Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1656 and GFF1657 are separated by 188 nucleotides GFF1657 and GFF1658 are separated by 53 nucleotides
GFF1656 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 39,018 to 40,751
GFF1656
GFF1657 - Penicillin-binding protein 2 (PBP-2), at 40,940 to 42,811
GFF1657
GFF1658 - Rhamnogalacturonides degradation protein RhiN, at 42,865 to 44,004
GFF1658
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -2
-1
0
1 at 40.749 kb on + strand at 40.825 kb on - strand at 40.858 kb on + strand at 40.860 kb on + strand at 41.047 kb on + strand at 41.076 kb on - strand at 41.171 kb on - strand, within GFF1657 at 41.176 kb on - strand, within GFF1657 at 41.184 kb on - strand, within GFF1657 at 41.184 kb on - strand, within GFF1657 at 41.207 kb on - strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.408 kb on + strand, within GFF1657 at 41.409 kb on - strand, within GFF1657 at 41.489 kb on - strand, within GFF1657 at 41.545 kb on + strand, within GFF1657 at 41.629 kb on + strand, within GFF1657 at 41.787 kb on + strand, within GFF1657 at 41.791 kb on + strand, within GFF1657 at 41.820 kb on + strand, within GFF1657 at 41.830 kb on + strand, within GFF1657 at 41.831 kb on - strand, within GFF1657 at 41.835 kb on - strand, within GFF1657 at 41.930 kb on + strand, within GFF1657 at 41.930 kb on + strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.931 kb on - strand, within GFF1657 at 41.963 kb on - strand, within GFF1657 at 42.117 kb on - strand, within GFF1657 at 42.117 kb on - strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.118 kb on + strand, within GFF1657 at 42.119 kb on - strand, within GFF1657 at 42.200 kb on + strand, within GFF1657 at 42.201 kb on - strand, within GFF1657 at 42.524 kb on - strand, within GFF1657 at 42.580 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.606 kb on - strand, within GFF1657 at 42.884 kb on + strand at 42.928 kb on - strand at 42.928 kb on - strand at 43.017 kb on + strand, within GFF1658 at 43.140 kb on - strand, within GFF1658 at 43.140 kb on - strand, within GFF1658 at 43.342 kb on - strand, within GFF1658 at 43.425 kb on - strand, within GFF1658 at 43.425 kb on - strand, within GFF1658 at 43.465 kb on - strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.781 kb on + strand, within GFF1658 at 43.782 kb on - strand, within GFF1658
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 40,749 + +0.1 40,825 - +0.4 40,858 + +0.7 40,860 + +0.1 41,047 + -0.2 41,076 - -0.4 41,171 - GFF1657 0.12 +1.6 41,176 - GFF1657 0.13 +0.2 41,184 - GFF1657 0.13 -2.0 41,184 - GFF1657 0.13 -0.1 41,207 - GFF1657 0.14 -0.9 41,408 + GFF1657 0.25 -0.6 41,408 + GFF1657 0.25 +0.7 41,408 + GFF1657 0.25 +0.2 41,409 - GFF1657 0.25 +0.1 41,489 - GFF1657 0.29 -0.2 41,545 + GFF1657 0.32 +0.2 41,629 + GFF1657 0.37 -0.4 41,787 + GFF1657 0.45 +0.9 41,791 + GFF1657 0.45 -0.4 41,820 + GFF1657 0.47 +0.0 41,830 + GFF1657 0.48 -0.5 41,831 - GFF1657 0.48 -0.8 41,835 - GFF1657 0.48 -0.4 41,930 + GFF1657 0.53 -0.0 41,930 + GFF1657 0.53 +0.4 41,931 - GFF1657 0.53 +0.2 41,931 - GFF1657 0.53 -1.2 41,931 - GFF1657 0.53 -0.1 41,963 - GFF1657 0.55 -0.8 42,117 - GFF1657 0.63 -0.5 42,117 - GFF1657 0.63 -0.2 42,118 + GFF1657 0.63 +0.7 42,118 + GFF1657 0.63 -0.8 42,118 + GFF1657 0.63 -1.2 42,119 - GFF1657 0.63 -0.9 42,200 + GFF1657 0.67 -0.2 42,201 - GFF1657 0.67 -0.2 42,524 - GFF1657 0.85 +0.0 42,580 - GFF1657 0.88 -0.2 42,606 - GFF1657 0.89 +0.2 42,606 - GFF1657 0.89 -0.3 42,606 - GFF1657 0.89 -0.1 42,884 + +0.6 42,928 - -0.4 42,928 - +0.6 43,017 + GFF1658 0.13 +0.2 43,140 - GFF1658 0.24 -0.1 43,140 - GFF1658 0.24 -0.1 43,342 - GFF1658 0.42 -1.6 43,425 - GFF1658 0.49 +0.3 43,425 - GFF1658 0.49 -0.4 43,465 - GFF1658 0.53 -0.7 43,781 + GFF1658 0.80 +0.1 43,781 + GFF1658 0.80 -0.7 43,781 + GFF1658 0.80 +0.5 43,782 - GFF1658 0.80 +0.2
Or see this region's nucleotide sequence