Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsF and priB are separated by 6 nucleotides priB and rpsR are separated by 4 nucleotides rpsR and rplI are separated by 41 nucleotides rplI and yjfZ are separated by 70 nucleotides
b4200: rpsF - 30S ribosomal protein S6 (NCBI), at 4,423,141 to 4,423,536
rpsF
b4201: priB - primosomal replication protein N (NCBI), at 4,423,543 to 4,423,857
priB
b4202: rpsR - 30S ribosomal protein S18 (NCBI), at 4,423,862 to 4,424,089
rpsR
b4203: rplI - 50S ribosomal protein L9 (NCBI), at 4,424,131 to 4,424,580
rplI
b4204: yjfZ - hypothetical protein (NCBI), at 4,424,651 to 4,425,445
yjfZ
Position (kb)
4423
4424
4425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4423.415 kb on + strand, within rpsF at 4423.552 kb on + strand at 4423.552 kb on + strand at 4423.563 kb on + strand at 4423.605 kb on + strand, within priB at 4423.636 kb on + strand, within priB at 4423.736 kb on + strand, within priB at 4423.752 kb on + strand, within priB at 4423.815 kb on + strand, within priB at 4424.063 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.065 kb on + strand, within rpsR at 4424.099 kb on + strand at 4424.214 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.229 kb on + strand, within rplI at 4424.248 kb on + strand, within rplI at 4424.360 kb on + strand, within rplI at 4424.427 kb on + strand, within rplI at 4424.501 kb on + strand, within rplI at 4424.510 kb on + strand, within rplI at 4424.523 kb on + strand, within rplI at 4424.525 kb on + strand, within rplI at 4424.592 kb on + strand at 4424.615 kb on + strand at 4424.669 kb on + strand at 4424.676 kb on - strand at 4424.677 kb on - strand at 4424.678 kb on + strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.684 kb on - strand at 4424.690 kb on + strand at 4424.690 kb on + strand at 4424.705 kb on + strand at 4424.708 kb on - strand at 4424.722 kb on - strand at 4424.723 kb on + strand at 4424.727 kb on - strand at 4424.727 kb on - strand at 4424.728 kb on + strand at 4424.728 kb on + strand at 4424.728 kb on - strand at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.733 kb on + strand, within yjfZ at 4424.744 kb on - strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.747 kb on + strand, within yjfZ at 4424.749 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.755 kb on - strand, within yjfZ at 4424.844 kb on - strand, within yjfZ at 4424.844 kb on - strand, within yjfZ at 4424.855 kb on + strand, within yjfZ at 4424.878 kb on + strand, within yjfZ at 4424.881 kb on + strand, within yjfZ at 4424.892 kb on - strand, within yjfZ at 4424.898 kb on - strand, within yjfZ at 4424.898 kb on - strand, within yjfZ at 4424.906 kb on + strand, within yjfZ at 4424.906 kb on + strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.909 kb on - strand, within yjfZ at 4424.915 kb on + strand, within yjfZ at 4424.915 kb on + strand, within yjfZ at 4424.916 kb on + strand, within yjfZ at 4424.916 kb on + strand, within yjfZ at 4424.946 kb on - strand, within yjfZ at 4424.960 kb on - strand, within yjfZ at 4424.960 kb on - strand, within yjfZ at 4424.972 kb on + strand, within yjfZ at 4424.975 kb on + strand, within yjfZ at 4424.976 kb on + strand, within yjfZ at 4424.981 kb on + strand, within yjfZ at 4424.983 kb on - strand, within yjfZ at 4424.984 kb on - strand, within yjfZ at 4424.989 kb on - strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.003 kb on + strand, within yjfZ at 4425.005 kb on + strand, within yjfZ at 4425.008 kb on + strand, within yjfZ at 4425.025 kb on - strand, within yjfZ at 4425.060 kb on + strand, within yjfZ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 4,423,415 + rpsF b4200 0.69 -2.4 4,423,552 + +0.0 4,423,552 + +0.0 4,423,563 + -2.8 4,423,605 + priB b4201 0.20 -1.5 4,423,636 + priB b4201 0.30 -1.0 4,423,736 + priB b4201 0.61 +0.0 4,423,752 + priB b4201 0.66 -1.0 4,423,815 + priB b4201 0.86 -1.0 4,424,063 + rpsR b4202 0.88 +0.4 4,424,065 + rpsR b4202 0.89 +0.0 4,424,065 + rpsR b4202 0.89 -1.3 4,424,065 + rpsR b4202 0.89 -1.0 4,424,065 + rpsR b4202 0.89 -0.2 4,424,065 + rpsR b4202 0.89 -0.8 4,424,065 + rpsR b4202 0.89 +0.0 4,424,099 + +0.6 4,424,214 + rplI b4203 0.18 +0.7 4,424,229 + rplI b4203 0.22 +0.4 4,424,229 + rplI b4203 0.22 +1.2 4,424,229 + rplI b4203 0.22 +1.4 4,424,229 + rplI b4203 0.22 +0.4 4,424,248 + rplI b4203 0.26 +1.0 4,424,360 + rplI b4203 0.51 +0.0 4,424,427 + rplI b4203 0.66 -0.7 4,424,501 + rplI b4203 0.82 -0.6 4,424,510 + rplI b4203 0.84 +1.0 4,424,523 + rplI b4203 0.87 +0.9 4,424,525 + rplI b4203 0.88 +1.8 4,424,592 + -1.8 4,424,615 + -0.6 4,424,669 + +1.0 4,424,676 - +1.5 4,424,677 - -0.5 4,424,678 + -0.7 4,424,684 - -2.0 4,424,684 - -0.2 4,424,684 - -0.2 4,424,684 - +0.4 4,424,690 + -0.6 4,424,690 + +0.0 4,424,705 + +0.1 4,424,708 - -1.8 4,424,722 - +1.3 4,424,723 + -0.7 4,424,727 - +0.0 4,424,727 - -1.3 4,424,728 + +1.0 4,424,728 + -0.8 4,424,728 - -0.4 4,424,733 + yjfZ b4204 0.10 -2.4 4,424,733 + yjfZ b4204 0.10 -0.4 4,424,733 + yjfZ b4204 0.10 +1.4 4,424,733 + yjfZ b4204 0.10 +3.0 4,424,733 + yjfZ b4204 0.10 +0.1 4,424,744 - yjfZ b4204 0.12 +0.1 4,424,747 + yjfZ b4204 0.12 -0.6 4,424,747 + yjfZ b4204 0.12 +0.4 4,424,747 + yjfZ b4204 0.12 +1.4 4,424,749 - yjfZ b4204 0.12 +0.0 4,424,755 - yjfZ b4204 0.13 +0.8 4,424,755 - yjfZ b4204 0.13 -1.8 4,424,755 - yjfZ b4204 0.13 +0.0 4,424,755 - yjfZ b4204 0.13 +0.8 4,424,755 - yjfZ b4204 0.13 +1.6 4,424,844 - yjfZ b4204 0.24 -0.5 4,424,844 - yjfZ b4204 0.24 -1.2 4,424,855 + yjfZ b4204 0.26 +0.7 4,424,878 + yjfZ b4204 0.29 -0.7 4,424,881 + yjfZ b4204 0.29 +0.1 4,424,892 - yjfZ b4204 0.30 +0.4 4,424,898 - yjfZ b4204 0.31 -1.6 4,424,898 - yjfZ b4204 0.31 -0.8 4,424,906 + yjfZ b4204 0.32 +0.7 4,424,906 + yjfZ b4204 0.32 -0.4 4,424,909 - yjfZ b4204 0.32 -1.2 4,424,909 - yjfZ b4204 0.32 -1.2 4,424,909 - yjfZ b4204 0.32 -0.2 4,424,909 - yjfZ b4204 0.32 +0.5 4,424,915 + yjfZ b4204 0.33 +0.2 4,424,915 + yjfZ b4204 0.33 -1.2 4,424,916 + yjfZ b4204 0.33 +2.2 4,424,916 + yjfZ b4204 0.33 +0.5 4,424,946 - yjfZ b4204 0.37 -0.1 4,424,960 - yjfZ b4204 0.39 +0.4 4,424,960 - yjfZ b4204 0.39 -0.3 4,424,972 + yjfZ b4204 0.40 -0.3 4,424,975 + yjfZ b4204 0.41 +0.3 4,424,976 + yjfZ b4204 0.41 +1.3 4,424,981 + yjfZ b4204 0.42 -1.3 4,424,983 - yjfZ b4204 0.42 -1.6 4,424,984 - yjfZ b4204 0.42 -0.9 4,424,989 - yjfZ b4204 0.43 +0.1 4,425,003 + yjfZ b4204 0.44 -0.1 4,425,003 + yjfZ b4204 0.44 -1.8 4,425,003 + yjfZ b4204 0.44 +1.0 4,425,003 + yjfZ b4204 0.44 -0.5 4,425,003 + yjfZ b4204 0.44 +0.7 4,425,003 + yjfZ b4204 0.44 +0.7 4,425,003 + yjfZ b4204 0.44 +0.2 4,425,003 + yjfZ b4204 0.44 +0.9 4,425,003 + yjfZ b4204 0.44 +0.7 4,425,005 + yjfZ b4204 0.45 +1.0 4,425,008 + yjfZ b4204 0.45 +1.2 4,425,025 - yjfZ b4204 0.47 -0.6 4,425,060 + yjfZ b4204 0.51 +0.1
Or see this region's nucleotide sequence