Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1932 and GFF1933 are separated by 149 nucleotides GFF1933 and GFF1934 are separated by 48 nucleotides GFF1934 and GFF1935 overlap by 1 nucleotides
GFF1932 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 125,080 to 125,994
GFF1932
GFF1933 - DedA protein, at 126,144 to 126,803
GFF1933
GFF1934 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 126,852 to 127,664
GFF1934
GFF1935 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 127,664 to 128,677
GFF1935
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 126.032 kb on - strand at 126.138 kb on + strand at 126.240 kb on - strand, within GFF1933 at 126.327 kb on - strand, within GFF1933 at 126.327 kb on - strand, within GFF1933 at 126.327 kb on - strand, within GFF1933 at 126.328 kb on + strand, within GFF1933 at 126.786 kb on - strand at 126.853 kb on + strand at 127.047 kb on - strand, within GFF1934 at 127.086 kb on - strand, within GFF1934 at 127.099 kb on + strand, within GFF1934 at 127.247 kb on - strand, within GFF1934 at 127.273 kb on - strand, within GFF1934 at 127.298 kb on - strand, within GFF1934 at 127.305 kb on - strand, within GFF1934 at 127.357 kb on - strand, within GFF1934 at 127.514 kb on + strand, within GFF1934 at 127.515 kb on - strand, within GFF1934 at 127.598 kb on - strand at 127.675 kb on - strand at 127.675 kb on - strand at 127.799 kb on - strand, within GFF1935 at 127.868 kb on - strand, within GFF1935 at 127.953 kb on + strand, within GFF1935 at 128.032 kb on + strand, within GFF1935 at 128.084 kb on - strand, within GFF1935 at 128.206 kb on + strand, within GFF1935 at 128.306 kb on - strand, within GFF1935 at 128.397 kb on - strand, within GFF1935
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 126,032 - -1.5 126,138 + +0.3 126,240 - GFF1933 0.15 -0.4 126,327 - GFF1933 0.28 -0.1 126,327 - GFF1933 0.28 +0.2 126,327 - GFF1933 0.28 -0.0 126,328 + GFF1933 0.28 -0.2 126,786 - +0.8 126,853 + -0.0 127,047 - GFF1934 0.24 -1.5 127,086 - GFF1934 0.29 -0.8 127,099 + GFF1934 0.30 -1.8 127,247 - GFF1934 0.49 -0.6 127,273 - GFF1934 0.52 -0.2 127,298 - GFF1934 0.55 -1.9 127,305 - GFF1934 0.56 -1.8 127,357 - GFF1934 0.62 -0.5 127,514 + GFF1934 0.81 -2.6 127,515 - GFF1934 0.82 -1.2 127,598 - +0.7 127,675 - -0.5 127,675 - -1.5 127,799 - GFF1935 0.13 -0.0 127,868 - GFF1935 0.20 +0.3 127,953 + GFF1935 0.29 -1.1 128,032 + GFF1935 0.36 -1.7 128,084 - GFF1935 0.41 +0.4 128,206 + GFF1935 0.53 -0.7 128,306 - GFF1935 0.63 +0.5 128,397 - GFF1935 0.72 -1.5
Or see this region's nucleotide sequence