Experiment: Cisplatin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rarA and ycdK are separated by 7 nucleotides ycdK and b1011 are separated by 11 nucleotides b1011 and b1012 overlap by 1 nucleotides
b1009: rarA - predicted hydrolase (NCBI), at 1,070,188 to 1,070,988
rarA
b1010: ycdK - hypothetical protein (NCBI), at 1,070,996 to 1,071,382
ycdK
b1011: b1011 - putative synthetase (VIMSS), at 1,071,394 to 1,072,086
b1011
b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234
b1012
Position (kb)
1071
1072
1073 Strain fitness (log2 ratio)
-1
0
1 at 1070.569 kb on - strand, within rarA at 1070.580 kb on - strand, within rarA at 1070.580 kb on - strand, within rarA at 1070.633 kb on - strand, within rarA at 1070.633 kb on - strand, within rarA at 1070.678 kb on - strand, within rarA at 1070.684 kb on + strand, within rarA at 1070.689 kb on + strand, within rarA at 1070.840 kb on + strand, within rarA at 1070.863 kb on - strand, within rarA at 1070.875 kb on - strand, within rarA at 1070.875 kb on - strand, within rarA at 1070.956 kb on + strand at 1070.956 kb on + strand at 1070.988 kb on + strand at 1071.230 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.319 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.382 kb on + strand at 1071.696 kb on + strand, within b1011 at 1071.712 kb on + strand, within b1011 at 1071.737 kb on - strand, within b1011 at 1072.068 kb on + strand at 1072.068 kb on + strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.188 kb on + strand at 1072.404 kb on - strand, within b1012 at 1072.404 kb on - strand, within b1012 at 1072.477 kb on - strand, within b1012 at 1072.477 kb on - strand, within b1012 at 1072.730 kb on - strand, within b1012 at 1072.755 kb on - strand, within b1012
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.05 mg/ml remove 1,070,569 - rarA b1009 0.48 +1.5 1,070,580 - rarA b1009 0.49 -0.7 1,070,580 - rarA b1009 0.49 +1.0 1,070,633 - rarA b1009 0.56 +0.6 1,070,633 - rarA b1009 0.56 +0.1 1,070,678 - rarA b1009 0.61 +0.4 1,070,684 + rarA b1009 0.62 +1.1 1,070,689 + rarA b1009 0.63 +0.6 1,070,840 + rarA b1009 0.81 -0.0 1,070,863 - rarA b1009 0.84 +0.7 1,070,875 - rarA b1009 0.86 -0.6 1,070,875 - rarA b1009 0.86 -1.0 1,070,956 + +1.0 1,070,956 + -0.0 1,070,988 + +0.7 1,071,230 - ycdK b1010 0.60 -0.5 1,071,301 - ycdK b1010 0.79 -0.3 1,071,301 - ycdK b1010 0.79 +0.1 1,071,301 - ycdK b1010 0.79 +0.3 1,071,319 - ycdK b1010 0.83 +0.7 1,071,343 - ycdK b1010 0.90 -0.3 1,071,343 - ycdK b1010 0.90 +0.7 1,071,382 + +0.6 1,071,696 + b1011 b1011 0.44 +1.2 1,071,712 + b1011 b1011 0.46 +0.6 1,071,737 - b1011 b1011 0.49 +0.2 1,072,068 + -0.9 1,072,068 + -0.3 1,072,104 - +0.2 1,072,104 - +0.4 1,072,188 + +0.7 1,072,404 - b1012 b1012 0.28 +0.4 1,072,404 - b1012 b1012 0.28 -0.5 1,072,477 - b1012 b1012 0.34 -0.4 1,072,477 - b1012 b1012 0.34 +0.4 1,072,730 - b1012 b1012 0.56 +0.1 1,072,755 - b1012 b1012 0.58 +0.0
Or see this region's nucleotide sequence