Strain Fitness in Escherichia coli BW25113 around b1134

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfB and ymfB are separated by 53 nucleotidesymfB and ymfC are separated by 9 nucleotidesymfC and icd are separated by 171 nucleotides b1133: ycfB - orf, hypothetical protein (VIMSS), at 1,191,890 to 1,192,996 ycfB b1134: ymfB - bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI), at 1,193,050 to 1,193,511 ymfB b1135: ymfC - orf, hypothetical protein (VIMSS), at 1,193,521 to 1,194,174 ymfC b1136: icd - isocitrate dehydrogenase (NCBI), at 1,194,346 to 1,195,596 icd Position (kb) 1193 1194Strain fitness (log2 ratio) -1 0 1at 1193.336 kb on - strand, within ymfBat 1193.439 kb on - strand, within ymfBat 1193.469 kb on + strandat 1193.558 kb on - strandat 1193.669 kb on - strand, within ymfCat 1193.697 kb on - strand, within ymfCat 1193.774 kb on - strand, within ymfCat 1193.778 kb on + strand, within ymfCat 1193.780 kb on - strand, within ymfCat 1193.825 kb on + strand, within ymfCat 1193.886 kb on - strand, within ymfC

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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1,193,336 - ymfB b1134 0.62 -0.3
1,193,439 - ymfB b1134 0.84 +0.7
1,193,469 + -1.6
1,193,558 - -0.6
1,193,669 - ymfC b1135 0.23 -0.4
1,193,697 - ymfC b1135 0.27 +1.2
1,193,774 - ymfC b1135 0.39 +0.3
1,193,778 + ymfC b1135 0.39 -0.9
1,193,780 - ymfC b1135 0.40 +0.3
1,193,825 + ymfC b1135 0.46 -0.5
1,193,886 - ymfC b1135 0.56 +0.3

Or see this region's nucleotide sequence