.87 +0.2 50,058 - GFF273 0.87 +0.1 50,156 + -0.3 50,309 - +0.0 50,325 - +0.1 50,326 + -0.4 50,326 + +0.2 50,618 + -0.1 50,793 - GFF274 0.20 +0.5 50,982 + GFF274 0.34 +0.1 50,983 - GFF274 0.35 -0.3 50,991 + GFF274 0.35 +0.5 50,991 + GFF274 0.35 -0.8 50,992 - GFF274 0.35 +0.2 51,162 + GFF274 0.48 +0.0 51,207 + GFF274 0.51 -0.3 51,354 - GFF274 0.62 +0.3 51,412 - GFF274 0.67 +0.0 51,557 - GFF274 0.78 -0.1 51,557 - GFF274 0.78 +0.1 51,619 - GFF274 0.82 +1.0 51,684 + GFF274 0.87 +0.1 51,685 - GFF274 0.87 +0.1 51,685 - GFF274 0.87 -0.1 51,794 + +0.2 51,853 - +0.5 51,922 + GFF275 0.11 +0.1 51,923 - GFF275 0.11 +0.6 51,928 - GFF275 0.12 -0.2 51,948 + GFF275 0.15 -0.5 51,949 - GFF275 0.15 -0.2 52,160 + GFF275 0.50 -0.5 52,160 + GFF275 0.50 +0.0 52,160 + GFF275 0.50 -0.1 52,160 + GFF275 0.50 +0.1 52,160 + GFF275 0.50 +0.7 52,161 - GFF275 0.50 +0.2 52,161 - GFF275 0.50 -0.2 52,161 - GFF275 0.50 -0.0 52,168 + GFF275 0.51 +1.7 52,202 + GFF275 0.57 -0.3 52,228 - GFF275 0.61 +0.1 52,271 - GFF275 0.68 +0.1 52,271 - GFF275 0.68 +0.2 52,287 - GFF275 0.70 -0.2 52,318 - GFF275 0.75 +0.4 52,467 - +0.1
Or see this region's nucleotide sequence