Strain Fitness in Escherichia coli BW25113 around b3822

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpldA and recQ are separated by 132 nucleotidesrecQ and rhtC are separated by 63 nucleotidesrhtC and rhtB are separated by 61 nucleotides b3821: pldA - outer membrane phospholipase A (NCBI), at 4,002,885 to 4,003,754 pldA b3822: recQ - ATP-dependent DNA helicase (NCBI), at 4,003,887 to 4,005,716 recQ b3823: rhtC - threonine efflux system (NCBI), at 4,005,780 to 4,006,400 rhtC b3824: rhtB - neutral amino-acid efflux system (NCBI), at 4,006,462 to 4,007,082 rhtB Position (kb) 4003 4004 4005 4006Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4002.893 kb on - strandat 4002.893 kb on - strandat 4002.894 kb on + strandat 4002.894 kb on + strandat 4002.900 kb on - strandat 4002.908 kb on - strandat 4002.915 kb on + strandat 4002.915 kb on + strandat 4002.924 kb on - strandat 4002.924 kb on - strandat 4002.940 kb on + strandat 4002.946 kb on - strandat 4002.957 kb on + strandat 4002.981 kb on - strand, within pldAat 4002.981 kb on - strand, within pldAat 4002.984 kb on + strand, within pldAat 4003.000 kb on - strand, within pldAat 4003.000 kb on - strand, within pldAat 4003.022 kb on + strand, within pldAat 4003.022 kb on + strand, within pldAat 4003.050 kb on - strand, within pldAat 4003.076 kb on + strand, within pldAat 4003.088 kb on + strand, within pldAat 4003.250 kb on - strand, within pldAat 4003.250 kb on - strand, within pldAat 4003.259 kb on + strand, within pldAat 4003.261 kb on + strand, within pldAat 4003.348 kb on + strand, within pldAat 4003.356 kb on - strand, within pldAat 4003.359 kb on + strand, within pldAat 4003.359 kb on + strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.389 kb on + strand, within pldAat 4003.412 kb on - strand, within pldAat 4003.415 kb on + strand, within pldAat 4003.415 kb on + strand, within pldAat 4003.472 kb on + strand, within pldAat 4003.472 kb on + strand, within pldAat 4003.476 kb on + strand, within pldAat 4003.477 kb on - strand, within pldAat 4003.477 kb on - strand, within pldAat 4003.480 kb on - strand, within pldAat 4003.484 kb on - strand, within pldAat 4003.484 kb on - strand, within pldAat 4003.489 kb on - strand, within pldAat 4003.512 kb on + strand, within pldAat 4003.512 kb on + strand, within pldAat 4003.586 kb on - strand, within pldAat 4003.614 kb on + strand, within pldAat 4003.614 kb on + strand, within pldAat 4003.616 kb on + strand, within pldAat 4003.620 kb on + strand, within pldAat 4003.632 kb on - strand, within pldAat 4003.632 kb on - strand, within pldAat 4003.660 kb on - strand, within pldAat 4003.723 kb on + strandat 4003.731 kb on - strandat 4003.735 kb on + strandat 4003.742 kb on - strandat 4003.993 kb on + strandat 4003.993 kb on + strandat 4003.995 kb on + strandat 4003.995 kb on + strandat 4004.003 kb on - strandat 4004.003 kb on - strandat 4004.028 kb on - strandat 4004.083 kb on + strand, within recQat 4004.133 kb on + strand, within recQat 4004.197 kb on + strand, within recQat 4004.374 kb on - strand, within recQat 4004.393 kb on - strand, within recQat 4004.436 kb on + strand, within recQat 4004.478 kb on - strand, within recQat 4004.535 kb on - strand, within recQat 4004.568 kb on - strandat 4004.573 kb on - strand, within recQat 4004.586 kb on - strand, within recQat 4004.586 kb on - strand, within recQat 4004.590 kb on + strand, within recQat 4004.632 kb on - strand, within recQat 4004.632 kb on - strand, within recQat 4004.651 kb on + strand, within recQat 4004.673 kb on - strand, within recQat 4004.740 kb on + strand, within recQat 4004.740 kb on + strand, within recQat 4004.816 kb on + strand, within recQat 4004.816 kb on + strand, within recQat 4004.818 kb on + strand, within recQat 4004.868 kb on + strand, within recQat 4004.868 kb on + strand, within recQat 4004.884 kb on + strand, within recQat 4004.885 kb on + strand, within recQat 4004.908 kb on - strand, within recQat 4004.924 kb on - strand, within recQat 4004.924 kb on - strand, within recQat 4004.938 kb on + strand, within recQat 4004.970 kb on - strand, within recQat 4004.981 kb on + strand, within recQat 4004.981 kb on + strand, within recQat 4005.039 kb on - strand, within recQat 4005.064 kb on + strand, within recQat 4005.072 kb on - strand, within recQat 4005.103 kb on + strand, within recQat 4005.119 kb on - strand, within recQat 4005.129 kb on - strand, within recQat 4005.138 kb on - strand, within recQat 4005.146 kb on + strand, within recQat 4005.192 kb on - strand, within recQat 4005.198 kb on + strand, within recQat 4005.219 kb on - strand, within recQat 4005.219 kb on - strand, within recQat 4005.226 kb on + strand, within recQat 4005.226 kb on + strand, within recQat 4005.237 kb on + strand, within recQat 4005.245 kb on + strand, within recQat 4005.245 kb on + strand, within recQat 4005.366 kb on - strand, within recQat 4005.387 kb on + strand, within recQat 4005.389 kb on + strand, within recQat 4005.389 kb on + strand, within recQat 4005.481 kb on + strand, within recQat 4005.555 kb on + strandat 4005.556 kb on - strandat 4005.557 kb on + strandat 4005.573 kb on - strandat 4005.573 kb on - strandat 4005.618 kb on - strandat 4005.622 kb on + strandat 4005.622 kb on + strandat 4005.631 kb on + strandat 4005.664 kb on - strandat 4005.675 kb on + strandat 4005.675 kb on + strandat 4005.817 kb on + strandat 4005.865 kb on - strand, within rhtCat 4005.997 kb on - strand, within rhtCat 4005.998 kb on + strand, within rhtCat 4006.017 kb on + strand, within rhtCat 4006.051 kb on + strand, within rhtCat 4006.103 kb on + strand, within rhtCat 4006.208 kb on + strand, within rhtCat 4006.244 kb on + strand, within rhtCat 4006.244 kb on + strand, within rhtCat 4006.275 kb on - strand, within rhtCat 4006.304 kb on + strand, within rhtCat 4006.323 kb on - strand, within rhtCat 4006.331 kb on + strand, within rhtCat 4006.420 kb on - strandat 4006.421 kb on - strandat 4006.443 kb on - strandat 4006.480 kb on + strandat 4006.480 kb on + strandat 4006.486 kb on - strandat 4006.489 kb on - strandat 4006.491 kb on + strandat 4006.501 kb on + strandat 4006.501 kb on - strandat 4006.501 kb on - strandat 4006.528 kb on - strand, within rhtBat 4006.542 kb on + strand, within rhtBat 4006.545 kb on - strand, within rhtBat 4006.558 kb on + strand, within rhtBat 4006.566 kb on - strand, within rhtBat 4006.602 kb on + strand, within rhtBat 4006.602 kb on + strand, within rhtBat 4006.610 kb on - strand, within rhtBat 4006.622 kb on - strand, within rhtBat 4006.622 kb on - strand, within rhtBat 4006.627 kb on - strand, within rhtBat 4006.627 kb on - strand, within rhtBat 4006.638 kb on + strand, within rhtBat 4006.638 kb on + strand, within rhtBat 4006.640 kb on + strand, within rhtBat 4006.645 kb on - strand, within rhtBat 4006.645 kb on - strand, within rhtBat 4006.646 kb on - strand, within rhtB

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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4,002,893 - +0.1
4,002,893 - +0.1
4,002,894 + -0.0
4,002,894 + -0.2
4,002,900 - -0.6
4,002,908 - -1.8
4,002,915 + -0.8
4,002,915 + -1.1
4,002,924 - +1.0
4,002,924 - -0.8
4,002,940 + +2.2
4,002,946 - +1.2
4,002,957 + -0.9
4,002,981 - pldA b3821 0.11 +0.0
4,002,981 - pldA b3821 0.11 -1.5
4,002,984 + pldA b3821 0.11 +0.5
4,003,000 - pldA b3821 0.13 -1.5
4,003,000 - pldA b3821 0.13 -1.5
4,003,022 + pldA b3821 0.16 -0.7
4,003,022 + pldA b3821 0.16 +0.4
4,003,050 - pldA b3821 0.19 -0.2
4,003,076 + pldA b3821 0.22 -0.3
4,003,088 + pldA b3821 0.23 +0.7
4,003,250 - pldA b3821 0.42 +0.1
4,003,250 - pldA b3821 0.42 -0.4
4,003,259 + pldA b3821 0.43 -0.8
4,003,261 + pldA b3821 0.43 +0.1
4,003,348 + pldA b3821 0.53 +1.2
4,003,356 - pldA b3821 0.54 -0.6
4,003,359 + pldA b3821 0.54 -1.3
4,003,359 + pldA b3821 0.54 -2.7
4,003,367 - pldA b3821 0.55 +0.4
4,003,367 - pldA b3821 0.55 +1.0
4,003,367 - pldA b3821 0.55 +0.0
4,003,367 - pldA b3821 0.55 -0.7
4,003,389 + pldA b3821 0.58 -0.1
4,003,412 - pldA b3821 0.61 -1.9
4,003,415 + pldA b3821 0.61 -0.1
4,003,415 + pldA b3821 0.61 +0.5
4,003,472 + pldA b3821 0.67 -0.7
4,003,472 + pldA b3821 0.67 -0.7
4,003,476 + pldA b3821 0.68 -0.2
4,003,477 - pldA b3821 0.68 +0.0
4,003,477 - pldA b3821 0.68 +0.2
4,003,480 - pldA b3821 0.68 +0.5
4,003,484 - pldA b3821 0.69 -0.3
4,003,484 - pldA b3821 0.69 +0.2
4,003,489 - pldA b3821 0.69 +1.2
4,003,512 + pldA b3821 0.72 +0.0
4,003,512 + pldA b3821 0.72 +0.5
4,003,586 - pldA b3821 0.81 -0.3
4,003,614 + pldA b3821 0.84 -1.9
4,003,614 + pldA b3821 0.84 -1.4
4,003,616 + pldA b3821 0.84 -0.4
4,003,620 + pldA b3821 0.84 -0.4
4,003,632 - pldA b3821 0.86 +0.2
4,003,632 - pldA b3821 0.86 -1.1
4,003,660 - pldA b3821 0.89 +0.6
4,003,723 + -0.9
4,003,731 - -1.0
4,003,735 + -0.9
4,003,742 - -0.4
4,003,993 + +0.6
4,003,993 + +0.6
4,003,995 + +0.4
4,003,995 + -0.4
4,004,003 - +3.2
4,004,003 - +0.2
4,004,028 - -2.4
4,004,083 + recQ b3822 0.11 +1.9
4,004,133 + recQ b3822 0.13 -0.4
4,004,197 + recQ b3822 0.17 -1.4
4,004,374 - recQ b3822 0.27 -1.2
4,004,393 - recQ b3822 0.28 -0.8
4,004,436 + recQ b3822 0.30 +0.2
4,004,478 - recQ b3822 0.32 -0.6
4,004,535 - recQ b3822 0.35 -0.2
4,004,568 - +0.2
4,004,573 - recQ b3822 0.37 -0.4
4,004,586 - recQ b3822 0.38 -0.1
4,004,586 - recQ b3822 0.38 +0.0
4,004,590 + recQ b3822 0.38 -0.8
4,004,632 - recQ b3822 0.41 +1.2
4,004,632 - recQ b3822 0.41 -1.8
4,004,651 + recQ b3822 0.42 -1.1
4,004,673 - recQ b3822 0.43 -0.4
4,004,740 + recQ b3822 0.47 -0.2
4,004,740 + recQ b3822 0.47 +0.3
4,004,816 + recQ b3822 0.51 -1.4
4,004,816 + recQ b3822 0.51 +0.2
4,004,818 + recQ b3822 0.51 +0.8
4,004,868 + recQ b3822 0.54 +0.5
4,004,868 + recQ b3822 0.54 +0.4
4,004,884 + recQ b3822 0.54 -0.6
4,004,885 + recQ b3822 0.55 +0.4
4,004,908 - recQ b3822 0.56 +0.4
4,004,924 - recQ b3822 0.57 -1.1
4,004,924 - recQ b3822 0.57 -0.7
4,004,938 + recQ b3822 0.57 +1.6
4,004,970 - recQ b3822 0.59 +0.0
4,004,981 + recQ b3822 0.60 +0.8
4,004,981 + recQ b3822 0.60 -0.0
4,005,039 - recQ b3822 0.63 -0.6
4,005,064 + recQ b3822 0.64 -1.0
4,005,072 - recQ b3822 0.65 +0.8
4,005,103 + recQ b3822 0.66 +0.1
4,005,119 - recQ b3822 0.67 -0.1
4,005,129 - recQ b3822 0.68 -0.1
4,005,138 - recQ b3822 0.68 -1.1
4,005,146 + recQ b3822 0.69 -1.1
4,005,192 - recQ b3822 0.71 -0.1
4,005,198 + recQ b3822 0.72 -0.8
4,005,219 - recQ b3822 0.73 +0.9
4,005,219 - recQ b3822 0.73 -0.4
4,005,226 + recQ b3822 0.73 +0.2
4,005,226 + recQ b3822 0.73 -0.7
4,005,237 + recQ b3822 0.74 -1.4
4,005,245 + recQ b3822 0.74 +0.5
4,005,245 + recQ b3822 0.74 -0.4
4,005,366 - recQ b3822 0.81 -0.4
4,005,387 + recQ b3822 0.82 +0.4
4,005,389 + recQ b3822 0.82 -0.8
4,005,389 + recQ b3822 0.82 -2.0
4,005,481 + recQ b3822 0.87 +0.6
4,005,555 + -0.0
4,005,556 - -0.7
4,005,557 + +0.5
4,005,573 - +0.2
4,005,573 - -0.8
4,005,618 - +0.2
4,005,622 + +1.6
4,005,622 + +0.4
4,005,631 + -0.2
4,005,664 - +0.5
4,005,675 + -0.3
4,005,675 + +0.6
4,005,817 + +1.5
4,005,865 - rhtC b3823 0.14 +0.6
4,005,997 - rhtC b3823 0.35 +0.5
4,005,998 + rhtC b3823 0.35 -0.6
4,006,017 + rhtC b3823 0.38 -0.4
4,006,051 + rhtC b3823 0.44 -1.8
4,006,103 + rhtC b3823 0.52 +0.5
4,006,208 + rhtC b3823 0.69 -1.1
4,006,244 + rhtC b3823 0.75 +0.2
4,006,244 + rhtC b3823 0.75 -0.6
4,006,275 - rhtC b3823 0.80 -0.1
4,006,304 + rhtC b3823 0.84 -0.3
4,006,323 - rhtC b3823 0.87 +0.4
4,006,331 + rhtC b3823 0.89 -0.1
4,006,420 - -1.6
4,006,421 - -0.9
4,006,443 - +1.2
4,006,480 + +1.2
4,006,480 + +0.1
4,006,486 - -1.8
4,006,489 - -0.6
4,006,491 + +0.2
4,006,501 + +0.0
4,006,501 - +0.0
4,006,501 - +0.6
4,006,528 - rhtB b3824 0.11 +0.1
4,006,542 + rhtB b3824 0.13 +0.2
4,006,545 - rhtB b3824 0.13 +0.4
4,006,558 + rhtB b3824 0.15 -2.7
4,006,566 - rhtB b3824 0.17 -0.6
4,006,602 + rhtB b3824 0.23 +0.1
4,006,602 + rhtB b3824 0.23 -0.4
4,006,610 - rhtB b3824 0.24 +0.2
4,006,622 - rhtB b3824 0.26 +0.2
4,006,622 - rhtB b3824 0.26 +0.5
4,006,627 - rhtB b3824 0.27 -0.4
4,006,627 - rhtB b3824 0.27 +0.2
4,006,638 + rhtB b3824 0.28 -0.4
4,006,638 + rhtB b3824 0.28 -1.5
4,006,640 + rhtB b3824 0.29 -0.3
4,006,645 - rhtB b3824 0.29 +0.3
4,006,645 - rhtB b3824 0.29 +0.4
4,006,646 - rhtB b3824 0.30 -0.6

Or see this region's nucleotide sequence