Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2219 and GFF2220 are separated by 312 nucleotides GFF2220 and GFF2221 are separated by 8 nucleotides
GFF2219 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 10,095 to 11,468
GFF2219
GFF2220 - Nitrogen regulation protein NR(I), at 11,781 to 13,190
GFF2220
GFF2221 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 13,199 to 14,248
GFF2221
Position (kb)
11
12
13
14 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.815 kb on - strand, within GFF2219 at 10.815 kb on - strand, within GFF2219 at 10.818 kb on + strand, within GFF2219 at 10.818 kb on + strand, within GFF2219 at 10.819 kb on - strand, within GFF2219 at 10.819 kb on - strand, within GFF2219 at 10.899 kb on - strand, within GFF2219 at 11.100 kb on + strand, within GFF2219 at 11.101 kb on - strand, within GFF2219 at 11.289 kb on + strand, within GFF2219 at 11.312 kb on - strand, within GFF2219 at 11.348 kb on + strand at 11.657 kb on - strand at 11.669 kb on - strand at 11.720 kb on + strand at 11.754 kb on - strand at 11.878 kb on - strand at 11.977 kb on - strand, within GFF2220 at 12.063 kb on + strand, within GFF2220 at 12.064 kb on - strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.406 kb on + strand, within GFF2220 at 12.407 kb on - strand, within GFF2220 at 12.639 kb on - strand, within GFF2220 at 12.704 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.731 kb on + strand, within GFF2220 at 12.769 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.804 kb on - strand, within GFF2220 at 12.826 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.869 kb on + strand, within GFF2220 at 12.870 kb on - strand, within GFF2220 at 12.930 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.950 kb on + strand, within GFF2220 at 12.983 kb on + strand, within GFF2220 at 12.984 kb on - strand, within GFF2220 at 13.101 kb on - strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.154 kb on + strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.155 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.191 kb on - strand at 13.298 kb on - strand at 13.298 kb on - strand at 13.301 kb on + strand at 13.403 kb on - strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.516 kb on + strand, within GFF2221 at 13.517 kb on - strand, within GFF2221 at 13.526 kb on + strand, within GFF2221 at 13.579 kb on - strand, within GFF2221 at 13.649 kb on - strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.652 kb on + strand, within GFF2221 at 13.653 kb on - strand, within GFF2221 at 13.715 kb on + strand, within GFF2221 at 13.716 kb on - strand, within GFF2221 at 13.750 kb on - strand, within GFF2221 at 13.787 kb on + strand, within GFF2221 at 13.937 kb on + strand, within GFF2221 at 13.938 kb on - strand, within GFF2221 at 13.939 kb on + strand, within GFF2221 at 13.939 kb on + strand, within GFF2221
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 10,815 - GFF2219 0.52 -0.2 10,815 - GFF2219 0.52 -0.2 10,818 + GFF2219 0.53 +0.2 10,818 + GFF2219 0.53 -0.4 10,819 - GFF2219 0.53 -0.5 10,819 - GFF2219 0.53 -0.1 10,899 - GFF2219 0.59 +0.1 11,100 + GFF2219 0.73 +0.1 11,101 - GFF2219 0.73 +0.2 11,289 + GFF2219 0.87 -0.3 11,312 - GFF2219 0.89 -0.1 11,348 + -0.5 11,657 - +0.7 11,669 - +0.1 11,720 + -0.1 11,754 - +1.0 11,878 - -1.0 11,977 - GFF2220 0.14 -2.2 12,063 + GFF2220 0.20 -0.5 12,064 - GFF2220 0.20 -1.6 12,406 + GFF2220 0.44 -0.9 12,406 + GFF2220 0.44 -1.1 12,407 - GFF2220 0.44 -2.1 12,639 - GFF2220 0.61 -0.8 12,704 + GFF2220 0.65 -0.3 12,731 + GFF2220 0.67 -1.0 12,731 + GFF2220 0.67 -1.4 12,769 - GFF2220 0.70 -0.6 12,804 - GFF2220 0.73 -2.0 12,804 - GFF2220 0.73 -0.2 12,826 + GFF2220 0.74 -1.7 12,869 + GFF2220 0.77 -0.7 12,869 + GFF2220 0.77 -0.5 12,870 - GFF2220 0.77 -0.6 12,930 + GFF2220 0.81 +0.4 12,950 + GFF2220 0.83 -1.5 12,950 + GFF2220 0.83 +0.7 12,983 + GFF2220 0.85 -0.1 12,984 - GFF2220 0.85 -0.5 13,101 - -0.4 13,154 + -1.8 13,154 + -1.3 13,154 + -1.0 13,154 + +0.1 13,155 - -0.9 13,155 - -0.3 13,155 - -0.6 13,191 - +0.4 13,191 - +0.7 13,191 - +0.7 13,298 - -0.1 13,298 - -0.6 13,301 + -1.0 13,403 - GFF2221 0.19 +0.4 13,516 + GFF2221 0.30 -1.5 13,516 + GFF2221 0.30 +0.2 13,516 + GFF2221 0.30 -1.7 13,516 + GFF2221 0.30 -0.9 13,517 - GFF2221 0.30 +2.1 13,526 + GFF2221 0.31 -1.3 13,579 - GFF2221 0.36 +0.4 13,649 - GFF2221 0.43 -0.4 13,652 + GFF2221 0.43 -0.7 13,652 + GFF2221 0.43 -0.7 13,652 + GFF2221 0.43 -1.7 13,653 - GFF2221 0.43 +0.0 13,715 + GFF2221 0.49 -1.1 13,716 - GFF2221 0.49 -0.4 13,750 - GFF2221 0.52 -0.6 13,787 + GFF2221 0.56 -0.8 13,937 + GFF2221 0.70 -1.1 13,938 - GFF2221 0.70 +0.1 13,939 + GFF2221 0.70 -0.6 13,939 + GFF2221 0.70 -2.0
Or see this region's nucleotide sequence