Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciQ and rluB are separated by 210 nucleotides rluB and btuR are separated by 39 nucleotides btuR and yciK overlap by 4 nucleotides
b1268: yciQ - predicted inner membrane protein (NCBI), at 1,322,770 to 1,324,665
yciQ
b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751
rluB
b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381
btuR
b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136
yciK
Position (kb) 1324
1325
1326 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1323.881 kb on - strand, within yciQ at 1323.883 kb on + strand, within yciQ at 1323.883 kb on + strand, within yciQ at 1323.891 kb on - strand, within yciQ at 1323.891 kb on - strand, within yciQ at 1323.891 kb on - strand, within yciQ at 1323.891 kb on - strand, within yciQ at 1323.891 kb on - strand, within yciQ at 1324.018 kb on + strand, within yciQ at 1324.018 kb on + strand, within yciQ at 1324.019 kb on - strand, within yciQ at 1324.026 kb on - strand, within yciQ at 1324.064 kb on - strand, within yciQ at 1324.131 kb on + strand, within yciQ at 1324.139 kb on - strand, within yciQ at 1324.139 kb on - strand, within yciQ at 1324.235 kb on + strand, within yciQ at 1324.236 kb on + strand, within yciQ at 1324.236 kb on + strand, within yciQ at 1324.243 kb on - strand, within yciQ at 1324.389 kb on + strand, within yciQ at 1324.389 kb on + strand, within yciQ at 1324.398 kb on - strand, within yciQ at 1324.398 kb on - strand, within yciQ at 1324.491 kb on + strand at 1324.821 kb on + strand at 1324.826 kb on + strand at 1324.829 kb on - strand at 1324.829 kb on - strand at 1325.097 kb on + strand, within rluB at 1325.110 kb on + strand, within rluB at 1325.179 kb on + strand, within rluB at 1325.179 kb on + strand, within rluB at 1325.635 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.636 kb on - strand, within rluB at 1325.814 kb on + strand at 1325.882 kb on - strand, within btuR at 1325.954 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.099 kb on - strand, within btuR at 1326.296 kb on - strand, within btuR at 1326.420 kb on + strand at 1326.420 kb on + strand at 1326.546 kb on + strand, within yciK at 1326.719 kb on + strand, within yciK at 1326.742 kb on - strand, within yciK
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,323,881 - yciQ b1268 0.59 +0.0 1,323,883 + yciQ b1268 0.59 +0.3 1,323,883 + yciQ b1268 0.59 +0.3 1,323,891 - yciQ b1268 0.59 +0.0 1,323,891 - yciQ b1268 0.59 -0.7 1,323,891 - yciQ b1268 0.59 -0.7 1,323,891 - yciQ b1268 0.59 -0.1 1,323,891 - yciQ b1268 0.59 -1.0 1,324,018 + yciQ b1268 0.66 +1.5 1,324,018 + yciQ b1268 0.66 -1.0 1,324,019 - yciQ b1268 0.66 -0.2 1,324,026 - yciQ b1268 0.66 -1.4 1,324,064 - yciQ b1268 0.68 -2.4 1,324,131 + yciQ b1268 0.72 -1.4 1,324,139 - yciQ b1268 0.72 -0.5 1,324,139 - yciQ b1268 0.72 -1.5 1,324,235 + yciQ b1268 0.77 -0.3 1,324,236 + yciQ b1268 0.77 -0.4 1,324,236 + yciQ b1268 0.77 +0.0 1,324,243 - yciQ b1268 0.78 -1.2 1,324,389 + yciQ b1268 0.85 +0.4 1,324,389 + yciQ b1268 0.85 +1.9 1,324,398 - yciQ b1268 0.86 -0.4 1,324,398 - yciQ b1268 0.86 +1.1 1,324,491 + +0.6 1,324,821 + -0.0 1,324,826 + +0.3 1,324,829 - +0.2 1,324,829 - +0.9 1,325,097 + rluB b1269 0.25 -0.9 1,325,110 + rluB b1269 0.27 -1.8 1,325,179 + rluB b1269 0.35 -1.6 1,325,179 + rluB b1269 0.35 -2.0 1,325,635 - rluB b1269 0.87 +0.6 1,325,636 - rluB b1269 0.87 -0.7 1,325,636 - rluB b1269 0.87 +1.0 1,325,814 + +0.4 1,325,882 - btuR b1270 0.15 +0.1 1,325,954 - btuR b1270 0.28 -0.8 1,326,099 - btuR b1270 0.52 +0.6 1,326,099 - btuR b1270 0.52 +1.3 1,326,296 - btuR b1270 0.85 -0.4 1,326,420 + +0.3 1,326,420 + +0.5 1,326,546 + yciK b1271 0.22 -0.3 1,326,719 + yciK b1271 0.45 +0.6 1,326,742 - yciK b1271 0.48 +0.6
Or see this region's nucleotide sequence