Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehS and yehT are separated by 46 nucleotides yehT and yehU overlap by 4 nucleotides yehU and mlrA are separated by 221 nucleotides
b2124: yehS - hypothetical protein (NCBI), at 2,209,748 to 2,210,218
yehS
b2125: yehT - orf, hypothetical protein (VIMSS), at 2,210,265 to 2,210,984
yehT
b2126: yehU - predicted sensory kinase in two-component system with YehT (NCBI), at 2,210,981 to 2,212,666
yehU
b2127: mlrA - DNA-binding transcriptional regulator (NCBI), at 2,212,888 to 2,213,619
mlrA
Position (kb)
2210
2211
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2210.041 kb on - strand, within yehS at 2210.106 kb on - strand, within yehS at 2210.106 kb on - strand, within yehS at 2210.138 kb on - strand, within yehS at 2210.161 kb on + strand, within yehS at 2210.185 kb on + strand at 2210.193 kb on - strand at 2210.203 kb on - strand at 2210.313 kb on - strand at 2210.408 kb on - strand, within yehT at 2210.411 kb on - strand, within yehT at 2210.492 kb on + strand, within yehT at 2210.492 kb on + strand, within yehT at 2210.498 kb on - strand, within yehT at 2210.502 kb on + strand, within yehT at 2210.695 kb on - strand, within yehT at 2210.695 kb on - strand, within yehT at 2210.715 kb on + strand, within yehT at 2210.715 kb on + strand, within yehT at 2210.858 kb on - strand, within yehT at 2210.858 kb on - strand, within yehT at 2210.936 kb on + strand at 2210.954 kb on - strand at 2210.962 kb on - strand at 2210.962 kb on - strand at 2211.049 kb on - strand at 2211.051 kb on + strand at 2211.130 kb on - strand at 2211.130 kb on - strand at 2211.207 kb on - strand, within yehU at 2211.207 kb on - strand, within yehU at 2211.218 kb on - strand, within yehU at 2211.273 kb on - strand, within yehU at 2211.273 kb on - strand, within yehU at 2211.371 kb on - strand, within yehU at 2211.399 kb on - strand, within yehU at 2211.451 kb on - strand, within yehU at 2211.464 kb on - strand, within yehU at 2211.539 kb on - strand, within yehU at 2211.539 kb on - strand, within yehU at 2211.651 kb on + strand, within yehU at 2211.675 kb on + strand, within yehU at 2211.688 kb on - strand, within yehU at 2211.688 kb on - strand, within yehU at 2211.782 kb on - strand, within yehU at 2211.806 kb on - strand, within yehU at 2211.826 kb on + strand, within yehU at 2211.834 kb on - strand, within yehU at 2211.961 kb on - strand, within yehU at 2211.972 kb on - strand, within yehU at 2211.972 kb on - strand, within yehU at 2212.028 kb on + strand, within yehU at 2212.168 kb on + strand, within yehU at 2212.177 kb on - strand, within yehU at 2212.177 kb on - strand, within yehU at 2212.256 kb on - strand, within yehU at 2212.264 kb on - strand, within yehU at 2212.294 kb on + strand, within yehU at 2212.358 kb on - strand, within yehU at 2212.358 kb on - strand, within yehU at 2212.365 kb on + strand, within yehU at 2212.373 kb on - strand, within yehU at 2212.438 kb on - strand, within yehU at 2212.438 kb on - strand, within yehU at 2212.564 kb on + strand at 2213.000 kb on + strand, within mlrA at 2213.008 kb on - strand, within mlrA at 2213.008 kb on - strand, within mlrA at 2213.066 kb on - strand, within mlrA at 2213.222 kb on - strand, within mlrA at 2213.244 kb on + strand, within mlrA at 2213.370 kb on + strand, within mlrA at 2213.370 kb on + strand, within mlrA at 2213.444 kb on + strand, within mlrA at 2213.484 kb on + strand, within mlrA at 2213.506 kb on + strand, within mlrA at 2213.506 kb on + strand, within mlrA at 2213.513 kb on + strand, within mlrA at 2213.641 kb on + strand at 2213.641 kb on + strand at 2213.646 kb on + strand at 2213.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 2,210,041 - yehS b2124 0.62 +0.7 2,210,106 - yehS b2124 0.76 -0.2 2,210,106 - yehS b2124 0.76 +1.2 2,210,138 - yehS b2124 0.83 +0.3 2,210,161 + yehS b2124 0.88 +0.9 2,210,185 + +0.0 2,210,193 - +0.2 2,210,203 - +0.6 2,210,313 - +0.6 2,210,408 - yehT b2125 0.20 +0.3 2,210,411 - yehT b2125 0.20 +0.1 2,210,492 + yehT b2125 0.32 +0.6 2,210,492 + yehT b2125 0.32 +0.4 2,210,498 - yehT b2125 0.32 +0.9 2,210,502 + yehT b2125 0.33 -0.9 2,210,695 - yehT b2125 0.60 -0.4 2,210,695 - yehT b2125 0.60 +0.3 2,210,715 + yehT b2125 0.62 +2.2 2,210,715 + yehT b2125 0.62 -0.6 2,210,858 - yehT b2125 0.82 -1.5 2,210,858 - yehT b2125 0.82 +0.9 2,210,936 + +0.0 2,210,954 - -0.4 2,210,962 - +1.1 2,210,962 - +0.2 2,211,049 - +0.6 2,211,051 + -0.3 2,211,130 - +3.2 2,211,130 - +0.1 2,211,207 - yehU b2126 0.13 -1.0 2,211,207 - yehU b2126 0.13 +0.0 2,211,218 - yehU b2126 0.14 -0.3 2,211,273 - yehU b2126 0.17 -0.4 2,211,273 - yehU b2126 0.17 -0.2 2,211,371 - yehU b2126 0.23 +0.3 2,211,399 - yehU b2126 0.25 -0.3 2,211,451 - yehU b2126 0.28 -0.4 2,211,464 - yehU b2126 0.29 -0.6 2,211,539 - yehU b2126 0.33 -0.4 2,211,539 - yehU b2126 0.33 -0.4 2,211,651 + yehU b2126 0.40 -0.6 2,211,675 + yehU b2126 0.41 -0.6 2,211,688 - yehU b2126 0.42 -0.3 2,211,688 - yehU b2126 0.42 +0.1 2,211,782 - yehU b2126 0.48 -0.0 2,211,806 - yehU b2126 0.49 -1.2 2,211,826 + yehU b2126 0.50 +1.5 2,211,834 - yehU b2126 0.51 -1.5 2,211,961 - yehU b2126 0.58 -1.0 2,211,972 - yehU b2126 0.59 +0.9 2,211,972 - yehU b2126 0.59 -1.0 2,212,028 + yehU b2126 0.62 +0.6 2,212,168 + yehU b2126 0.70 -2.0 2,212,177 - yehU b2126 0.71 +0.3 2,212,177 - yehU b2126 0.71 +1.1 2,212,256 - yehU b2126 0.76 -0.3 2,212,264 - yehU b2126 0.76 -1.0 2,212,294 + yehU b2126 0.78 -0.1 2,212,358 - yehU b2126 0.82 -0.7 2,212,358 - yehU b2126 0.82 -1.9 2,212,365 + yehU b2126 0.82 -1.3 2,212,373 - yehU b2126 0.83 -1.4 2,212,438 - yehU b2126 0.86 -0.4 2,212,438 - yehU b2126 0.86 -0.9 2,212,564 + +1.0 2,213,000 + mlrA b2127 0.15 -0.4 2,213,008 - mlrA b2127 0.16 -0.2 2,213,008 - mlrA b2127 0.16 +0.0 2,213,066 - mlrA b2127 0.24 +1.3 2,213,222 - mlrA b2127 0.46 -1.0 2,213,244 + mlrA b2127 0.49 +0.1 2,213,370 + mlrA b2127 0.66 +1.2 2,213,370 + mlrA b2127 0.66 -0.2 2,213,444 + mlrA b2127 0.76 -1.2 2,213,484 + mlrA b2127 0.81 -0.1 2,213,506 + mlrA b2127 0.84 +0.1 2,213,506 + mlrA b2127 0.84 +1.6 2,213,513 + mlrA b2127 0.85 +1.3 2,213,641 + +0.6 2,213,641 + +1.4 2,213,646 + +0.3 2,213,654 - -0.7
Or see this region's nucleotide sequence