Strain Fitness in Escherichia coli BW25113 around b1099

Experiment: Cisplatin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyceG and tmk overlap by 11 nucleotidestmk and holB overlap by 4 nucleotidesholB and ycfH are separated by 10 nucleotides b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357 yceG b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988 tmk b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989 holB b1100: ycfH - predicted metallodependent hydrolase (NCBI), at 1,156,000 to 1,156,797 ycfH Position (kb) 1154 1155 1156Strain fitness (log2 ratio) -2 -1 0 1 2at 1154.104 kb on - strand, within yceGat 1154.150 kb on + strand, within yceGat 1154.150 kb on + strand, within yceGat 1154.152 kb on - strand, within yceGat 1154.152 kb on - strand, within yceGat 1154.158 kb on - strand, within yceGat 1154.158 kb on - strand, within yceGat 1154.160 kb on - strand, within yceGat 1154.280 kb on - strandat 1154.323 kb on + strandat 1154.323 kb on + strandat 1155.056 kb on + strandat 1155.960 kb on + strandat 1155.960 kb on + strandat 1155.966 kb on + strandat 1156.034 kb on - strandat 1156.056 kb on + strandat 1156.056 kb on + strandat 1156.065 kb on + strandat 1156.078 kb on - strandat 1156.157 kb on + strand, within ycfHat 1156.157 kb on + strand, within ycfHat 1156.258 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.318 kb on - strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.358 kb on + strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.392 kb on - strand, within ycfHat 1156.456 kb on + strand, within ycfHat 1156.484 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.513 kb on - strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.568 kb on + strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.598 kb on - strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.602 kb on + strand, within ycfHat 1156.617 kb on + strand, within ycfHat 1156.622 kb on + strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.636 kb on - strand, within ycfHat 1156.686 kb on + strand, within ycfHat 1156.692 kb on - strand, within ycfHat 1156.696 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.698 kb on + strand, within ycfHat 1156.731 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml
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1,154,104 - yceG b1097 0.75 -0.0
1,154,150 + yceG b1097 0.80 +0.7
1,154,150 + yceG b1097 0.80 -0.8
1,154,152 - yceG b1097 0.80 -2.1
1,154,152 - yceG b1097 0.80 +1.3
1,154,158 - yceG b1097 0.80 -0.3
1,154,158 - yceG b1097 0.80 +1.1
1,154,160 - yceG b1097 0.81 -1.5
1,154,280 - +0.4
1,154,323 + -0.3
1,154,323 + -1.2
1,155,056 + -2.5
1,155,960 + -0.8
1,155,960 + -1.1
1,155,966 + -0.6
1,156,034 - -0.6
1,156,056 + -0.5
1,156,056 + -0.3
1,156,065 + -0.6
1,156,078 - +2.0
1,156,157 + ycfH b1100 0.20 +0.3
1,156,157 + ycfH b1100 0.20 +0.1
1,156,258 - ycfH b1100 0.32 -0.2
1,156,318 - ycfH b1100 0.40 +1.0
1,156,318 - ycfH b1100 0.40 +0.4
1,156,358 + ycfH b1100 0.45 -2.3
1,156,358 + ycfH b1100 0.45 +0.2
1,156,392 - ycfH b1100 0.49 -1.6
1,156,392 - ycfH b1100 0.49 +2.3
1,156,392 - ycfH b1100 0.49 -0.3
1,156,392 - ycfH b1100 0.49 +0.9
1,156,456 + ycfH b1100 0.57 -1.1
1,156,484 - ycfH b1100 0.61 -0.1
1,156,513 - ycfH b1100 0.64 +0.5
1,156,513 - ycfH b1100 0.64 +0.6
1,156,568 + ycfH b1100 0.71 -2.1
1,156,568 + ycfH b1100 0.71 +0.7
1,156,598 - ycfH b1100 0.75 +0.7
1,156,598 - ycfH b1100 0.75 +0.1
1,156,602 + ycfH b1100 0.75 +0.7
1,156,602 + ycfH b1100 0.75 +0.3
1,156,602 + ycfH b1100 0.75 +0.7
1,156,617 + ycfH b1100 0.77 +0.8
1,156,622 + ycfH b1100 0.78 +0.5
1,156,636 - ycfH b1100 0.80 -0.6
1,156,636 - ycfH b1100 0.80 +0.4
1,156,686 + ycfH b1100 0.86 +0.6
1,156,692 - ycfH b1100 0.87 +0.4
1,156,696 + ycfH b1100 0.87 +0.6
1,156,698 + ycfH b1100 0.87 +1.1
1,156,698 + ycfH b1100 0.87 -0.1
1,156,698 + ycfH b1100 0.87 +0.1
1,156,731 + +0.1

Or see this region's nucleotide sequence