Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1889 and GFF1890 are separated by 86 nucleotides GFF1890 and GFF1891 are separated by 15 nucleotides GFF1891 and GFF1892 are separated by 394 nucleotides
GFF1889 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 82,881 to 84,683
GFF1889
GFF1890 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 84,770 to 85,432
GFF1890
GFF1891 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 85,448 to 85,891
GFF1891
GFF1892 - hypothetical protein, at 86,286 to 86,438
GFF1892
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 83.824 kb on + strand, within GFF1889 at 83.855 kb on - strand, within GFF1889 at 83.871 kb on - strand, within GFF1889 at 83.881 kb on + strand, within GFF1889 at 83.881 kb on + strand, within GFF1889 at 83.882 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.886 kb on - strand, within GFF1889 at 83.985 kb on - strand, within GFF1889 at 83.985 kb on - strand, within GFF1889 at 84.038 kb on + strand, within GFF1889 at 84.039 kb on - strand, within GFF1889 at 84.039 kb on - strand, within GFF1889 at 84.274 kb on + strand, within GFF1889 at 84.354 kb on - strand, within GFF1889 at 84.493 kb on - strand, within GFF1889 at 84.660 kb on + strand at 84.937 kb on + strand, within GFF1890 at 84.938 kb on - strand, within GFF1890 at 84.940 kb on - strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.972 kb on + strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.973 kb on - strand, within GFF1890 at 84.994 kb on + strand, within GFF1890 at 84.994 kb on + strand, within GFF1890 at 85.027 kb on - strand, within GFF1890 at 85.093 kb on - strand, within GFF1890 at 85.235 kb on + strand, within GFF1890 at 85.235 kb on + strand, within GFF1890 at 85.236 kb on - strand, within GFF1890 at 85.239 kb on + strand, within GFF1890 at 85.240 kb on - strand, within GFF1890 at 85.240 kb on - strand, within GFF1890 at 85.410 kb on - strand at 85.435 kb on + strand at 85.435 kb on + strand at 85.545 kb on + strand, within GFF1891 at 85.641 kb on + strand, within GFF1891 at 85.840 kb on + strand, within GFF1891 at 85.885 kb on + strand at 85.896 kb on + strand at 86.083 kb on + strand at 86.083 kb on + strand at 86.109 kb on - strand at 86.191 kb on + strand at 86.203 kb on - strand at 86.246 kb on + strand at 86.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 83,824 + GFF1889 0.52 -1.5 83,855 - GFF1889 0.54 -1.7 83,871 - GFF1889 0.55 -0.7 83,881 + GFF1889 0.55 -0.5 83,881 + GFF1889 0.55 -0.6 83,882 - GFF1889 0.56 -1.0 83,886 - GFF1889 0.56 -1.1 83,886 - GFF1889 0.56 -1.8 83,886 - GFF1889 0.56 -2.0 83,985 - GFF1889 0.61 -1.2 83,985 - GFF1889 0.61 -1.8 84,038 + GFF1889 0.64 -1.5 84,039 - GFF1889 0.64 -1.0 84,039 - GFF1889 0.64 -1.2 84,274 + GFF1889 0.77 -0.4 84,354 - GFF1889 0.82 -0.2 84,493 - GFF1889 0.89 -1.1 84,660 + -1.0 84,937 + GFF1890 0.25 -1.2 84,938 - GFF1890 0.25 -0.1 84,940 - GFF1890 0.26 -0.9 84,972 + GFF1890 0.30 -1.5 84,972 + GFF1890 0.30 +0.1 84,972 + GFF1890 0.30 -1.3 84,973 - GFF1890 0.31 -1.3 84,973 - GFF1890 0.31 -1.4 84,973 - GFF1890 0.31 -1.3 84,994 + GFF1890 0.34 -0.9 84,994 + GFF1890 0.34 -1.1 85,027 - GFF1890 0.39 -1.1 85,093 - GFF1890 0.49 -0.6 85,235 + GFF1890 0.70 -0.3 85,235 + GFF1890 0.70 -1.2 85,236 - GFF1890 0.70 -0.7 85,239 + GFF1890 0.71 -1.2 85,240 - GFF1890 0.71 -0.9 85,240 - GFF1890 0.71 -1.4 85,410 - -1.4 85,435 + +0.7 85,435 + -1.2 85,545 + GFF1891 0.22 -1.6 85,641 + GFF1891 0.43 -2.1 85,840 + GFF1891 0.88 -2.5 85,885 + -3.6 85,896 + -1.3 86,083 + -1.2 86,083 + -1.3 86,109 - +0.8 86,191 + +0.0 86,203 - -0.3 86,246 + +0.0 86,426 + +0.2
Or see this region's nucleotide sequence