Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1053 and GFF1054 are separated by 33 nucleotides GFF1054 and GFF1055 overlap by 33 nucleotides GFF1055 and GFF1056 overlap by 1 nucleotides GFF1056 and GFF1057 are separated by 83 nucleotides
GFF1053 - Lipoate-protein ligase A, at 234,765 to 235,781
GFF1053
GFF1054 - Lipoate-protein ligase A, at 235,815 to 236,459
GFF1054
GFF1055 - Phosphoserine phosphatase (EC 3.1.3.3), at 236,427 to 236,576
GFF1055
GFF1056 - Phosphoserine phosphatase (EC 3.1.3.3), at 236,576 to 237,544
GFF1056
GFF1057 - DNA repair protein RadA, at 237,628 to 239,010
GFF1057
Position (kb) 236
237
238 Strain fitness (log2 ratio) -2
-1
0
1
2 at 235.601 kb on + strand, within GFF1053 at 235.613 kb on + strand, within GFF1053 at 235.761 kb on - strand at 235.771 kb on - strand at 235.901 kb on + strand, within GFF1054 at 235.902 kb on - strand, within GFF1054 at 235.909 kb on - strand, within GFF1054 at 236.183 kb on - strand, within GFF1054 at 236.382 kb on - strand, within GFF1054 at 236.413 kb on - strand at 236.413 kb on - strand at 236.486 kb on + strand, within GFF1055 at 236.508 kb on + strand, within GFF1055 at 236.508 kb on + strand, within GFF1055 at 236.509 kb on - strand, within GFF1055 at 236.563 kb on + strand at 236.581 kb on + strand at 236.587 kb on + strand at 236.588 kb on - strand at 236.672 kb on + strand at 236.672 kb on + strand at 236.720 kb on - strand, within GFF1056 at 236.730 kb on + strand, within GFF1056 at 236.738 kb on + strand, within GFF1056 at 236.953 kb on + strand, within GFF1056 at 237.147 kb on + strand, within GFF1056 at 237.228 kb on + strand, within GFF1056 at 237.229 kb on - strand, within GFF1056 at 237.229 kb on - strand, within GFF1056 at 237.329 kb on - strand, within GFF1056 at 237.434 kb on - strand, within GFF1056 at 237.478 kb on + strand at 237.546 kb on + strand at 237.660 kb on + strand at 237.806 kb on + strand, within GFF1057 at 238.164 kb on - strand, within GFF1057 at 238.176 kb on + strand, within GFF1057
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 235,601 + GFF1053 0.82 -0.7 235,613 + GFF1053 0.83 +0.6 235,761 - -0.6 235,771 - -0.4 235,901 + GFF1054 0.13 +0.1 235,902 - GFF1054 0.13 +2.2 235,909 - GFF1054 0.15 +0.2 236,183 - GFF1054 0.57 -0.4 236,382 - GFF1054 0.88 -0.2 236,413 - +0.1 236,413 - +0.0 236,486 + GFF1055 0.39 -0.1 236,508 + GFF1055 0.54 -0.5 236,508 + GFF1055 0.54 -0.6 236,509 - GFF1055 0.55 -0.3 236,563 + -0.0 236,581 + +0.1 236,587 + +0.2 236,588 - -0.3 236,672 + +0.2 236,672 + -0.6 236,720 - GFF1056 0.15 -0.4 236,730 + GFF1056 0.16 -0.0 236,738 + GFF1056 0.17 -1.9 236,953 + GFF1056 0.39 -1.0 237,147 + GFF1056 0.59 -0.5 237,228 + GFF1056 0.67 -0.5 237,229 - GFF1056 0.67 -0.5 237,229 - GFF1056 0.67 -0.8 237,329 - GFF1056 0.78 -1.0 237,434 - GFF1056 0.89 +0.0 237,478 + -0.3 237,546 + -0.2 237,660 + +0.9 237,806 + GFF1057 0.13 -0.2 238,164 - GFF1057 0.39 -1.7 238,176 + GFF1057 0.40 +0.6
Or see this region's nucleotide sequence