Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1871 and GFF1872 are separated by 100 nucleotides GFF1872 and GFF1873 are separated by 54 nucleotides GFF1873 and GFF1874 are separated by 129 nucleotides GFF1874 and GFF1875 are separated by 81 nucleotides
GFF1871 - Menaquinone-specific isochorismate synthase (EC 5.4.4.2), at 63,775 to 65,070
GFF1871
GFF1872 - ElaB protein, at 65,171 to 65,476
GFF1872
GFF1873 - ElaA protein, at 65,531 to 65,992
GFF1873
GFF1874 - hypothetical protein, at 66,122 to 66,259
GFF1874
GFF1875 - Ribonuclease Z (EC 3.1.26.11), at 66,341 to 66,970
GFF1875
Position (kb) 65
66 Strain fitness (log2 ratio) -1
0
1 at 64.623 kb on + strand, within GFF1871 at 64.624 kb on - strand, within GFF1871 at 64.699 kb on + strand, within GFF1871 at 64.726 kb on - strand, within GFF1871 at 64.753 kb on + strand, within GFF1871 at 64.754 kb on - strand, within GFF1871 at 64.754 kb on - strand, within GFF1871 at 64.757 kb on + strand, within GFF1871 at 64.855 kb on + strand, within GFF1871 at 64.910 kb on + strand, within GFF1871 at 65.061 kb on + strand at 65.138 kb on - strand at 65.228 kb on - strand, within GFF1872 at 65.289 kb on + strand, within GFF1872 at 65.296 kb on - strand, within GFF1872 at 65.371 kb on - strand, within GFF1872 at 65.403 kb on - strand, within GFF1872 at 65.470 kb on - strand at 65.508 kb on + strand at 65.509 kb on - strand at 65.512 kb on + strand at 65.513 kb on - strand at 65.513 kb on - strand at 65.655 kb on + strand, within GFF1873 at 65.655 kb on + strand, within GFF1873 at 65.657 kb on + strand, within GFF1873 at 65.843 kb on - strand, within GFF1873 at 65.862 kb on - strand, within GFF1873 at 66.123 kb on + strand at 66.124 kb on - strand at 66.200 kb on - strand, within GFF1874 at 66.283 kb on - strand at 66.324 kb on + strand at 66.326 kb on + strand at 66.641 kb on + strand, within GFF1875 at 66.668 kb on + strand, within GFF1875 at 66.865 kb on + strand, within GFF1875 at 66.865 kb on + strand, within GFF1875 at 66.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 64,623 + GFF1871 0.65 +0.4 64,624 - GFF1871 0.66 -0.1 64,699 + GFF1871 0.71 -0.1 64,726 - GFF1871 0.73 -0.0 64,753 + GFF1871 0.75 +0.6 64,754 - GFF1871 0.76 -0.1 64,754 - GFF1871 0.76 +0.8 64,757 + GFF1871 0.76 -0.4 64,855 + GFF1871 0.83 -0.2 64,910 + GFF1871 0.88 +1.0 65,061 + -0.1 65,138 - +0.4 65,228 - GFF1872 0.19 -0.1 65,289 + GFF1872 0.39 +0.4 65,296 - GFF1872 0.41 -0.1 65,371 - GFF1872 0.65 +1.5 65,403 - GFF1872 0.76 -0.1 65,470 - +1.6 65,508 + +0.2 65,509 - +0.0 65,512 + -0.3 65,513 - +0.7 65,513 - +0.7 65,655 + GFF1873 0.27 +0.7 65,655 + GFF1873 0.27 -0.3 65,657 + GFF1873 0.27 +0.2 65,843 - GFF1873 0.68 +0.5 65,862 - GFF1873 0.72 +0.3 66,123 + +0.3 66,124 - +0.6 66,200 - GFF1874 0.57 +0.6 66,283 - -0.6 66,324 + +0.8 66,326 + +0.4 66,641 + GFF1875 0.48 +0.2 66,668 + GFF1875 0.52 +1.3 66,865 + GFF1875 0.83 +0.1 66,865 + GFF1875 0.83 +0.4 66,969 - -0.7
Or see this region's nucleotide sequence