Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2148 and GFF2149 are separated by 413 nucleotides GFF2149 and GFF2150 are separated by 119 nucleotides GFF2150 and GFF2151 overlap by 14 nucleotides
GFF2148 - Arginine pathway regulatory protein ArgR, repressor of arg regulon, at 38,421 to 38,891
GFF2148
GFF2149 - Malate dehydrogenase (EC 1.1.1.37), at 39,305 to 40,243
GFF2149
GFF2150 - Transcriptional regulator, GntR family, at 40,363 to 40,992
GFF2150
GFF2151 - Transcriptional regulator, GntR family, at 40,979 to 41,644
GFF2151
Position (kb)
39
40
41 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 38.502 kb on - strand, within GFF2148 at 38.505 kb on - strand, within GFF2148 at 38.532 kb on - strand, within GFF2148 at 38.862 kb on + strand at 38.891 kb on + strand at 38.905 kb on + strand at 38.926 kb on - strand at 38.982 kb on - strand at 39.103 kb on + strand at 39.103 kb on + strand at 39.106 kb on + strand at 39.111 kb on + strand at 39.113 kb on + strand at 39.114 kb on - strand at 39.164 kb on - strand at 39.180 kb on + strand at 39.181 kb on - strand at 39.248 kb on - strand at 39.278 kb on - strand at 39.281 kb on + strand at 39.282 kb on - strand at 39.290 kb on - strand at 39.293 kb on - strand at 39.357 kb on + strand at 39.520 kb on - strand, within GFF2149 at 39.577 kb on + strand, within GFF2149 at 39.769 kb on + strand, within GFF2149 at 40.062 kb on - strand, within GFF2149 at 40.082 kb on + strand, within GFF2149 at 40.082 kb on + strand, within GFF2149 at 40.083 kb on - strand, within GFF2149 at 40.139 kb on - strand, within GFF2149 at 40.237 kb on + strand at 40.237 kb on + strand at 40.241 kb on + strand at 40.315 kb on + strand at 40.315 kb on + strand at 40.402 kb on - strand at 40.518 kb on - strand, within GFF2150 at 40.544 kb on + strand, within GFF2150 at 40.683 kb on - strand, within GFF2150 at 40.753 kb on + strand, within GFF2150 at 40.804 kb on + strand, within GFF2150 at 40.804 kb on + strand, within GFF2150 at 40.813 kb on + strand, within GFF2150 at 40.814 kb on - strand, within GFF2150 at 40.819 kb on - strand, within GFF2150 at 40.917 kb on + strand, within GFF2150 at 40.963 kb on - strand at 40.963 kb on - strand at 41.047 kb on + strand, within GFF2151 at 41.070 kb on + strand, within GFF2151 at 41.090 kb on - strand, within GFF2151 at 41.094 kb on + strand, within GFF2151 at 41.095 kb on - strand, within GFF2151
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 38,502 - GFF2148 0.17 -0.2 38,505 - GFF2148 0.18 +0.9 38,532 - GFF2148 0.24 -0.4 38,862 + -1.1 38,891 + -2.6 38,905 + +0.5 38,926 - +0.3 38,982 - -0.3 39,103 + -0.0 39,103 + -0.0 39,106 + -0.4 39,111 + -0.8 39,113 + -0.4 39,114 - -0.3 39,164 - +0.5 39,180 + -0.3 39,181 - -0.6 39,248 - -1.3 39,278 - -1.1 39,281 + -0.5 39,282 - -0.3 39,290 - -0.7 39,293 - -1.2 39,357 + -0.6 39,520 - GFF2149 0.23 -1.5 39,577 + GFF2149 0.29 -0.9 39,769 + GFF2149 0.49 -1.4 40,062 - GFF2149 0.81 -1.5 40,082 + GFF2149 0.83 -0.4 40,082 + GFF2149 0.83 -3.1 40,083 - GFF2149 0.83 -0.5 40,139 - GFF2149 0.89 -0.9 40,237 + +0.6 40,237 + +0.1 40,241 + -0.1 40,315 + -0.1 40,315 + -0.3 40,402 - -0.2 40,518 - GFF2150 0.25 +0.1 40,544 + GFF2150 0.29 -2.1 40,683 - GFF2150 0.51 +0.1 40,753 + GFF2150 0.62 +0.6 40,804 + GFF2150 0.70 -0.1 40,804 + GFF2150 0.70 -0.4 40,813 + GFF2150 0.71 -0.6 40,814 - GFF2150 0.72 +0.5 40,819 - GFF2150 0.72 -0.3 40,917 + GFF2150 0.88 -0.2 40,963 - +0.2 40,963 - -0.1 41,047 + GFF2151 0.10 -0.1 41,070 + GFF2151 0.14 +1.2 41,090 - GFF2151 0.17 -0.2 41,094 + GFF2151 0.17 -0.3 41,095 - GFF2151 0.17 +0.1
Or see this region's nucleotide sequence