Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2235 and GFF2236 are separated by 15 nucleotides GFF2236 and GFF2237 are separated by 22 nucleotides
GFF2235 - Aldose-ketose isomerase YihS, at 30,530 to 31,771
GFF2235
GFF2236 - Aldolase YihT, at 31,787 to 32,665
GFF2236
GFF2237 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31), at 32,688 to 33,584
GFF2237
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.843 kb on - strand, within GFF2235 at 30.848 kb on - strand, within GFF2235 at 30.853 kb on + strand, within GFF2235 at 31.072 kb on + strand, within GFF2235 at 31.073 kb on - strand, within GFF2235 at 31.081 kb on + strand, within GFF2235 at 31.082 kb on - strand, within GFF2235 at 31.218 kb on + strand, within GFF2235 at 31.249 kb on + strand, within GFF2235 at 31.338 kb on + strand, within GFF2235 at 31.356 kb on - strand, within GFF2235 at 31.460 kb on + strand, within GFF2235 at 31.557 kb on + strand, within GFF2235 at 31.558 kb on - strand, within GFF2235 at 31.859 kb on + strand at 31.859 kb on + strand at 32.015 kb on - strand, within GFF2236 at 32.092 kb on - strand, within GFF2236 at 32.180 kb on + strand, within GFF2236 at 32.181 kb on - strand, within GFF2236 at 32.222 kb on + strand, within GFF2236 at 32.336 kb on - strand, within GFF2236 at 32.455 kb on - strand, within GFF2236 at 32.491 kb on - strand, within GFF2236 at 32.655 kb on + strand at 32.656 kb on - strand at 32.680 kb on + strand at 32.680 kb on + strand at 32.681 kb on - strand at 32.788 kb on + strand, within GFF2237 at 32.976 kb on + strand, within GFF2237 at 33.051 kb on - strand, within GFF2237 at 33.058 kb on + strand, within GFF2237 at 33.058 kb on + strand, within GFF2237 at 33.059 kb on - strand, within GFF2237 at 33.059 kb on - strand, within GFF2237 at 33.153 kb on + strand, within GFF2237 at 33.305 kb on + strand, within GFF2237 at 33.305 kb on + strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.306 kb on - strand, within GFF2237 at 33.573 kb on + strand at 33.576 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 30,843 - GFF2235 0.25 -0.1 30,848 - GFF2235 0.26 -0.1 30,853 + GFF2235 0.26 -0.3 31,072 + GFF2235 0.44 -0.1 31,073 - GFF2235 0.44 -0.2 31,081 + GFF2235 0.44 -1.1 31,082 - GFF2235 0.44 +0.1 31,218 + GFF2235 0.55 +1.2 31,249 + GFF2235 0.58 -0.1 31,338 + GFF2235 0.65 -0.1 31,356 - GFF2235 0.67 +0.2 31,460 + GFF2235 0.75 -0.1 31,557 + GFF2235 0.83 +0.5 31,558 - GFF2235 0.83 +0.7 31,859 + +0.1 31,859 + +0.1 32,015 - GFF2236 0.26 +0.7 32,092 - GFF2236 0.35 +0.0 32,180 + GFF2236 0.45 +0.2 32,181 - GFF2236 0.45 +0.4 32,222 + GFF2236 0.49 +0.3 32,336 - GFF2236 0.62 +0.0 32,455 - GFF2236 0.76 +0.2 32,491 - GFF2236 0.80 +0.6 32,655 + -0.2 32,656 - -0.3 32,680 + +1.3 32,680 + +0.5 32,681 - -0.2 32,788 + GFF2237 0.11 +0.3 32,976 + GFF2237 0.32 +0.3 33,051 - GFF2237 0.40 -0.0 33,058 + GFF2237 0.41 +0.3 33,058 + GFF2237 0.41 +0.1 33,059 - GFF2237 0.41 -0.8 33,059 - GFF2237 0.41 +0.2 33,153 + GFF2237 0.52 -1.9 33,305 + GFF2237 0.69 +0.1 33,305 + GFF2237 0.69 -0.0 33,306 - GFF2237 0.69 -1.8 33,306 - GFF2237 0.69 +0.0 33,306 - GFF2237 0.69 -0.2 33,306 - GFF2237 0.69 +0.0 33,306 - GFF2237 0.69 -0.3 33,573 + -0.2 33,576 - +0.3
Or see this region's nucleotide sequence