Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tap and tar are separated by 45 nucleotides tar and cheW are separated by 144 nucleotides cheW and cheA are separated by 20 nucleotides
b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008
tap
b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715
tar
b1887: cheW - purine-binding chemotaxis protein (NCBI), at 1,970,860 to 1,971,363
cheW
b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348
cheA
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1968.203 kb on + strand, within tap at 1968.218 kb on + strand, within tap at 1968.238 kb on - strand, within tap at 1968.316 kb on + strand, within tap at 1968.329 kb on - strand, within tap at 1968.571 kb on - strand, within tap at 1968.651 kb on + strand, within tap at 1968.651 kb on + strand, within tap at 1968.660 kb on + strand, within tap at 1968.786 kb on - strand, within tap at 1968.786 kb on - strand, within tap at 1968.828 kb on - strand, within tap at 1968.852 kb on + strand at 1969.099 kb on + strand at 1969.099 kb on + strand at 1969.425 kb on - strand, within tar at 1969.705 kb on + strand, within tar at 1969.705 kb on + strand, within tar at 1969.713 kb on - strand, within tar at 1969.833 kb on + strand, within tar at 1969.925 kb on - strand, within tar at 1969.957 kb on + strand, within tar at 1970.104 kb on + strand, within tar at 1970.121 kb on - strand, within tar at 1970.185 kb on - strand, within tar at 1970.212 kb on + strand, within tar at 1970.217 kb on - strand, within tar at 1970.217 kb on - strand, within tar at 1970.267 kb on + strand, within tar at 1970.267 kb on + strand, within tar at 1970.292 kb on - strand, within tar at 1970.292 kb on - strand, within tar at 1970.319 kb on + strand, within tar at 1970.369 kb on + strand, within tar at 1970.369 kb on + strand, within tar at 1970.390 kb on - strand, within tar at 1970.488 kb on - strand, within tar at 1970.489 kb on + strand, within tar at 1970.497 kb on + strand, within tar at 1970.502 kb on + strand, within tar at 1970.602 kb on + strand at 1970.656 kb on - strand at 1970.666 kb on + strand at 1970.676 kb on + strand at 1970.765 kb on - strand at 1970.884 kb on + strand at 1970.989 kb on - strand, within cheW at 1971.037 kb on - strand, within cheW at 1971.075 kb on + strand, within cheW at 1971.116 kb on + strand, within cheW at 1971.129 kb on - strand, within cheW at 1971.140 kb on - strand, within cheW at 1971.165 kb on + strand, within cheW at 1971.165 kb on + strand, within cheW at 1971.173 kb on - strand, within cheW at 1971.247 kb on + strand, within cheW at 1971.247 kb on + strand, within cheW at 1971.250 kb on + strand, within cheW at 1971.251 kb on + strand, within cheW at 1971.251 kb on + strand, within cheW at 1971.272 kb on + strand, within cheW at 1971.275 kb on - strand, within cheW at 1971.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,968,203 + tap b1885 0.50 -0.1 1,968,218 + tap b1885 0.51 +0.8 1,968,238 - tap b1885 0.52 +0.4 1,968,316 + tap b1885 0.57 -0.8 1,968,329 - tap b1885 0.58 +0.3 1,968,571 - tap b1885 0.73 +1.5 1,968,651 + tap b1885 0.78 +0.6 1,968,651 + tap b1885 0.78 +0.1 1,968,660 + tap b1885 0.78 -1.0 1,968,786 - tap b1885 0.86 +0.0 1,968,786 - tap b1885 0.86 +0.3 1,968,828 - tap b1885 0.89 +0.1 1,968,852 + +0.2 1,969,099 + +0.6 1,969,099 + -0.2 1,969,425 - tar b1886 0.22 -0.9 1,969,705 + tar b1886 0.39 +1.2 1,969,705 + tar b1886 0.39 +0.2 1,969,713 - tar b1886 0.40 -0.9 1,969,833 + tar b1886 0.47 -1.1 1,969,925 - tar b1886 0.52 -0.1 1,969,957 + tar b1886 0.54 -1.0 1,970,104 + tar b1886 0.63 -0.8 1,970,121 - tar b1886 0.64 -0.1 1,970,185 - tar b1886 0.68 -0.7 1,970,212 + tar b1886 0.70 -0.3 1,970,217 - tar b1886 0.70 -0.1 1,970,217 - tar b1886 0.70 -0.5 1,970,267 + tar b1886 0.73 -0.1 1,970,267 + tar b1886 0.73 -1.6 1,970,292 - tar b1886 0.74 +0.5 1,970,292 - tar b1886 0.74 -1.1 1,970,319 + tar b1886 0.76 +0.9 1,970,369 + tar b1886 0.79 -0.8 1,970,369 + tar b1886 0.79 -0.3 1,970,390 - tar b1886 0.80 -0.4 1,970,488 - tar b1886 0.86 +0.6 1,970,489 + tar b1886 0.86 -0.6 1,970,497 + tar b1886 0.87 -0.1 1,970,502 + tar b1886 0.87 +0.5 1,970,602 + +0.3 1,970,656 - -0.8 1,970,666 + +0.0 1,970,676 + -0.1 1,970,765 - -0.3 1,970,884 + +0.9 1,970,989 - cheW b1887 0.26 -1.4 1,971,037 - cheW b1887 0.35 +0.4 1,971,075 + cheW b1887 0.43 +0.9 1,971,116 + cheW b1887 0.51 -0.2 1,971,129 - cheW b1887 0.53 -0.5 1,971,140 - cheW b1887 0.56 -0.8 1,971,165 + cheW b1887 0.61 +2.8 1,971,165 + cheW b1887 0.61 +1.1 1,971,173 - cheW b1887 0.62 -0.3 1,971,247 + cheW b1887 0.77 -1.5 1,971,247 + cheW b1887 0.77 -0.1 1,971,250 + cheW b1887 0.77 +0.5 1,971,251 + cheW b1887 0.78 -1.7 1,971,251 + cheW b1887 0.78 -0.5 1,971,272 + cheW b1887 0.82 +0.1 1,971,275 - cheW b1887 0.82 +0.3 1,971,539 + -0.5
Or see this region's nucleotide sequence