Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2408 and GFF2409 are separated by 118 nucleotides GFF2409 and GFF2410 are separated by 10 nucleotides GFF2410 and GFF2411 are separated by 13 nucleotides
GFF2408 - GTP-binding protein EngA, at 19,941 to 21,413
GFF2408
GFF2409 - Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB), at 21,532 to 22,710
GFF2409
GFF2410 - Mlr7403 protein, at 22,721 to 23,341
GFF2410
GFF2411 - Histidyl-tRNA synthetase (EC 6.1.1.21), at 23,355 to 24,629
GFF2411
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.472 kb on - strand at 21.678 kb on - strand, within GFF2409 at 21.714 kb on - strand, within GFF2409 at 21.822 kb on - strand, within GFF2409 at 21.822 kb on - strand, within GFF2409 at 21.826 kb on - strand, within GFF2409 at 21.843 kb on + strand, within GFF2409 at 21.916 kb on - strand, within GFF2409 at 21.921 kb on + strand, within GFF2409 at 21.923 kb on + strand, within GFF2409 at 22.221 kb on + strand, within GFF2409 at 22.343 kb on + strand, within GFF2409 at 22.343 kb on + strand, within GFF2409 at 22.508 kb on - strand, within GFF2409 at 22.528 kb on - strand, within GFF2409 at 22.928 kb on - strand, within GFF2410 at 22.960 kb on + strand, within GFF2410 at 23.086 kb on - strand, within GFF2410 at 23.096 kb on - strand, within GFF2410
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 21,472 - -0.1 21,678 - GFF2409 0.12 +0.1 21,714 - GFF2409 0.15 -0.1 21,822 - GFF2409 0.25 -2.6 21,822 - GFF2409 0.25 -1.2 21,826 - GFF2409 0.25 +0.3 21,843 + GFF2409 0.26 -0.7 21,916 - GFF2409 0.33 -1.1 21,921 + GFF2409 0.33 -0.9 21,923 + GFF2409 0.33 -2.3 22,221 + GFF2409 0.58 -0.7 22,343 + GFF2409 0.69 -1.1 22,343 + GFF2409 0.69 -0.2 22,508 - GFF2409 0.83 -0.5 22,528 - GFF2409 0.84 -0.8 22,928 - GFF2410 0.33 +0.6 22,960 + GFF2410 0.38 +0.0 23,086 - GFF2410 0.59 -0.6 23,096 - GFF2410 0.60 -0.1
Or see this region's nucleotide sequence