Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1074 and GFF1075 are separated by 384 nucleotides GFF1075 and GFF1076 are separated by 266 nucleotides GFF1076 and GFF1077 are separated by 635 nucleotides
GFF1074 - putative inner membrane protein, at 257,085 to 257,756
GFF1074
GFF1075 - FIG00638667: hypothetical protein, at 258,141 to 258,455
GFF1075
GFF1076 - Aerobic respiration control protein arcA, at 258,722 to 259,438
GFF1076
GFF1077 - tRNA:Cm32/Um32 methyltransferase, at 260,074 to 260,760
GFF1077
Position (kb)
258
259
260 Strain fitness (log2 ratio)
-2
-1
0
1 at 258.272 kb on + strand, within GFF1075 at 258.276 kb on - strand, within GFF1075 at 258.276 kb on - strand, within GFF1075 at 258.292 kb on - strand, within GFF1075 at 258.518 kb on - strand at 258.518 kb on - strand at 258.545 kb on + strand at 258.581 kb on + strand at 258.613 kb on - strand at 258.841 kb on + strand, within GFF1076 at 259.349 kb on + strand, within GFF1076 at 259.356 kb on + strand, within GFF1076 at 259.357 kb on - strand, within GFF1076 at 259.449 kb on + strand at 259.450 kb on - strand at 259.544 kb on + strand at 259.545 kb on - strand at 259.549 kb on - strand at 259.594 kb on - strand at 259.653 kb on - strand at 259.698 kb on + strand at 259.722 kb on + strand at 259.723 kb on - strand at 259.723 kb on - strand at 259.725 kb on - strand at 259.768 kb on + strand at 259.820 kb on + strand at 259.929 kb on + strand at 259.929 kb on + strand at 260.040 kb on - strand at 260.364 kb on - strand, within GFF1077 at 260.364 kb on - strand, within GFF1077 at 260.366 kb on + strand, within GFF1077
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 258,272 + GFF1075 0.42 -0.4 258,276 - GFF1075 0.43 +0.4 258,276 - GFF1075 0.43 -0.4 258,292 - GFF1075 0.48 -0.1 258,518 - -0.0 258,518 - +1.5 258,545 + -0.1 258,581 + +0.5 258,613 - -0.4 258,841 + GFF1076 0.17 -1.1 259,349 + GFF1076 0.87 -2.0 259,356 + GFF1076 0.88 -1.9 259,357 - GFF1076 0.89 -1.8 259,449 + -2.2 259,450 - -1.7 259,544 + -1.8 259,545 - +0.0 259,549 - +0.1 259,594 - +0.4 259,653 - +0.7 259,698 + -1.3 259,722 + -1.0 259,723 - +0.2 259,723 - +0.5 259,725 - -0.4 259,768 + -0.3 259,820 + +0.3 259,929 + +0.3 259,929 + +0.2 260,040 - +0.1 260,364 - GFF1077 0.42 +1.4 260,364 - GFF1077 0.42 -0.2 260,366 + GFF1077 0.43 +0.2
Or see this region's nucleotide sequence