Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1748 and GFF1749 are separated by 15 nucleotides GFF1749 and GFF1750 are separated by 255 nucleotides GFF1750 and GFF1751 are separated by 141 nucleotides
GFF1748 - Integrase, at 48,786 to 49,496
GFF1748
GFF1749 - Integrase, at 49,512 to 50,006
GFF1749
GFF1750 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 50,262 to 51,626
GFF1750
GFF1751 - Inner membrane protein translocase component YidC, long form, at 51,768 to 53,414
GFF1751
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 49.292 kb on + strand, within GFF1748 at 49.350 kb on - strand, within GFF1748 at 49.357 kb on + strand, within GFF1748 at 49.358 kb on - strand, within GFF1748 at 49.428 kb on - strand at 49.428 kb on - strand at 49.429 kb on + strand at 49.442 kb on + strand at 49.487 kb on - strand at 49.502 kb on + strand at 50.018 kb on - strand at 50.019 kb on + strand at 50.041 kb on - strand at 50.194 kb on + strand at 50.219 kb on - strand at 50.263 kb on + strand at 50.394 kb on + strand at 50.397 kb on + strand at 50.397 kb on + strand at 50.448 kb on + strand, within GFF1750 at 50.449 kb on - strand, within GFF1750 at 50.612 kb on + strand, within GFF1750 at 50.624 kb on + strand, within GFF1750 at 50.629 kb on - strand, within GFF1750 at 50.826 kb on - strand, within GFF1750 at 51.137 kb on + strand, within GFF1750 at 51.137 kb on + strand, within GFF1750 at 51.385 kb on - strand, within GFF1750
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 49,292 + GFF1748 0.71 +0.2 49,350 - GFF1748 0.79 +0.1 49,357 + GFF1748 0.80 -0.3 49,358 - GFF1748 0.80 +0.2 49,428 - +0.4 49,428 - -0.0 49,429 + +0.0 49,442 + +0.2 49,487 - +1.1 49,502 + +0.5 50,018 - -0.1 50,019 + -0.4 50,041 - +0.6 50,194 + +0.3 50,219 - +0.3 50,263 + -1.8 50,394 + -1.8 50,397 + -2.1 50,397 + -0.8 50,448 + GFF1750 0.14 -1.1 50,449 - GFF1750 0.14 -0.8 50,612 + GFF1750 0.26 -1.6 50,624 + GFF1750 0.27 -3.2 50,629 - GFF1750 0.27 -2.4 50,826 - GFF1750 0.41 -1.1 51,137 + GFF1750 0.64 -1.3 51,137 + GFF1750 0.64 -1.2 51,385 - GFF1750 0.82 -0.8
Or see this region's nucleotide sequence