Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldcA and mltE are separated by 99 nucleotides mltE and ycgR are separated by 1 nucleotides ycgR and ymgE are separated by 200 nucleotides ymgE and ycgY are separated by 177 nucleotides
b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303
ldcA
b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014
mltE
b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750
ycgR
b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205
ymgE
b1196: ycgY - hypothetical protein (NCBI), at 1,244,383 to 1,244,823
ycgY
Position (kb)
1243
1244 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1242.033 kb on + strand, within ldcA at 1242.208 kb on - strand, within ldcA at 1242.237 kb on - strand at 1242.284 kb on + strand at 1242.305 kb on - strand at 1242.337 kb on + strand at 1242.355 kb on - strand at 1242.365 kb on - strand at 1242.365 kb on - strand at 1242.465 kb on + strand, within mltE at 1242.655 kb on + strand, within mltE at 1243.159 kb on + strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.521 kb on + strand, within ycgR at 1243.589 kb on + strand, within ycgR at 1243.637 kb on - strand, within ycgR at 1243.688 kb on + strand at 1243.722 kb on - strand at 1243.732 kb on + strand at 1243.798 kb on + strand at 1243.864 kb on + strand at 1243.875 kb on + strand at 1243.890 kb on + strand at 1243.893 kb on - strand at 1243.899 kb on + strand at 1243.904 kb on + strand at 1244.024 kb on - strand, within ymgE at 1244.060 kb on - strand, within ymgE at 1244.118 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.164 kb on + strand, within ymgE at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.238 kb on + strand at 1244.262 kb on + strand at 1244.267 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.336 kb on - strand at 1244.336 kb on - strand at 1244.417 kb on + strand at 1244.430 kb on - strand, within ycgY at 1244.451 kb on + strand, within ycgY at 1244.603 kb on - strand, within ycgY at 1244.645 kb on + strand, within ycgY at 1244.677 kb on - strand, within ycgY at 1244.681 kb on - strand, within ycgY at 1244.685 kb on + strand, within ycgY at 1244.685 kb on + strand, within ycgY
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,242,033 + ldcA b1192 0.70 -0.2 1,242,208 - ldcA b1192 0.90 -0.3 1,242,237 - -0.2 1,242,284 + +0.1 1,242,305 - +0.6 1,242,337 + -0.3 1,242,355 - +0.7 1,242,365 - +0.9 1,242,365 - +0.7 1,242,465 + mltE b1193 0.10 +0.3 1,242,655 + mltE b1193 0.41 -1.7 1,243,159 + ycgR b1194 0.19 -0.3 1,243,291 - ycgR b1194 0.37 -0.9 1,243,291 - ycgR b1194 0.37 -0.5 1,243,521 + ycgR b1194 0.69 -1.1 1,243,589 + ycgR b1194 0.78 +0.2 1,243,637 - ycgR b1194 0.84 -2.3 1,243,688 + +0.8 1,243,722 - -1.7 1,243,732 + -0.3 1,243,798 + +0.5 1,243,864 + -2.5 1,243,875 + -0.7 1,243,890 + +0.5 1,243,893 - -0.2 1,243,899 + -0.1 1,243,904 + -0.3 1,244,024 - ymgE b1195 0.29 +0.4 1,244,060 - ymgE b1195 0.43 -0.2 1,244,118 + ymgE b1195 0.65 -2.8 1,244,160 + ymgE b1195 0.82 +0.7 1,244,160 + ymgE b1195 0.82 -0.7 1,244,164 + ymgE b1195 0.84 -1.0 1,244,225 + +0.0 1,244,225 + -1.1 1,244,225 + +1.2 1,244,238 + +0.3 1,244,262 + -0.3 1,244,267 + -0.7 1,244,328 + -0.9 1,244,328 + -0.3 1,244,328 + +0.2 1,244,336 - -2.5 1,244,336 - -1.7 1,244,417 + +0.8 1,244,430 - ycgY b1196 0.11 +0.7 1,244,451 + ycgY b1196 0.15 +1.0 1,244,603 - ycgY b1196 0.50 -2.0 1,244,645 + ycgY b1196 0.59 -1.8 1,244,677 - ycgY b1196 0.67 +0.7 1,244,681 - ycgY b1196 0.68 -2.1 1,244,685 + ycgY b1196 0.68 -0.3 1,244,685 + ycgY b1196 0.68 +0.7
Or see this region's nucleotide sequence