Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1884 and GFF1885 are separated by 14 nucleotides GFF1885 and GFF1886 overlap by 4 nucleotides GFF1886 and GFF1887 are separated by 30 nucleotides
GFF1884 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 76,756 to 77,298
GFF1884
GFF1885 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 77,313 to 78,290
GFF1885
GFF1886 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 78,287 to 81,013
GFF1886
GFF1887 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 81,044 to 82,381
GFF1887
Position (kb)
78
79
80
81
82 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 77.400 kb on + strand at 77.401 kb on - strand at 77.411 kb on - strand, within GFF1885 at 77.566 kb on + strand, within GFF1885 at 77.766 kb on - strand, within GFF1885 at 77.769 kb on - strand, within GFF1885 at 77.824 kb on + strand, within GFF1885 at 77.870 kb on - strand, within GFF1885 at 77.975 kb on + strand, within GFF1885 at 78.152 kb on - strand, within GFF1885 at 78.429 kb on + strand at 78.429 kb on + strand at 78.512 kb on - strand at 78.567 kb on + strand, within GFF1886 at 78.567 kb on + strand, within GFF1886 at 78.568 kb on - strand, within GFF1886 at 78.568 kb on - strand, within GFF1886 at 78.580 kb on + strand, within GFF1886 at 78.580 kb on + strand, within GFF1886 at 79.082 kb on - strand, within GFF1886 at 79.082 kb on - strand, within GFF1886 at 79.099 kb on - strand, within GFF1886 at 79.099 kb on - strand, within GFF1886 at 79.578 kb on - strand, within GFF1886 at 79.578 kb on - strand, within GFF1886 at 79.612 kb on + strand, within GFF1886 at 79.612 kb on + strand, within GFF1886 at 79.612 kb on + strand, within GFF1886 at 79.757 kb on - strand, within GFF1886 at 79.757 kb on - strand, within GFF1886 at 79.929 kb on + strand, within GFF1886 at 80.001 kb on + strand, within GFF1886 at 80.199 kb on + strand, within GFF1886 at 80.199 kb on + strand, within GFF1886 at 80.199 kb on + strand, within GFF1886 at 80.200 kb on - strand, within GFF1886 at 80.309 kb on - strand, within GFF1886 at 80.476 kb on - strand, within GFF1886 at 80.665 kb on + strand, within GFF1886 at 80.786 kb on - strand at 80.791 kb on + strand at 80.919 kb on + strand at 80.927 kb on - strand at 80.927 kb on - strand at 80.927 kb on - strand at 80.955 kb on - strand at 81.145 kb on + strand at 81.145 kb on + strand at 81.319 kb on + strand, within GFF1887 at 81.319 kb on + strand, within GFF1887 at 81.960 kb on - strand, within GFF1887 at 81.974 kb on - strand, within GFF1887 at 81.996 kb on + strand, within GFF1887 at 81.997 kb on - strand, within GFF1887 at 81.997 kb on - strand, within GFF1887
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 77,400 + -1.8 77,401 - -0.7 77,411 - GFF1885 0.10 -1.5 77,566 + GFF1885 0.26 -1.1 77,766 - GFF1885 0.46 -1.2 77,769 - GFF1885 0.47 -1.2 77,824 + GFF1885 0.52 -0.5 77,870 - GFF1885 0.57 -0.9 77,975 + GFF1885 0.68 -1.2 78,152 - GFF1885 0.86 -0.5 78,429 + -1.7 78,429 + -1.5 78,512 - -1.0 78,567 + GFF1886 0.10 -0.0 78,567 + GFF1886 0.10 -2.6 78,568 - GFF1886 0.10 -1.7 78,568 - GFF1886 0.10 -1.2 78,580 + GFF1886 0.11 -2.2 78,580 + GFF1886 0.11 -2.4 79,082 - GFF1886 0.29 -2.6 79,082 - GFF1886 0.29 -1.6 79,099 - GFF1886 0.30 -1.1 79,099 - GFF1886 0.30 -1.2 79,578 - GFF1886 0.47 -0.6 79,578 - GFF1886 0.47 -0.6 79,612 + GFF1886 0.49 -1.9 79,612 + GFF1886 0.49 -2.3 79,612 + GFF1886 0.49 -1.1 79,757 - GFF1886 0.54 +0.7 79,757 - GFF1886 0.54 -2.3 79,929 + GFF1886 0.60 -0.6 80,001 + GFF1886 0.63 -1.6 80,199 + GFF1886 0.70 -2.0 80,199 + GFF1886 0.70 -0.8 80,199 + GFF1886 0.70 -0.7 80,200 - GFF1886 0.70 -1.4 80,309 - GFF1886 0.74 -0.2 80,476 - GFF1886 0.80 -0.9 80,665 + GFF1886 0.87 -2.8 80,786 - -0.6 80,791 + -1.2 80,919 + -0.4 80,927 - -0.8 80,927 - -2.2 80,927 - -1.2 80,955 - -0.5 81,145 + -0.9 81,145 + +0.1 81,319 + GFF1887 0.21 +0.1 81,319 + GFF1887 0.21 -2.3 81,960 - GFF1887 0.68 -2.8 81,974 - GFF1887 0.70 -1.7 81,996 + GFF1887 0.71 -1.6 81,997 - GFF1887 0.71 -1.6 81,997 - GFF1887 0.71 -1.0
Or see this region's nucleotide sequence