Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galP and sprT are separated by 76 nucleotides sprT and endA are separated by 94 nucleotides endA and yggJ are separated by 79 nucleotides yggJ and gshB are separated by 12 nucleotides
b2943: galP - galactose:H+ symporter (galP) (from data) , at 3,086,306 to 3,087,700
galP
b2944: sprT - hypothetical protein (NCBI), at 3,087,777 to 3,088,274
sprT
b2945: endA - DNA-specific endonuclease I (NCBI), at 3,088,369 to 3,089,076
endA
b2946: yggJ - orf, hypothetical protein (VIMSS), at 3,089,156 to 3,089,887
yggJ
b2947: gshB - glutathione synthetase (NCBI), at 3,089,900 to 3,090,850
gshB
Position (kb)
3088
3089
3090 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3087.376 kb on - strand, within galP at 3087.414 kb on + strand, within galP at 3087.451 kb on - strand, within galP at 3087.451 kb on - strand, within galP at 3087.517 kb on + strand, within galP at 3087.517 kb on + strand, within galP at 3087.523 kb on - strand, within galP at 3087.525 kb on - strand, within galP at 3087.537 kb on - strand, within galP at 3087.572 kb on + strand at 3087.600 kb on + strand at 3087.704 kb on + strand at 3087.739 kb on - strand at 3087.759 kb on + strand at 3087.759 kb on + strand at 3087.802 kb on - strand at 3087.872 kb on - strand, within sprT at 3087.872 kb on - strand, within sprT at 3087.877 kb on - strand, within sprT at 3087.890 kb on + strand, within sprT at 3087.892 kb on + strand, within sprT at 3087.892 kb on + strand, within sprT at 3087.898 kb on - strand, within sprT at 3087.898 kb on - strand, within sprT at 3087.928 kb on + strand, within sprT at 3087.928 kb on + strand, within sprT at 3087.950 kb on + strand, within sprT at 3087.999 kb on + strand, within sprT at 3088.001 kb on - strand at 3088.001 kb on - strand, within sprT at 3088.048 kb on + strand, within sprT at 3088.048 kb on + strand, within sprT at 3088.099 kb on + strand, within sprT at 3088.350 kb on + strand at 3088.350 kb on + strand at 3088.406 kb on - strand at 3088.406 kb on - strand at 3088.453 kb on + strand, within endA at 3088.619 kb on - strand, within endA at 3088.683 kb on - strand, within endA at 3088.724 kb on + strand, within endA at 3088.732 kb on - strand, within endA at 3088.732 kb on - strand, within endA at 3088.875 kb on - strand, within endA at 3088.885 kb on - strand, within endA at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.962 kb on + strand, within endA at 3088.970 kb on - strand, within endA at 3088.970 kb on - strand, within endA at 3089.020 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.038 kb on - strand at 3089.191 kb on + strand at 3089.281 kb on - strand, within yggJ at 3089.295 kb on - strand, within yggJ at 3089.411 kb on + strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.476 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.586 kb on - strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.634 kb on + strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.642 kb on - strand, within yggJ at 3089.895 kb on + strand at 3089.924 kb on - strand at 3090.004 kb on + strand, within gshB
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 3,087,376 - galP b2943 0.77 +2.3 3,087,414 + galP b2943 0.79 +1.0 3,087,451 - galP b2943 0.82 -0.1 3,087,451 - galP b2943 0.82 -1.4 3,087,517 + galP b2943 0.87 -0.4 3,087,517 + galP b2943 0.87 +1.9 3,087,523 - galP b2943 0.87 +0.3 3,087,525 - galP b2943 0.87 +0.2 3,087,537 - galP b2943 0.88 -1.1 3,087,572 + -0.1 3,087,600 + -0.6 3,087,704 + +0.9 3,087,739 - +0.0 3,087,759 + -0.3 3,087,759 + +2.5 3,087,802 - +0.8 3,087,872 - sprT b2944 0.19 +0.2 3,087,872 - sprT b2944 0.19 -0.1 3,087,877 - sprT b2944 0.20 -0.3 3,087,890 + sprT b2944 0.23 -0.1 3,087,892 + sprT b2944 0.23 +0.9 3,087,892 + sprT b2944 0.23 +0.2 3,087,898 - sprT b2944 0.24 -0.4 3,087,898 - sprT b2944 0.24 -0.2 3,087,928 + sprT b2944 0.30 +0.1 3,087,928 + sprT b2944 0.30 +0.2 3,087,950 + sprT b2944 0.35 +1.0 3,087,999 + sprT b2944 0.45 +0.7 3,088,001 - +0.3 3,088,001 - sprT b2944 0.45 +0.4 3,088,048 + sprT b2944 0.54 -2.0 3,088,048 + sprT b2944 0.54 +0.9 3,088,099 + sprT b2944 0.65 +1.1 3,088,350 + +0.9 3,088,350 + +0.9 3,088,406 - -1.7 3,088,406 - -1.1 3,088,453 + endA b2945 0.12 -1.0 3,088,619 - endA b2945 0.35 +0.9 3,088,683 - endA b2945 0.44 -1.5 3,088,724 + endA b2945 0.50 -2.2 3,088,732 - endA b2945 0.51 +0.3 3,088,732 - endA b2945 0.51 -2.6 3,088,875 - endA b2945 0.71 -1.4 3,088,885 - endA b2945 0.73 -1.4 3,088,962 + endA b2945 0.84 -1.7 3,088,962 + endA b2945 0.84 -0.3 3,088,962 + endA b2945 0.84 -0.1 3,088,970 - endA b2945 0.85 -1.1 3,088,970 - endA b2945 0.85 -1.7 3,089,020 - -0.7 3,089,038 - +0.7 3,089,038 - -0.4 3,089,038 - +1.9 3,089,191 + -1.1 3,089,281 - yggJ b2946 0.17 -0.1 3,089,295 - yggJ b2946 0.19 -0.3 3,089,411 + yggJ b2946 0.35 -2.5 3,089,476 - yggJ b2946 0.44 -0.5 3,089,476 - yggJ b2946 0.44 -1.0 3,089,586 - yggJ b2946 0.59 -1.9 3,089,586 - yggJ b2946 0.59 -1.2 3,089,634 + yggJ b2946 0.65 -1.5 3,089,634 + yggJ b2946 0.65 -1.4 3,089,642 - yggJ b2946 0.66 +0.5 3,089,642 - yggJ b2946 0.66 +1.9 3,089,895 + -1.5 3,089,924 - +0.8 3,090,004 + gshB b2947 0.11 +1.0
Or see this region's nucleotide sequence